Matches in SemOpenAlex for { <https://semopenalex.org/work/W2134612794> ?p ?o ?g. }
- W2134612794 endingPage "6089" @default.
- W2134612794 startingPage "6082" @default.
- W2134612794 abstract "mAb NL7 was raised against purified flavocytochrome b(558), important in host defense and inflammation. NL7 recognized the gp91(phox) flavocytochrome b(558) subunit by immunoblot and bound to permeabilized neutrophils and neutrophil membranes. Epitope mapping by phage display analysis indicated that NL7 binds the (498)EKDVITGLK(506) region of gp91(phox). In a cell-free assay, NL7 inhibited in vitro activation of the NADPH oxidase in a concentration-dependent manner, and had marginal effects on the oxidase substrate Michaelis constant (K(m)). mAb NL7 did not inhibit translocation of p47(phox), p67(phox), or Rac to the plasma membrane, and bound its epitope on gp91(phox) independently of cytosolic factor translocation. However, after assembly of the NADPH oxidase complex, mAb NL7 bound the epitope but did not inhibit the generation of superoxide. Three-dimensional modeling of the C-terminal domain of gp91(phox) on a corn nitrate reductase template suggests close proximity of the NL7 epitope to the proposed NADPH binding site, but significant separation from the proposed p47(phox) binding sites. We conclude that the (498)EKDVITGLK(506) segment resides on the cytosolic surface of gp91(phox) and represents a region important for oxidase function, but not substrate or cytosolic component binding." @default.
- W2134612794 created "2016-06-24" @default.
- W2134612794 creator A5007589576 @default.
- W2134612794 creator A5031182133 @default.
- W2134612794 creator A5031455820 @default.
- W2134612794 creator A5039396452 @default.
- W2134612794 creator A5050922635 @default.
- W2134612794 creator A5052602246 @default.
- W2134612794 creator A5055061299 @default.
- W2134612794 creator A5072881759 @default.
- W2134612794 creator A5074708797 @default.
- W2134612794 creator A5079948516 @default.
- W2134612794 date "2003-06-15" @default.
- W2134612794 modified "2023-09-30" @default.
- W2134612794 title "Functional Epitope on Human Neutrophil Flavocytochrome <i>b</i>558" @default.
- W2134612794 cites W1481009887 @default.
- W2134612794 cites W1481238256 @default.
- W2134612794 cites W1485049172 @default.
- W2134612794 cites W1499539705 @default.
- W2134612794 cites W1537144225 @default.
- W2134612794 cites W1544869976 @default.
- W2134612794 cites W1566004432 @default.
- W2134612794 cites W1566061018 @default.
- W2134612794 cites W1566587067 @default.
- W2134612794 cites W1589174107 @default.
- W2134612794 cites W1591942886 @default.
- W2134612794 cites W1810573536 @default.
- W2134612794 cites W185538403 @default.
- W2134612794 cites W1959078926 @default.
- W2134612794 cites W1970742573 @default.
- W2134612794 cites W1978727971 @default.
- W2134612794 cites W1981099635 @default.
- W2134612794 cites W1981574104 @default.
- W2134612794 cites W1982581618 @default.
- W2134612794 cites W1983635965 @default.
- W2134612794 cites W1983762825 @default.
- W2134612794 cites W1989764592 @default.
- W2134612794 cites W1995804959 @default.
- W2134612794 cites W1997442725 @default.
- W2134612794 cites W2004201752 @default.
- W2134612794 cites W2008981570 @default.
- W2134612794 cites W2019640011 @default.
- W2134612794 cites W2026358430 @default.
- W2134612794 cites W2043543278 @default.
- W2134612794 cites W2050378706 @default.
- W2134612794 cites W2065576762 @default.
- W2134612794 cites W2068371198 @default.
- W2134612794 cites W2069179157 @default.
- W2134612794 cites W2078744612 @default.
- W2134612794 cites W2084466582 @default.
- W2134612794 cites W2092234562 @default.
- W2134612794 cites W2108499829 @default.
- W2134612794 cites W2119518322 @default.
- W2134612794 cites W2135358834 @default.
- W2134612794 cites W2143908574 @default.
- W2134612794 cites W2149851012 @default.
- W2134612794 cites W2163785575 @default.
- W2134612794 cites W2168308945 @default.
- W2134612794 cites W2276471975 @default.
- W2134612794 cites W2399591470 @default.
- W2134612794 cites W4251515906 @default.
- W2134612794 doi "https://doi.org/10.4049/jimmunol.170.12.6082" @default.
- W2134612794 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12794137" @default.
- W2134612794 hasPublicationYear "2003" @default.
- W2134612794 type Work @default.
- W2134612794 sameAs 2134612794 @default.
- W2134612794 citedByCount "43" @default.
- W2134612794 countsByYear W21346127942012 @default.
- W2134612794 countsByYear W21346127942014 @default.
- W2134612794 countsByYear W21346127942015 @default.
- W2134612794 countsByYear W21346127942018 @default.
- W2134612794 countsByYear W21346127942019 @default.
- W2134612794 countsByYear W21346127942021 @default.
- W2134612794 countsByYear W21346127942022 @default.
- W2134612794 countsByYear W21346127942023 @default.
- W2134612794 crossrefType "journal-article" @default.
- W2134612794 hasAuthorship W2134612794A5007589576 @default.
- W2134612794 hasAuthorship W2134612794A5031182133 @default.
- W2134612794 hasAuthorship W2134612794A5031455820 @default.
- W2134612794 hasAuthorship W2134612794A5039396452 @default.
- W2134612794 hasAuthorship W2134612794A5050922635 @default.
- W2134612794 hasAuthorship W2134612794A5052602246 @default.
- W2134612794 hasAuthorship W2134612794A5055061299 @default.
- W2134612794 hasAuthorship W2134612794A5072881759 @default.
- W2134612794 hasAuthorship W2134612794A5074708797 @default.
- W2134612794 hasAuthorship W2134612794A5079948516 @default.
- W2134612794 hasBestOaLocation W21346127941 @default.
- W2134612794 hasConcept C104292427 @default.
- W2134612794 hasConcept C104317684 @default.
- W2134612794 hasConcept C107824862 @default.
- W2134612794 hasConcept C147483822 @default.
- W2134612794 hasConcept C153911025 @default.
- W2134612794 hasConcept C159654299 @default.
- W2134612794 hasConcept C181199279 @default.
- W2134612794 hasConcept C185592680 @default.
- W2134612794 hasConcept C195616568 @default.
- W2134612794 hasConcept C203014093 @default.
- W2134612794 hasConcept C2779719074 @default.