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• W2134568507 abstract "Development of epithelial cell polarity is a highly dynamic process, and often established by the sequential recruitment of conserved protein complexes, such as the Par or the Crumbs (Crb) complex. However, detailed insights into the refinement of polarity and the formation of the complexes are still lacking. Here, we established fluorescently tagged Lin7c, a core member of the Crb complex, as an ideal tool to follow development of polarity in zebrafish epithelia. We find that in gastrula stages, RFP-Lin7c is found in the cytosol of the enveloping layer, while Pard3-GFP is already polarized at this stage. During development of the retinal epithelium, RFP-Lin7c localization is refined from being cytosolic at 14 hours post fertilization (hpf) to almost entirely apical in cells of the eye cup at 28 hpf. This apical Lin7c localization depends on the Crb complex members Oko meduzy and Nagie oko. Thus, fluorescently tagged Lin7c can be used in a broad range of epithelia to follow polarity maturation in vivo and specifically to elucidate the sequence of events determining Crb complex-mediated polarity." @default.
• W2134568507 created "2016-06-24" @default.
• W2134568507 creator A5002595262 @default.
• W2134568507 creator A5055110576 @default.
• W2134568507 date "2013-07-01" @default.
• W2134568507 modified "2023-09-24" @default.
• W2134568507 title "Fluorescently tagged Lin7c is a dynamic marker for polarity maturation in the zebrafish retinal epithelium" @default.
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• W2134568507 doi "https://doi.org/10.1242/bio.20135371" @default.
• W2134568507 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3773332" @default.
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