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• W2134711862 endingPage "725" @default.
• W2134711862 startingPage "715" @default.
• W2134711862 abstract "Substrate channeling is a process of transferring the product of one enzyme to an adjacent cascade enzyme or cell without complete mixing with the bulk phase. Such phenomena can occur in vivo, in vitro, or ex vivo. Enzyme–enzyme or enzyme–cell complexes may be static or transient. In addition to enhanced reaction rates through substrate channeling in complexes, numerous potential benefits of such complexes are protection of unstable substrates, circumvention of unfavorable equilibrium and kinetics imposed, forestallment of substrate competition among different pathways, regulation of metabolic fluxes, mitigation of toxic metabolite inhibition, and so on. Here we review numerous examples of natural and synthetic complexes featuring substrate channeling. Constructing synthetic in vivo, in vitro or ex vivo complexes for substrate channeling would have great biotechnological potentials in metabolic engineering, multi-enzyme-mediated biocatalysis, and cell-free synthetic pathway biotransformation (SyPaB)." @default.
• W2134711862 created "2016-06-24" @default.
• W2134711862 creator A5026324655 @default.
• W2134711862 date "2011-11-01" @default.
• W2134711862 modified "2023-10-11" @default.
• W2134711862 title "Substrate channeling and enzyme complexes for biotechnological applications" @default.
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