Matches in SemOpenAlex for { <https://semopenalex.org/work/W2134203604> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2134203604 endingPage "153" @default.
- W2134203604 startingPage "144" @default.
- W2134203604 abstract "An improved differential fluorescence analysis technique which employs scanning laser cytometry for the detection of two DNA binding fluorochromes was used to quantitate both total DNA and cellular incorporation of the base analogue, bromodeoxyuridine (BrdUrd), into UV- or methyl methane sulphonate (MMS)-treated DNA. In this procedure DNA containing BrdUrd was partially denatured and immunolabeled by binding anti-BrdUrd monoclonal IgG to incorporated BrdUrd. Anti-BrdUrd was detected with a secondary fluorochromelabeled polyclonal anti-IgG. Cells were counterstained with propidium iodide to allow the determination of total DNA. Fluorescence was determined using the Meridian ACAS 570 Scanning Laser Cytometer. The computer-analyzed images were generated from monodisperse populations of UV- or MMS-treated cells, allowing the evaluation of DNA synthesis associated with excision repair and of total DNA content in single cells. These data were compared with 3H-thymidine incorporation occurring as a function of excision repair. The analysis of DNA synthesis with this technique is consistent, relatively simple, and rapid with excellent sensitivity and provides a viable method for determining cycling vs. non-cycling cells, total cellular DNA, and excision repair-associated DNA synthesis of individual cells within heterogeneous cell populations. © 1993 Wiley-Liss, Inc." @default.
- W2134203604 created "2016-06-24" @default.
- W2134203604 creator A5005774022 @default.
- W2134203604 creator A5018422490 @default.
- W2134203604 creator A5055012486 @default.
- W2134203604 date "1993-01-01" @default.
- W2134203604 modified "2023-09-24" @default.
- W2134203604 title "Immunofluorescent evaluation of DNA repair synthesis using interactive laser cytometry" @default.
- W2134203604 cites W1508389014 @default.
- W2134203604 cites W1554627281 @default.
- W2134203604 cites W1964315344 @default.
- W2134203604 cites W1965917766 @default.
- W2134203604 cites W1971179628 @default.
- W2134203604 cites W1975173902 @default.
- W2134203604 cites W1984635932 @default.
- W2134203604 cites W1985260716 @default.
- W2134203604 cites W1985684591 @default.
- W2134203604 cites W1999376589 @default.
- W2134203604 cites W2002236717 @default.
- W2134203604 cites W2022658882 @default.
- W2134203604 cites W2028786681 @default.
- W2134203604 cites W2031022560 @default.
- W2134203604 cites W2031468048 @default.
- W2134203604 cites W2034032778 @default.
- W2134203604 cites W2034820400 @default.
- W2134203604 cites W2059195500 @default.
- W2134203604 cites W2061826894 @default.
- W2134203604 cites W2062742761 @default.
- W2134203604 cites W2066140090 @default.
- W2134203604 cites W2069958185 @default.
- W2134203604 cites W2077337170 @default.
- W2134203604 cites W2082127218 @default.
- W2134203604 cites W2083126860 @default.
- W2134203604 cites W2085879212 @default.
- W2134203604 cites W2086790640 @default.
- W2134203604 cites W2090566390 @default.
- W2134203604 cites W2090761087 @default.
- W2134203604 cites W2092396004 @default.
- W2134203604 cites W2130378981 @default.
- W2134203604 cites W2158583522 @default.
- W2134203604 cites W2163522629 @default.
- W2134203604 cites W2174274173 @default.
- W2134203604 cites W2313773626 @default.
- W2134203604 cites W4233018052 @default.
- W2134203604 doi "https://doi.org/10.1002/cyto.990140206" @default.
- W2134203604 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8440148" @default.
- W2134203604 hasPublicationYear "1993" @default.
- W2134203604 type Work @default.
- W2134203604 sameAs 2134203604 @default.
- W2134203604 citedByCount "6" @default.
- W2134203604 countsByYear W21342036042012 @default.
- W2134203604 crossrefType "journal-article" @default.
- W2134203604 hasAuthorship W2134203604A5005774022 @default.
- W2134203604 hasAuthorship W2134203604A5018422490 @default.
- W2134203604 hasAuthorship W2134203604A5055012486 @default.
- W2134203604 hasBestOaLocation W21342036041 @default.
- W2134203604 hasConcept C134935766 @default.
- W2134203604 hasConcept C153911025 @default.
- W2134203604 hasConcept C190283241 @default.
- W2134203604 hasConcept C2775934118 @default.
- W2134203604 hasConcept C2778260397 @default.
- W2134203604 hasConcept C31573885 @default.
- W2134203604 hasConcept C552990157 @default.
- W2134203604 hasConcept C553184892 @default.
- W2134203604 hasConcept C55493867 @default.
- W2134203604 hasConcept C62112901 @default.
- W2134203604 hasConcept C86803240 @default.
- W2134203604 hasConceptScore W2134203604C134935766 @default.
- W2134203604 hasConceptScore W2134203604C153911025 @default.
- W2134203604 hasConceptScore W2134203604C190283241 @default.
- W2134203604 hasConceptScore W2134203604C2775934118 @default.
- W2134203604 hasConceptScore W2134203604C2778260397 @default.
- W2134203604 hasConceptScore W2134203604C31573885 @default.
- W2134203604 hasConceptScore W2134203604C552990157 @default.
- W2134203604 hasConceptScore W2134203604C553184892 @default.
- W2134203604 hasConceptScore W2134203604C55493867 @default.
- W2134203604 hasConceptScore W2134203604C62112901 @default.
- W2134203604 hasConceptScore W2134203604C86803240 @default.
- W2134203604 hasIssue "2" @default.
- W2134203604 hasLocation W21342036041 @default.
- W2134203604 hasLocation W21342036042 @default.
- W2134203604 hasOpenAccess W2134203604 @default.
- W2134203604 hasPrimaryLocation W21342036041 @default.
- W2134203604 hasRelatedWork W1967215172 @default.
- W2134203604 hasRelatedWork W1980648174 @default.
- W2134203604 hasRelatedWork W1992079809 @default.
- W2134203604 hasRelatedWork W1992123084 @default.
- W2134203604 hasRelatedWork W2019637051 @default.
- W2134203604 hasRelatedWork W2021896669 @default.
- W2134203604 hasRelatedWork W2046292631 @default.
- W2134203604 hasRelatedWork W2046792340 @default.
- W2134203604 hasRelatedWork W2049187149 @default.
- W2134203604 hasRelatedWork W2099182300 @default.
- W2134203604 hasVolume "14" @default.
- W2134203604 isParatext "false" @default.
- W2134203604 isRetracted "false" @default.
- W2134203604 magId "2134203604" @default.
- W2134203604 workType "article" @default.