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• W2134162526 startingPage "3155" @default.
• W2134162526 abstract "ABSTRACT In this study we characterized aro mutants of Salmonella enterica serovars Enteritidis and Typhimurium, which are frequently used as live oral vaccines. We found that the aroA , aroD , and aroC mutants were sensitive to blood serum, albumen, EDTA, and ovotransferrin, and this defect could be complemented by an appropriate aro gene cloned in a plasmid. Subsequent microarray analysis of gene expression in the aroD mutant in serovar Typhimurium indicated that the reason for this sensitivity might be the upregulation of murA . To confirm this, we artificially overexpressed murA from a multicopy plasmid, and this overexpression caused sensitivity of the strain to albumen and EDTA but not to serum and ovotransferrin. We concluded that attenuation of aro mutants is caused not only by their inability to synthesize aromatic metabolites but also by their defect in cell wall and outer membrane functions associated with decreased resistance to components of innate immune response." @default.
• W2134162526 created "2016-06-24" @default.
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• W2134162526 date "2008-05-01" @default.
• W2134162526 modified "2023-09-26" @default.
• W2134162526 title "<i>aro</i> Mutations in <i>Salmonella enterica</i> Cause Defects in Cell Wall and Outer Membrane Integrity" @default.
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• W2134162526 doi "https://doi.org/10.1128/jb.00053-08" @default.
• W2134162526 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2347392" @default.
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