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• W2134976145 abstract "The migratory locust is one of the most destructive agricultural pests worldwide. ATP synthase (F0 F1 -ATPase) uses proton or sodium motive force to produce 90% of the cellular ATP, and the α-subunit of F1 -ATP synthase (ATP5A) is vital for F1 -ATP synthase. Here, we tested whether ATP5A could be a potential target for RNAi-mediated pest management of L. migratoria.Lm-ATP5A was cloned and characterised. Lm-ATP5A is expressed in all tissues. Injection of 100 ng of the double-stranded RNA of ATP5A (dsATP5A) knocked down the transcription of the target gene and caused mortality in 1.5-5 days. The Lm-ATP5A protein level, the oligomycin-sensitive ATP synthetic and hydrolytic activities and the ATP content were correspondingly reduced following dsATP5A injection.These findings demonstrated the essential roles of Lm-ATP5A in L. migratoria and identified it as a potential target for insect pest control. © 2015 Society of Chemical Industry." @default.
• W2134976145 created "2016-06-24" @default.
• W2134976145 creator A5040476342 @default.
• W2134976145 creator A5062766009 @default.
• W2134976145 date "2015-12-18" @default.
• W2134976145 modified "2023-09-23" @default.
• W2134976145 title "F₁-ATP synthase<i>α</i>-subunit: a potential target for RNAi-mediated pest management of<i>Locusta migratoria manilensis</i>" @default.
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• W2134976145 doi "https://doi.org/10.1002/ps.4185" @default.
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• W2134976145 hasPublicationYear "2015" @default.
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