Matches in SemOpenAlex for { <https://semopenalex.org/work/W2134849082> ?p ?o ?g. }
- W2134849082 endingPage "6045" @default.
- W2134849082 startingPage "6040" @default.
- W2134849082 abstract "The aim of this study was to construct expression vectors carrying mouse peroxisomal protein gene (PEP-cDNA) in prokaryotic and mammalian expression vectors in chimeric cDNA types, encompassing GST and FLAG with PEP-cDNA.PEP-cDNA was sub-cloned in pGEX6p2 prokaryotic expression vector in order to label this gene with GST to purify PEP protein for further biochemical analysis and identifying related proteins thereafter. FLAG-PEP recombinant DNA was produced and sub-cloned in pUcD3 eukaryotic expression vector to express tagged-PEP protein for transient transfection analysis and identifying intracellular localization of PEP protein in future experiments. PEP-cDNA was amplified in different PCR reactions using pEGFP-PEP vector and 2 sets of primers introducing specific restriction sites at the ends of PEP. PCR products with BamHI/SalI restriction sites were treated by restriction enzymes and inserted into the pGEX6p2, downstream of GST tag. PEP-cDNA containing BamHI/ApaI restriction sites and FLAG gene (which amplified using pUcD3-FLAG-PEX3 vector) were used as templates in secondary PCR for amplifying FLAG-PEP recombinant DNA. FLAG-PEP fragment was treated by enzymatic digestion and inserted into the pUcD3 eukaryotic expression vector. pGEX6p2-PEP and pUcD3-FLAG-PEP constructed vectors were transformed into the one shot TOP10 and JM105 bacterial competent cells, respectively. Positive colonies were selected for plasmid preparation. Results confirmed correct amplification of the expected products. PEP-cDNA in both PCR reactions encompasses 630 bp. FLAG fragment containing designed sites was 77 bp and FLAG-PEP fragment was 700 bp. Sequencing of constructed vectors confirmed that PEP-cDNA was tagged appropriately and inserted free of mutation and in frame with GST and FLAG. Key word: PEP cDNA, pGEX6p2 vector, peroxisome, PTS1 signal." @default.
- W2134849082 created "2016-06-24" @default.
- W2134849082 creator A5000705981 @default.
- W2134849082 creator A5006233759 @default.
- W2134849082 creator A5009688016 @default.
- W2134849082 creator A5012850094 @default.
- W2134849082 creator A5019629281 @default.
- W2134849082 creator A5019924570 @default.
- W2134849082 creator A5022981519 @default.
- W2134849082 creator A5024530767 @default.
- W2134849082 creator A5045796424 @default.
- W2134849082 creator A5045958617 @default.
- W2134849082 creator A5080351849 @default.
- W2134849082 date "2009-11-16" @default.
- W2134849082 modified "2023-09-27" @default.
- W2134849082 title "Construction of expression vectors carrying mouse peroxisomal protein gene (PeP) with GST and Flag labels" @default.
- W2134849082 cites W1570204140 @default.
- W2134849082 cites W1583276720 @default.
- W2134849082 cites W1719904978 @default.
- W2134849082 cites W1920423370 @default.
- W2134849082 cites W1996044489 @default.
- W2134849082 cites W2003432454 @default.
- W2134849082 cites W2027647762 @default.
- W2134849082 cites W2031164640 @default.
- W2134849082 cites W2037318971 @default.
- W2134849082 cites W2049208901 @default.
- W2134849082 cites W2049234265 @default.
- W2134849082 cites W2070923978 @default.
- W2134849082 cites W2076077839 @default.
- W2134849082 cites W2077141296 @default.
- W2134849082 cites W2084514383 @default.
- W2134849082 cites W2112456573 @default.
- W2134849082 cites W2115027228 @default.
- W2134849082 cites W2116063223 @default.
- W2134849082 cites W2119598920 @default.
- W2134849082 cites W2130350654 @default.
- W2134849082 cites W2132762612 @default.
- W2134849082 cites W2146095773 @default.
- W2134849082 cites W2148766990 @default.
- W2134849082 cites W2156330912 @default.
- W2134849082 cites W2159803276 @default.
- W2134849082 cites W2162421551 @default.
- W2134849082 cites W2168101937 @default.
- W2134849082 cites W2170566884 @default.
- W2134849082 cites W2413107236 @default.
- W2134849082 doi "https://doi.org/10.5897/ajb2009.000-9492" @default.
- W2134849082 hasPublicationYear "2009" @default.
- W2134849082 type Work @default.
- W2134849082 sameAs 2134849082 @default.
- W2134849082 citedByCount "0" @default.
- W2134849082 crossrefType "journal-article" @default.
- W2134849082 hasAuthorship W2134849082A5000705981 @default.
- W2134849082 hasAuthorship W2134849082A5006233759 @default.
- W2134849082 hasAuthorship W2134849082A5009688016 @default.
- W2134849082 hasAuthorship W2134849082A5012850094 @default.
- W2134849082 hasAuthorship W2134849082A5019629281 @default.
- W2134849082 hasAuthorship W2134849082A5019924570 @default.
- W2134849082 hasAuthorship W2134849082A5022981519 @default.
- W2134849082 hasAuthorship W2134849082A5024530767 @default.
- W2134849082 hasAuthorship W2134849082A5045796424 @default.
- W2134849082 hasAuthorship W2134849082A5045958617 @default.
- W2134849082 hasAuthorship W2134849082A5080351849 @default.
- W2134849082 hasBestOaLocation W21348490821 @default.
- W2134849082 hasConcept C104317684 @default.
- W2134849082 hasConcept C128319531 @default.
- W2134849082 hasConcept C150194340 @default.
- W2134849082 hasConcept C153911025 @default.
- W2134849082 hasConcept C187882448 @default.
- W2134849082 hasConcept C33161422 @default.
- W2134849082 hasConcept C40767141 @default.
- W2134849082 hasConcept C42174275 @default.
- W2134849082 hasConcept C54355233 @default.
- W2134849082 hasConcept C58301748 @default.
- W2134849082 hasConcept C86803240 @default.
- W2134849082 hasConceptScore W2134849082C104317684 @default.
- W2134849082 hasConceptScore W2134849082C128319531 @default.
- W2134849082 hasConceptScore W2134849082C150194340 @default.
- W2134849082 hasConceptScore W2134849082C153911025 @default.
- W2134849082 hasConceptScore W2134849082C187882448 @default.
- W2134849082 hasConceptScore W2134849082C33161422 @default.
- W2134849082 hasConceptScore W2134849082C40767141 @default.
- W2134849082 hasConceptScore W2134849082C42174275 @default.
- W2134849082 hasConceptScore W2134849082C54355233 @default.
- W2134849082 hasConceptScore W2134849082C58301748 @default.
- W2134849082 hasConceptScore W2134849082C86803240 @default.
- W2134849082 hasIssue "22" @default.
- W2134849082 hasLocation W21348490821 @default.
- W2134849082 hasOpenAccess W2134849082 @default.
- W2134849082 hasPrimaryLocation W21348490821 @default.
- W2134849082 hasRelatedWork W2338137066 @default.
- W2134849082 hasRelatedWork W2347276613 @default.
- W2134849082 hasRelatedWork W2351771198 @default.
- W2134849082 hasRelatedWork W2361242162 @default.
- W2134849082 hasRelatedWork W2373690171 @default.
- W2134849082 hasRelatedWork W2382749277 @default.
- W2134849082 hasRelatedWork W2386419125 @default.
- W2134849082 hasRelatedWork W2389269354 @default.
- W2134849082 hasRelatedWork W2413210204 @default.