Matches in SemOpenAlex for { <https://semopenalex.org/work/W2135000178> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2135000178 endingPage "pdb.prot5306" @default.
- W2135000178 startingPage "pdb.prot5306" @default.
- W2135000178 abstract "Water-soluble lipopolymers and lipopeptides are nonviral gene-transfer reagents that combine the advantages of lipids, which increase permeability of DNA through cell membranes, with the DNA-condensing and enhanced endosomal release properties of polycations. This protocol describes the synthesis and characterization of water-soluble lipopolymer/lipopeptide-DNA complexes and their use in transfection of cultured cells and injection into tumor-bearing mice. Lipopolymers are formed by conjugating cholesteryl chloroformate with the primary or secondary amines of 1800-Da branched polyethylenimine (PEI); the use of the 1800-Da PEI avoids the cytotoxicity problems associated with higher molecular-mass PEI. Lipopeptides are composed of a human protamine-derived peptide that has been incubated with a reaction of O-(N-succinimidyl)-N,N,N',N',-tetramethyluronium tetrafluoroborate with lithocholic acid in the presence of excess diisopropylethylamine. The therapeutic gene used as an example in this protocol encodes the murine interleukin 12 (IL-12) subunits p35 and p40, each under the transcriptional control of a separate cytomegalovirus (CMV) promoter; the reporter plasmid contains the luciferase gene driven by a CMV promoter. The target cells are C-26 colon carcinoma cells." @default.
- W2135000178 created "2016-06-24" @default.
- W2135000178 creator A5051052380 @default.
- W2135000178 creator A5056355344 @default.
- W2135000178 creator A5078667650 @default.
- W2135000178 date "2009-10-01" @default.
- W2135000178 modified "2023-09-25" @default.
- W2135000178 title "Formation and Use of Water-Soluble Lipopolymer-pDNA and Lipopeptide-pDNA Complexes" @default.
- W2135000178 cites W1973350289 @default.
- W2135000178 cites W2083100535 @default.
- W2135000178 cites W2083851430 @default.
- W2135000178 cites W1526171851 @default.
- W2135000178 cites W2938408720 @default.
- W2135000178 doi "https://doi.org/10.1101/pdb.prot5306" @default.
- W2135000178 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20147051" @default.
- W2135000178 hasPublicationYear "2009" @default.
- W2135000178 type Work @default.
- W2135000178 sameAs 2135000178 @default.
- W2135000178 citedByCount "0" @default.
- W2135000178 crossrefType "journal-article" @default.
- W2135000178 hasAuthorship W2135000178A5051052380 @default.
- W2135000178 hasAuthorship W2135000178A5056355344 @default.
- W2135000178 hasAuthorship W2135000178A5078667650 @default.
- W2135000178 hasConcept C104317684 @default.
- W2135000178 hasConcept C109316439 @default.
- W2135000178 hasConcept C135983454 @default.
- W2135000178 hasConcept C150194340 @default.
- W2135000178 hasConcept C153911025 @default.
- W2135000178 hasConcept C161733203 @default.
- W2135000178 hasConcept C185592680 @default.
- W2135000178 hasConcept C202751555 @default.
- W2135000178 hasConcept C2775925850 @default.
- W2135000178 hasConcept C2777425210 @default.
- W2135000178 hasConcept C2779399885 @default.
- W2135000178 hasConcept C2780420665 @default.
- W2135000178 hasConcept C523546767 @default.
- W2135000178 hasConcept C54009773 @default.
- W2135000178 hasConcept C54355233 @default.
- W2135000178 hasConcept C552990157 @default.
- W2135000178 hasConcept C55493867 @default.
- W2135000178 hasConcept C86803240 @default.
- W2135000178 hasConceptScore W2135000178C104317684 @default.
- W2135000178 hasConceptScore W2135000178C109316439 @default.
- W2135000178 hasConceptScore W2135000178C135983454 @default.
- W2135000178 hasConceptScore W2135000178C150194340 @default.
- W2135000178 hasConceptScore W2135000178C153911025 @default.
- W2135000178 hasConceptScore W2135000178C161733203 @default.
- W2135000178 hasConceptScore W2135000178C185592680 @default.
- W2135000178 hasConceptScore W2135000178C202751555 @default.
- W2135000178 hasConceptScore W2135000178C2775925850 @default.
- W2135000178 hasConceptScore W2135000178C2777425210 @default.
- W2135000178 hasConceptScore W2135000178C2779399885 @default.
- W2135000178 hasConceptScore W2135000178C2780420665 @default.
- W2135000178 hasConceptScore W2135000178C523546767 @default.
- W2135000178 hasConceptScore W2135000178C54009773 @default.
- W2135000178 hasConceptScore W2135000178C54355233 @default.
- W2135000178 hasConceptScore W2135000178C552990157 @default.
- W2135000178 hasConceptScore W2135000178C55493867 @default.
- W2135000178 hasConceptScore W2135000178C86803240 @default.
- W2135000178 hasIssue "10" @default.
- W2135000178 hasLocation W21350001781 @default.
- W2135000178 hasLocation W21350001782 @default.
- W2135000178 hasOpenAccess W2135000178 @default.
- W2135000178 hasPrimaryLocation W21350001781 @default.
- W2135000178 hasRelatedWork W2073678610 @default.
- W2135000178 hasRelatedWork W2102539196 @default.
- W2135000178 hasRelatedWork W2109584932 @default.
- W2135000178 hasRelatedWork W2135000178 @default.
- W2135000178 hasRelatedWork W2373663690 @default.
- W2135000178 hasRelatedWork W2377592610 @default.
- W2135000178 hasRelatedWork W2586189433 @default.
- W2135000178 hasRelatedWork W2959800051 @default.
- W2135000178 hasRelatedWork W3150187863 @default.
- W2135000178 hasRelatedWork W4225124523 @default.
- W2135000178 hasVolume "2009" @default.
- W2135000178 isParatext "false" @default.
- W2135000178 isRetracted "false" @default.
- W2135000178 magId "2135000178" @default.
- W2135000178 workType "article" @default.