FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2134903517> ?p ?o ?g. }

• W2134903517 endingPage "1458" @default.
• W2134903517 startingPage "1450" @default.
• W2134903517 abstract "The serine-arginine-rich (SR) proteins constitute a conserved family of pre-mRNA splicing factors. In Arabidopsis (Arabidopsis thaliana), they are encoded by 19 genes, most of which are themselves alternatively spliced. In the case of SR45, the use of alternative 3' splice sites 21 nucleotides apart generates two alternatively spliced isoforms. Isoform 1 (SR45.1) has an insertion relative to isoform 2 (SR45.2) that replaces a single arginine with eight amino acids (TSPQRKTG). The biological implications of SR45 alternative splicing have been unclear. A previously described loss-of-function mutant affecting both isoforms, sr45-1, shows several developmental defects, including defects in petal development and root growth. We found that the SR45 promoter is highly active in regions with actively growing and dividing cells. We also tested the ability of each SR45 isoform to complement the sr45-1 mutant by overexpression of isoform-specific green fluorescent protein (GFP) fusion proteins. As expected, transgenic plants overexpressing either isoform displayed both nuclear speckles and GFP fluorescence throughout the nucleoplasm. We found that SR45.1-GFP complements the flower petal phenotype, but not the root growth phenotype. Conversely, SR45.2-GFP complements root growth but not floral morphology. Mutation of a predicted phosphorylation site within the alternatively spliced segment, SR45.1-S219A-GFP, does not affect complementation. However, a double mutation affecting both serine-219 and the adjacent threonine-218 (SR45.1-T218A + S219A-GFP) behaves like isoform 2, complementing the root but not the floral phenotype. In conclusion, our study provides evidence that the two alternatively spliced isoforms of SR45 have distinct biological functions." @default.
• W2134903517 created "2016-06-24" @default.
• W2134903517 creator A5015890789 @default.
• W2134903517 creator A5082985600 @default.
• W2134903517 date "2009-04-29" @default.
• W2134903517 modified "2023-10-07" @default.
• W2134903517 title "Two Alternatively Spliced Isoforms of the Arabidopsis SR45 Protein Have Distinct Roles during Normal Plant Development " @default.
• W2134903517 cites W1539528380 @default.
• W2134903517 cites W1563462044 @default.
• W2134903517 cites W1581360631 @default.
• W2134903517 cites W1979354217 @default.
• W2134903517 cites W2002360203 @default.
• W2134903517 cites W2002812438 @default.
• W2134903517 cites W2005592449 @default.
• W2134903517 cites W2006434152 @default.
• W2134903517 cites W2009316231 @default.
• W2134903517 cites W2018914487 @default.
• W2134903517 cites W2021033136 @default.
• W2134903517 cites W2026657651 @default.
• W2134903517 cites W2026749269 @default.
• W2134903517 cites W2027676750 @default.
• W2134903517 cites W2028507354 @default.
• W2134903517 cites W2036122702 @default.
• W2134903517 cites W2038163496 @default.
• W2134903517 cites W2038888441 @default.
• W2134903517 cites W2047710235 @default.
• W2134903517 cites W2054262102 @default.
• W2134903517 cites W2056764302 @default.
• W2134903517 cites W2057880514 @default.
• W2134903517 cites W2079384083 @default.
• W2134903517 cites W2084084328 @default.
• W2134903517 cites W2096451658 @default.
• W2134903517 cites W2097116113 @default.
• W2134903517 cites W2100899690 @default.
• W2134903517 cites W2115300775 @default.
• W2134903517 cites W2116348004 @default.
• W2134903517 cites W2120261223 @default.
• W2134903517 cites W2132300055 @default.
• W2134903517 cites W2135793062 @default.
• W2134903517 cites W2138400976 @default.
• W2134903517 cites W2145322004 @default.
• W2134903517 cites W2154649087 @default.
• W2134903517 cites W2155336429 @default.
• W2134903517 cites W2156466598 @default.
• W2134903517 cites W2157780840 @default.
• W2134903517 cites W2159864549 @default.
• W2134903517 cites W2160391145 @default.
• W2134903517 cites W2162981291 @default.
• W2134903517 cites W2165535624 @default.
• W2134903517 doi "https://doi.org/10.1104/pp.109.138180" @default.
• W2134903517 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2705014" @default.
• W2134903517 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19403727" @default.
• W2134903517 hasPublicationYear "2009" @default.
• W2134903517 type Work @default.
• W2134903517 sameAs 2134903517 @default.
• W2134903517 citedByCount "130" @default.
• W2134903517 countsByYear W21349035172012 @default.
• W2134903517 countsByYear W21349035172013 @default.
• W2134903517 countsByYear W21349035172014 @default.
• W2134903517 countsByYear W21349035172015 @default.
• W2134903517 countsByYear W21349035172016 @default.
• W2134903517 countsByYear W21349035172017 @default.
• W2134903517 countsByYear W21349035172018 @default.
• W2134903517 countsByYear W21349035172019 @default.
• W2134903517 countsByYear W21349035172020 @default.
• W2134903517 countsByYear W21349035172021 @default.
• W2134903517 countsByYear W21349035172022 @default.
• W2134903517 countsByYear W21349035172023 @default.
• W2134903517 crossrefType "journal-article" @default.
• W2134903517 hasAuthorship W2134903517A5015890789 @default.
• W2134903517 hasAuthorship W2134903517A5082985600 @default.
• W2134903517 hasBestOaLocation W21349035172 @default.
• W2134903517 hasConcept C104317684 @default.
• W2134903517 hasConcept C123243835 @default.
• W2134903517 hasConcept C127716648 @default.
• W2134903517 hasConcept C142613039 @default.
• W2134903517 hasConcept C143065580 @default.
• W2134903517 hasConcept C153911025 @default.
• W2134903517 hasConcept C188082640 @default.
• W2134903517 hasConcept C194583182 @default.
• W2134903517 hasConcept C2775988993 @default.
• W2134903517 hasConcept C2779491563 @default.
• W2134903517 hasConcept C53345823 @default.
• W2134903517 hasConcept C54355233 @default.
• W2134903517 hasConcept C54458228 @default.
• W2134903517 hasConcept C67705224 @default.
• W2134903517 hasConcept C86803240 @default.
• W2134903517 hasConcept C95444343 @default.
• W2134903517 hasConceptScore W2134903517C104317684 @default.
• W2134903517 hasConceptScore W2134903517C123243835 @default.
• W2134903517 hasConceptScore W2134903517C127716648 @default.
• W2134903517 hasConceptScore W2134903517C142613039 @default.
• W2134903517 hasConceptScore W2134903517C143065580 @default.
• W2134903517 hasConceptScore W2134903517C153911025 @default.
• W2134903517 hasConceptScore W2134903517C188082640 @default.
• W2134903517 hasConceptScore W2134903517C194583182 @default.
• W2134903517 hasConceptScore W2134903517C2775988993 @default.
• W2134903517 hasConceptScore W2134903517C2779491563 @default.

• next