FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2134968390> ?p ?o ?g. }

• W2134968390 endingPage "532" @default.
• W2134968390 startingPage "522" @default.
• W2134968390 abstract "To take advantage of the growing knowledge of cellular signaling pathways, modern-day drug discovery faces an increasing challenge to develop assays to screen for compounds that modulate protein-protein interactions. One bottleneck in achieving this goal is a lack of suitable and robust assay technologies amenable to a high-throughput format. In this report, we describe how we utilized Alphascreen trade mark technology to develop a high-throughput assay to monitor ligand binding to a member of the tumor necrosis factor receptor superfamily. We expressed a fusion protein consisting of the extracellular domain of the OX40 receptor with the constant domains of human IgG. In the presence of OX40 ligand, we determined a binding affinity constant consistent with reported values and optimized the protocol to develop a simple, homogeneous, and sensitive binding assay in a 384-well format. Finally, we assessed if this system could identify small peptides capable of inhibiting the OX40 receptor and ligand interaction. The results showed that the assay was able to detect such peptides and could be used to launch a high-throughput screening campaign for small molecules able to prevent OX40 receptor activation." @default.
• W2134968390 created "2016-06-24" @default.
• W2134968390 creator A5023891526 @default.
• W2134968390 creator A5042568915 @default.
• W2134968390 creator A5042753215 @default.
• W2134968390 creator A5049341325 @default.
• W2134968390 creator A5056840112 @default.
• W2134968390 creator A5085647135 @default.
• W2134968390 creator A5085706199 @default.
• W2134968390 creator A5089194350 @default.
• W2134968390 date "2003-10-01" @default.
• W2134968390 modified "2023-10-16" @default.
• W2134968390 title "A Homogeneous 384-Well High-Throughput Binding Assay for a TNF Receptor Using Alphascreen Technology" @default.
• W2134968390 cites W1556719010 @default.
• W2134968390 cites W1907719511 @default.
• W2134968390 cites W1936218468 @default.
• W2134968390 cites W1974428835 @default.
• W2134968390 cites W1980629165 @default.
• W2134968390 cites W1981239780 @default.
• W2134968390 cites W1990345548 @default.
• W2134968390 cites W1991001144 @default.
• W2134968390 cites W1991639533 @default.
• W2134968390 cites W2010300630 @default.
• W2134968390 cites W2025187365 @default.
• W2134968390 cites W2028809209 @default.
• W2134968390 cites W2033526561 @default.
• W2134968390 cites W2034801992 @default.
• W2134968390 cites W2055992184 @default.
• W2134968390 cites W2061104342 @default.
• W2134968390 cites W2063480372 @default.
• W2134968390 cites W2067230067 @default.
• W2134968390 cites W2074631079 @default.
• W2134968390 cites W2076089746 @default.
• W2134968390 cites W2079166648 @default.
• W2134968390 cites W2079694858 @default.
• W2134968390 cites W2080106148 @default.
• W2134968390 cites W2089932939 @default.
• W2134968390 cites W2123061071 @default.
• W2134968390 cites W2125553667 @default.
• W2134968390 cites W2126102214 @default.
• W2134968390 cites W2131192618 @default.
• W2134968390 cites W2140478082 @default.
• W2134968390 cites W2155292823 @default.
• W2134968390 cites W2166280560 @default.
• W2134968390 cites W2204690181 @default.
• W2134968390 cites W259249516 @default.
• W2134968390 cites W4255061500 @default.
• W2134968390 doi "https://doi.org/10.1177/1087057103257804" @default.
• W2134968390 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14567779" @default.
• W2134968390 hasPublicationYear "2003" @default.
• W2134968390 type Work @default.
• W2134968390 sameAs 2134968390 @default.
• W2134968390 citedByCount "25" @default.
• W2134968390 countsByYear W21349683902012 @default.
• W2134968390 countsByYear W21349683902013 @default.
• W2134968390 countsByYear W21349683902014 @default.
• W2134968390 countsByYear W21349683902018 @default.
• W2134968390 countsByYear W21349683902023 @default.
• W2134968390 crossrefType "journal-article" @default.
• W2134968390 hasAuthorship W2134968390A5023891526 @default.
• W2134968390 hasAuthorship W2134968390A5042568915 @default.
• W2134968390 hasAuthorship W2134968390A5042753215 @default.
• W2134968390 hasAuthorship W2134968390A5049341325 @default.
• W2134968390 hasAuthorship W2134968390A5056840112 @default.
• W2134968390 hasAuthorship W2134968390A5085647135 @default.
• W2134968390 hasAuthorship W2134968390A5085706199 @default.
• W2134968390 hasAuthorship W2134968390A5089194350 @default.
• W2134968390 hasBestOaLocation W21349683901 @default.
• W2134968390 hasConcept C104317684 @default.
• W2134968390 hasConcept C116569031 @default.
• W2134968390 hasConcept C123894998 @default.
• W2134968390 hasConcept C149635348 @default.
• W2134968390 hasConcept C161624437 @default.
• W2134968390 hasConcept C170493617 @default.
• W2134968390 hasConcept C2780513914 @default.
• W2134968390 hasConcept C40767141 @default.
• W2134968390 hasConcept C41008148 @default.
• W2134968390 hasConcept C51323132 @default.
• W2134968390 hasConcept C55493867 @default.
• W2134968390 hasConcept C70721500 @default.
• W2134968390 hasConcept C74187038 @default.
• W2134968390 hasConcept C85196483 @default.
• W2134968390 hasConcept C86803240 @default.
• W2134968390 hasConcept C95444343 @default.
• W2134968390 hasConceptScore W2134968390C104317684 @default.
• W2134968390 hasConceptScore W2134968390C116569031 @default.
• W2134968390 hasConceptScore W2134968390C123894998 @default.
• W2134968390 hasConceptScore W2134968390C149635348 @default.
• W2134968390 hasConceptScore W2134968390C161624437 @default.
• W2134968390 hasConceptScore W2134968390C170493617 @default.
• W2134968390 hasConceptScore W2134968390C2780513914 @default.
• W2134968390 hasConceptScore W2134968390C40767141 @default.
• W2134968390 hasConceptScore W2134968390C41008148 @default.
• W2134968390 hasConceptScore W2134968390C51323132 @default.
• W2134968390 hasConceptScore W2134968390C55493867 @default.
• W2134968390 hasConceptScore W2134968390C70721500 @default.
• W2134968390 hasConceptScore W2134968390C74187038 @default.
• W2134968390 hasConceptScore W2134968390C85196483 @default.

• next