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- W2134513791 abstract "Mitochondrial superoxide flash is a newly discovered physiological phenomenon reflecting elemental and bursting superoxide production in mitochondria of diverse cells in culture and in vivo (Wang et al, Cell, 132, 279).The discovery of superoxide flash opens a unique window to glean into the mitochondrial ROS signaling and its coupling with energy metabolism,cell fate regulation, and oxidative stress-related diseases. Because of the low frequency and long duration (∼20s) of superoxide flash, time-lapse confocal imaging (xyt) was employed for data acquisition, resulting in huge data sets. For objective, reproducible and efficient flash identification and measurement, here we develop, validate and implement an automated detection algorithm as well as a software, Flash Sniper, which fulfills three basic functions -- interactive data priming, flash identification, and flash parameter measurement. The data priming module consists of correction for photobleach and photoconversion using nonlinear fitting, cell motion compensation based on hierarchical block matching, and spatiotemporal filtering. Two xy maps are generated to represent the maxima of fluorescent intensity change and the corresponding time, respectively. Flash events are identified via thresholding and segmentation based on these maps. Validation of the algorithm is performed on SNR-controlled synthetic data sets produced by embedding typical flash events in real images. With the aid of Flash Sniper, we examined ∼1600 flashes in cardiac myocytes and revealed distinct substructures in their rising kinetics. In sum, the analytical tool developed in this study will facilitate the studies of the mechanisms of superoxide flash and its application in health and diseases." @default.
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- W2134513791 date "2009-02-01" @default.
- W2134513791 modified "2023-10-16" @default.
- W2134513791 title "Flash Sniper: Automated Detection and Analysis of Mitochondrial Superoxide Flash" @default.
- W2134513791 doi "https://doi.org/10.1016/j.bpj.2008.12.2743" @default.
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