Matches in SemOpenAlex for { <https://semopenalex.org/work/W2134184041> ?p ?o ?g. }
- W2134184041 endingPage "3432" @default.
- W2134184041 startingPage "3427" @default.
- W2134184041 abstract "ABSTRACT The availability of cloned luciferase genes from fireflies ( luc ) and from bacteria ( luxAB ) has led to the widespread use of bioluminescence as a reporter to measure cell viability and gene expression. The most commonly occurring bioluminescence system in nature is the deep-sea imidazolopyrazine bioluminescence system. Coelenterazine is an imidazolopyrazine derivative which, when oxidized by an appropriate luciferase enzyme, produces carbon dioxide, coelenteramide, and light. The luciferase from the marine copepod Gaussia princeps ( Gluc ) has recently been cloned. We expressed the Gluc gene in Mycobacterium smegmatis using a shuttle vector and compared its performance with that of an existing luxAB reporter. In contrast to luxAB , the Gluc luciferase retained its luminescence output in the stationary phase of growth and exhibited enhanced stability during exposure to low pH, hydrogen peroxide, and high temperature. The work presented here demonstrated the utility of the copepod luciferase bioluminescent reporter as an alternative to bacterial luciferase, particularly for monitoring responses to environmental stress stimuli." @default.
- W2134184041 created "2016-06-24" @default.
- W2134184041 creator A5001202729 @default.
- W2134184041 creator A5037112838 @default.
- W2134184041 creator A5048343643 @default.
- W2134184041 creator A5050328406 @default.
- W2134184041 creator A5081343594 @default.
- W2134184041 date "2005-07-01" @default.
- W2134184041 modified "2023-09-27" @default.
- W2134184041 title "Alternative Luciferase for Monitoring Bacterial Cells under Adverse Conditions" @default.
- W2134184041 cites W100921073 @default.
- W2134184041 cites W1485211345 @default.
- W2134184041 cites W1497666841 @default.
- W2134184041 cites W1500998580 @default.
- W2134184041 cites W1551721102 @default.
- W2134184041 cites W1591311438 @default.
- W2134184041 cites W1591968233 @default.
- W2134184041 cites W1649997932 @default.
- W2134184041 cites W1960714279 @default.
- W2134184041 cites W1962278961 @default.
- W2134184041 cites W1964922019 @default.
- W2134184041 cites W1965715660 @default.
- W2134184041 cites W1967457339 @default.
- W2134184041 cites W1985316610 @default.
- W2134184041 cites W1998026338 @default.
- W2134184041 cites W1998810321 @default.
- W2134184041 cites W2003550623 @default.
- W2134184041 cites W2011534105 @default.
- W2134184041 cites W2021300094 @default.
- W2134184041 cites W2028064214 @default.
- W2134184041 cites W2031649195 @default.
- W2134184041 cites W2035893701 @default.
- W2134184041 cites W2037178607 @default.
- W2134184041 cites W2048468622 @default.
- W2134184041 cites W2048512273 @default.
- W2134184041 cites W2060811233 @default.
- W2134184041 cites W2061579546 @default.
- W2134184041 cites W2062758687 @default.
- W2134184041 cites W2069844653 @default.
- W2134184041 cites W2075069619 @default.
- W2134184041 cites W2081386092 @default.
- W2134184041 cites W2082573007 @default.
- W2134184041 cites W2083553607 @default.
- W2134184041 cites W2091206106 @default.
- W2134184041 cites W2091489806 @default.
- W2134184041 cites W2093704778 @default.
- W2134184041 cites W2100441327 @default.
- W2134184041 cites W2102208377 @default.
- W2134184041 cites W2105063350 @default.
- W2134184041 cites W2106685495 @default.
- W2134184041 cites W2109794591 @default.
- W2134184041 cites W2112226533 @default.
- W2134184041 cites W2112256627 @default.
- W2134184041 cites W2113360966 @default.
- W2134184041 cites W2114677785 @default.
- W2134184041 cites W2124477052 @default.
- W2134184041 cites W2126913947 @default.
- W2134184041 cites W2127824155 @default.
- W2134184041 cites W2129006543 @default.
- W2134184041 cites W2130832985 @default.
- W2134184041 cites W2134400105 @default.
- W2134184041 cites W2135068530 @default.
- W2134184041 cites W2139387392 @default.
- W2134184041 cites W2141619262 @default.
- W2134184041 cites W2146548811 @default.
- W2134184041 cites W2150300378 @default.
- W2134184041 cites W2156129359 @default.
- W2134184041 cites W2156867700 @default.
- W2134184041 cites W2157737997 @default.
- W2134184041 cites W2161877479 @default.
- W2134184041 cites W2166184949 @default.
- W2134184041 cites W2168895407 @default.
- W2134184041 cites W2220844760 @default.
- W2134184041 cites W2436160748 @default.
- W2134184041 cites W2443090232 @default.
- W2134184041 cites W2486296767 @default.
- W2134184041 doi "https://doi.org/10.1128/aem.71.7.3427-3432.2005" @default.
- W2134184041 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1169068" @default.
- W2134184041 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16000745" @default.
- W2134184041 hasPublicationYear "2005" @default.
- W2134184041 type Work @default.
- W2134184041 sameAs 2134184041 @default.
- W2134184041 citedByCount "54" @default.
- W2134184041 countsByYear W21341840412012 @default.
- W2134184041 countsByYear W21341840412013 @default.
- W2134184041 countsByYear W21341840412014 @default.
- W2134184041 countsByYear W21341840412015 @default.
- W2134184041 countsByYear W21341840412016 @default.
- W2134184041 countsByYear W21341840412017 @default.
- W2134184041 countsByYear W21341840412018 @default.
- W2134184041 countsByYear W21341840412019 @default.
- W2134184041 countsByYear W21341840412020 @default.
- W2134184041 countsByYear W21341840412021 @default.
- W2134184041 countsByYear W21341840412022 @default.
- W2134184041 crossrefType "journal-article" @default.
- W2134184041 hasAuthorship W2134184041A5001202729 @default.
- W2134184041 hasAuthorship W2134184041A5037112838 @default.
- W2134184041 hasAuthorship W2134184041A5048343643 @default.