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- W2134103741 abstract "A fluorometric assay was used to study the DNA unwinding kinetics induced by Escherichia coli RecQ helicase. This assay was based on fluorescence resonance energy transfer and carried out on stopped-flow, in which DNA unwinding was monitored by fluorescence emission enhancement of fluorescein resulting from helicase-catalyzed DNA unwinding. By this method, we determined the DNA unwinding rate of RecQ at different enzyme concentrations. We also studied the dependences of DNA unwinding magnitude and rate on magnesium ion concentration. We showed that this method could be used to determine the polarity of DNA unwinding. This assay should greatly facilitate further study of the mechanism for RecQ-catalyzed DNA unwinding." @default.
- W2134103741 created "2016-06-24" @default.
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- W2134103741 date "2005-09-01" @default.
- W2134103741 modified "2023-09-24" @default.
- W2134103741 title "RecQ Helicase-catalyzed DNA Unwinding Detected by Fluorescence Resonance Energy Transfer" @default.
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- W2134103741 doi "https://doi.org/10.1111/j.1745-7270.2005.00084.x" @default.
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