Matches in SemOpenAlex for { <https://semopenalex.org/work/W2134394930> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2134394930 endingPage "2265" @default.
- W2134394930 startingPage "2259" @default.
- W2134394930 abstract "Oligonucleotide microchips are manufactured by immobilizing presynthesized oligonucleotides within 0.1 × 0.1 × 0.02 mm or 1 × 1 × 0.02 mm polyacrylamide gel pads arranged on the surface of a microscope slide. The gel pads are separated from each other by hydrophobic glass spacers and serve as a kind of ‘microtest tube’ of 200 pl or 20 nl volume, respectively. Fractionation of single-stranded DNAs is carried out by their hybridization with chip pads containing immobilized 10mers. DNA extracted separately from each pad is transferred onto a sequencing chip and analyzed thereon. The chip, containing a set of 10mers, was enzymatically phosphorylated, then hybridized with DNA and ligated in a site-directed manner with a contiguously stacked 5mer. Several cycles of successive hybridization-ligation of the chip-bound 10mers with different contiguously stacked 5mers and hybridized with DNA were carried out to sequence DNA containing tetranucleotide repeats. Combined use of these techniques show significant promise for sequence comparison of homologous regions in different genomes and for sequence analysis of comparatively long DNA fragments or DNA containing internal repeats." @default.
- W2134394930 created "2016-06-24" @default.
- W2134394930 creator A5018496921 @default.
- W2134394930 creator A5024063403 @default.
- W2134394930 creator A5060380623 @default.
- W2134394930 creator A5030626377 @default.
- W2134394930 date "1997-06-01" @default.
- W2134394930 modified "2023-09-25" @default.
- W2134394930 title "Fractionation, phosphorylation and ligation on oligonucleotide microchips to enhance sequencing by hybridization" @default.
- W2134394930 cites W1968828650 @default.
- W2134394930 cites W1975188059 @default.
- W2134394930 cites W1981691571 @default.
- W2134394930 cites W1983183354 @default.
- W2134394930 cites W1985080632 @default.
- W2134394930 cites W1986088012 @default.
- W2134394930 cites W1987690788 @default.
- W2134394930 cites W1994098923 @default.
- W2134394930 cites W2021606973 @default.
- W2134394930 cites W2037509330 @default.
- W2134394930 cites W2043163815 @default.
- W2134394930 cites W2056292544 @default.
- W2134394930 cites W2056447265 @default.
- W2134394930 cites W2059667789 @default.
- W2134394930 cites W2075527656 @default.
- W2134394930 cites W2077095651 @default.
- W2134394930 cites W2087006382 @default.
- W2134394930 cites W2102353334 @default.
- W2134394930 cites W2107295946 @default.
- W2134394930 cites W2146986076 @default.
- W2134394930 cites W2159511216 @default.
- W2134394930 cites W4253485643 @default.
- W2134394930 doi "https://doi.org/10.1093/nar/25.12.2259" @default.
- W2134394930 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/146758" @default.
- W2134394930 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9171075" @default.
- W2134394930 hasPublicationYear "1997" @default.
- W2134394930 type Work @default.
- W2134394930 sameAs 2134394930 @default.
- W2134394930 citedByCount "54" @default.
- W2134394930 countsByYear W21343949302016 @default.
- W2134394930 countsByYear W21343949302018 @default.
- W2134394930 crossrefType "journal-article" @default.
- W2134394930 hasAuthorship W2134394930A5018496921 @default.
- W2134394930 hasAuthorship W2134394930A5024063403 @default.
- W2134394930 hasAuthorship W2134394930A5030626377 @default.
- W2134394930 hasAuthorship W2134394930A5060380623 @default.
- W2134394930 hasBestOaLocation W21343949301 @default.
- W2134394930 hasConcept C116084860 @default.
- W2134394930 hasConcept C129312508 @default.
- W2134394930 hasConcept C153911025 @default.
- W2134394930 hasConcept C179437574 @default.
- W2134394930 hasConcept C3017666073 @default.
- W2134394930 hasConcept C51064596 @default.
- W2134394930 hasConcept C51679486 @default.
- W2134394930 hasConcept C54871578 @default.
- W2134394930 hasConcept C552990157 @default.
- W2134394930 hasConcept C55493867 @default.
- W2134394930 hasConcept C70712619 @default.
- W2134394930 hasConcept C86803240 @default.
- W2134394930 hasConceptScore W2134394930C116084860 @default.
- W2134394930 hasConceptScore W2134394930C129312508 @default.
- W2134394930 hasConceptScore W2134394930C153911025 @default.
- W2134394930 hasConceptScore W2134394930C179437574 @default.
- W2134394930 hasConceptScore W2134394930C3017666073 @default.
- W2134394930 hasConceptScore W2134394930C51064596 @default.
- W2134394930 hasConceptScore W2134394930C51679486 @default.
- W2134394930 hasConceptScore W2134394930C54871578 @default.
- W2134394930 hasConceptScore W2134394930C552990157 @default.
- W2134394930 hasConceptScore W2134394930C55493867 @default.
- W2134394930 hasConceptScore W2134394930C70712619 @default.
- W2134394930 hasConceptScore W2134394930C86803240 @default.
- W2134394930 hasIssue "12" @default.
- W2134394930 hasLocation W21343949301 @default.
- W2134394930 hasLocation W21343949302 @default.
- W2134394930 hasLocation W21343949303 @default.
- W2134394930 hasLocation W21343949304 @default.
- W2134394930 hasOpenAccess W2134394930 @default.
- W2134394930 hasPrimaryLocation W21343949301 @default.
- W2134394930 hasRelatedWork W1562501921 @default.
- W2134394930 hasRelatedWork W1969973549 @default.
- W2134394930 hasRelatedWork W1979197188 @default.
- W2134394930 hasRelatedWork W2056583959 @default.
- W2134394930 hasRelatedWork W2059667789 @default.
- W2134394930 hasRelatedWork W2097253516 @default.
- W2134394930 hasRelatedWork W2130995160 @default.
- W2134394930 hasRelatedWork W2151200650 @default.
- W2134394930 hasRelatedWork W2405754692 @default.
- W2134394930 hasRelatedWork W4290195070 @default.
- W2134394930 hasVolume "25" @default.
- W2134394930 isParatext "false" @default.
- W2134394930 isRetracted "false" @default.
- W2134394930 magId "2134394930" @default.
- W2134394930 workType "article" @default.