Matches in SemOpenAlex for { <https://semopenalex.org/work/W2134700543> ?p ?o ?g. }
- W2134700543 endingPage "4797" @default.
- W2134700543 startingPage "4789" @default.
- W2134700543 abstract "The direct involvement of melatonin in modulation of ovarian steroidogenesis, the high levels of melatonin found in human follicular fluid, and the presence of melatonin binding sites in the ovary led us to hypothesize that melatonin acts as a modulator of ovarian function. In contrast to the hypothalamus and pituitary, the mechanism of melatonin action at the level of the ovary is still poorly understood. In the present study, we investigated the gene expression of the two different forms of melatonin receptors in human granulosa-luteal cells, using RT-PCR. PCR products corresponding to the expected sizes of the melatonin receptor subtypes, mt1-R and MT2-R, were obtained from granulosa-luteal cells, and the authenticity of the PCR products was confirmed by Southern blot hybridization with cDNA probes. Subsequent cloning and sequence analysis revealed that the ovarian mt1-R and MT2-R cDNAs are identical to their brain counterparts. Because gonadotropins and GnRH acting through specific receptors in the human ovary regulate cellular functions, we investigated the role of melatonin in the regulation of FSH receptor, LH receptor, GnRH, and GnRH receptor levels. Treatment with melatonin (10 pm–100 nm) significantly increased LH receptor mRNA levels without altering the expression of the FSH receptor gene. Both GnRH and GnRH receptor mRNA levels were significantly decreased, to 61% and 45% of control levels, respectively, after melatonin treatment. Melatonin treatment alone had no effect on basal progesterone production but enhanced the effects of human CG-stimulated progesterone production. Because MAPKs are activated in response to a diverse array of extracellular stimuli leading to the regulation of cell growth, division, and differentiation, and because melatonin has been shown to modulate cellular proliferation and differentiation, in this study, we demonstrated that melatonin activated MAPK in a dose- and time-dependent manner. In summary, our studies demonstrate, for the first time, that melatonin can regulate progesterone production, LH receptor, GnRH, and GnRH receptor gene expression through melatonin receptors in human granulosa-luteal cells, which may be mediated via the MAPK pathway and activation of Elk-1. Our results support the notion that melatonin plays a direct role in regulating ovarian function." @default.
- W2134700543 created "2016-06-24" @default.
- W2134700543 creator A5024860849 @default.
- W2134700543 creator A5047119049 @default.
- W2134700543 creator A5049010829 @default.
- W2134700543 creator A5059854070 @default.
- W2134700543 creator A5083611772 @default.
- W2134700543 creator A5083931965 @default.
- W2134700543 date "2001-10-01" @default.
- W2134700543 modified "2023-10-18" @default.
- W2134700543 title "Direct Action of Melatonin in Human Granulosa-Luteal Cells" @default.
- W2134700543 cites W1558696226 @default.
- W2134700543 cites W1572746674 @default.
- W2134700543 cites W1840795163 @default.
- W2134700543 cites W1964586431 @default.
- W2134700543 cites W1966384215 @default.
- W2134700543 cites W1981794200 @default.
- W2134700543 cites W1982186905 @default.
- W2134700543 cites W1984300828 @default.
- W2134700543 cites W1987313886 @default.
- W2134700543 cites W1993266683 @default.
- W2134700543 cites W1996068610 @default.
- W2134700543 cites W2003910569 @default.
- W2134700543 cites W2004208175 @default.
- W2134700543 cites W2006462635 @default.
- W2134700543 cites W2010321144 @default.
- W2134700543 cites W2010523538 @default.
- W2134700543 cites W2010692240 @default.
- W2134700543 cites W2012028784 @default.
- W2134700543 cites W2012428547 @default.
- W2134700543 cites W2017440399 @default.
- W2134700543 cites W2024736233 @default.
- W2134700543 cites W2027418315 @default.
- W2134700543 cites W2031148940 @default.
- W2134700543 cites W2032069140 @default.
- W2134700543 cites W2034348289 @default.
- W2134700543 cites W2034963335 @default.
- W2134700543 cites W2036206595 @default.
- W2134700543 cites W2037954942 @default.
- W2134700543 cites W2045103809 @default.
- W2134700543 cites W2048542290 @default.
- W2134700543 cites W2072294194 @default.
- W2134700543 cites W2072317433 @default.
- W2134700543 cites W2074425921 @default.
- W2134700543 cites W2074694674 @default.
- W2134700543 cites W2076340639 @default.
- W2134700543 cites W2076550840 @default.
- W2134700543 cites W2090044308 @default.
- W2134700543 cites W2091385411 @default.
- W2134700543 cites W2092156741 @default.
- W2134700543 cites W2094654195 @default.
- W2134700543 cites W2100837269 @default.
- W2134700543 cites W2101108802 @default.
- W2134700543 cites W2101597908 @default.
- W2134700543 cites W2102514279 @default.
- W2134700543 cites W2117556333 @default.
- W2134700543 cites W2128642080 @default.
- W2134700543 cites W2131391777 @default.
- W2134700543 cites W2134812217 @default.
- W2134700543 cites W2136052981 @default.
- W2134700543 cites W2136350449 @default.
- W2134700543 cites W2171853178 @default.
- W2134700543 cites W2411129294 @default.
- W2134700543 cites W2415500772 @default.
- W2134700543 cites W2471230567 @default.
- W2134700543 cites W2920878173 @default.
- W2134700543 cites W70834850 @default.
- W2134700543 cites W912507531 @default.
- W2134700543 cites W2160124934 @default.
- W2134700543 doi "https://doi.org/10.1210/jcem.86.10.7912" @default.
- W2134700543 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11600542" @default.
- W2134700543 hasPublicationYear "2001" @default.
- W2134700543 type Work @default.
- W2134700543 sameAs 2134700543 @default.
- W2134700543 citedByCount "171" @default.
- W2134700543 countsByYear W21347005432012 @default.
- W2134700543 countsByYear W21347005432013 @default.
- W2134700543 countsByYear W21347005432014 @default.
- W2134700543 countsByYear W21347005432015 @default.
- W2134700543 countsByYear W21347005432016 @default.
- W2134700543 countsByYear W21347005432017 @default.
- W2134700543 countsByYear W21347005432018 @default.
- W2134700543 countsByYear W21347005432019 @default.
- W2134700543 countsByYear W21347005432020 @default.
- W2134700543 countsByYear W21347005432021 @default.
- W2134700543 countsByYear W21347005432022 @default.
- W2134700543 countsByYear W21347005432023 @default.
- W2134700543 crossrefType "journal-article" @default.
- W2134700543 hasAuthorship W2134700543A5024860849 @default.
- W2134700543 hasAuthorship W2134700543A5047119049 @default.
- W2134700543 hasAuthorship W2134700543A5049010829 @default.
- W2134700543 hasAuthorship W2134700543A5059854070 @default.
- W2134700543 hasAuthorship W2134700543A5083611772 @default.
- W2134700543 hasAuthorship W2134700543A5083931965 @default.
- W2134700543 hasBestOaLocation W21347005431 @default.
- W2134700543 hasConcept C126322002 @default.
- W2134700543 hasConcept C134018914 @default.
- W2134700543 hasConcept C143228043 @default.