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- W2134744211 abstract "Abstract Background Microarray co-expression signatures are an important tool for studying gene function and relations between genes. In addition to genuine biological co-expression, correlated signals can result from technical deficiencies like hybridization of reporters with off-target transcripts. An approach that is able to distinguish these factors permits the detection of more biologically relevant co-expression signatures. Results We demonstrate a positive relation between off-target reporter alignment strength and expression correlation in data from oligonucleotide genechips. Furthermore, we describe a method that allows the identification, from their expression data, of individual probe sets affected by off-target hybridization. Conclusion The effects of off-target hybridization on expression correlation coefficients can be substantial, and can be alleviated by more accurate mapping between microarray reporters and the target transcriptome. We recommend attention to the mapping for any microarray analysis of gene expression patterns." @default.
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- W2134744211 date "2007-11-26" @default.
- W2134744211 modified "2023-10-10" @default.
- W2134744211 title "In situ analysis of cross-hybridisation on microarrays and the inference of expression correlation" @default.
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- W2134744211 doi "https://doi.org/10.1186/1471-2105-8-461" @default.
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