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- W2134768344 abstract "Freeze frame: The ubiquitous ion pump, Na,K-ATPase (NKA), was discovered over half a century ago, yet atomic details of the native substrate ATP in the nucleotide site remain unavailable. Here, we use low-temperature solid-state NMR spectroscopy to detect uniformly 13C-labelled ATP bound to NKA in membrane preparations. Freeze-trapping the complex prevents ATP hydrolysis and allows the detection of specific nucleotide–enzyme contacts. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article." @default.
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- W2134768344 date "2009-07-13" @default.
- W2134768344 modified "2023-10-17" @default.
- W2134768344 title "Solid-State NMR Studies of Adenosine 5′-Triphosphate Freeze-Trapped in the Nucleotide Site of Na,K-ATPase" @default.
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- W2134768344 doi "https://doi.org/10.1002/cbic.200900167" @default.
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