FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2135032014> ?p ?o ?g. }

• W2135032014 endingPage "603" @default.
• W2135032014 startingPage "583" @default.
• W2135032014 abstract "The InsP3R Ca2+ release channel has a biphasic dependence on cytoplasmic free Ca2+ concentration ([Ca2+]i). InsP3 activates gating primarily by reducing the sensitivity of the channel to inhibition by high [Ca2+]i. To determine if relieving Ca2+ inhibition is sufficient for channel activation, we examined single-channel activities in low [Ca2+]i in the absence of InsP3, by patch clamping isolated Xenopus oocyte nuclei. For both endogenous Xenopus type 1 and recombinant rat type 3 InsP3R channels, spontaneous InsP3-independent channel activities with low open probability Po (∼0.03) were observed in [Ca2+]i &lt; 5 nM with the same frequency as in the presence of InsP3, whereas no activities were observed in 25 nM Ca2+. These results establish the half-maximal inhibitory [Ca2+]i of the channel to be 1.2–4.0 nM in the absence of InsP3, and demonstrate that the channel can be active when all of its ligand-binding sites (including InsP3) are unoccupied. In the simplest allosteric model that fits all observations in nuclear patch-clamp studies of [Ca2+]i and InsP3 regulation of steady-state channel gating behavior of types 1 and 3 InsP3R isoforms, including spontaneous InsP3-independent channel activities, the tetrameric channel can adopt six different conformations, the equilibria among which are controlled by two inhibitory and one activating Ca2+-binding and one InsP3-binding sites in a manner outlined in the Monod-Wyman-Changeux model. InsP3 binding activates gating by affecting the Ca2+ affinities of the high-affinity inhibitory sites in different conformations, transforming it into an activating site. Ca2+ inhibition of InsP3-liganded channels is mediated by an InsP3-independent low-affinity inhibitory site. The model also suggests that besides the ligand-regulated gating mechanism, the channel has a ligand-independent gating mechanism responsible for maximum channel Po being less than unity. The validity of this model was established by its successful quantitative prediction of channel behavior after it had been exposed to ultra-low bath [Ca2+]." @default.
• W2135032014 created "2016-06-24" @default.
• W2135032014 creator A5030221375 @default.
• W2135032014 creator A5059027982 @default.
• W2135032014 creator A5071760145 @default.
• W2135032014 date "2003-10-27" @default.
• W2135032014 modified "2023-09-24" @default.
• W2135032014 title "Spontaneous Channel Activity of the Inositol 1,4,5-Trisphosphate (InsP3) Receptor (InsP3R). Application of Allosteric Modeling to Calcium and InsP3 Regulation of InsP3R Single-channel Gating" @default.
• W2135032014 cites W1502625840 @default.
• W2135032014 cites W1523078822 @default.
• W2135032014 cites W1788868849 @default.
• W2135032014 cites W1929385394 @default.
• W2135032014 cites W1972994417 @default.
• W2135032014 cites W1973276470 @default.
• W2135032014 cites W1973646870 @default.
• W2135032014 cites W1977810278 @default.
• W2135032014 cites W1979165244 @default.
• W2135032014 cites W1980166477 @default.
• W2135032014 cites W1997778815 @default.
• W2135032014 cites W1998093640 @default.
• W2135032014 cites W2003962250 @default.
• W2135032014 cites W2011257591 @default.
• W2135032014 cites W2014183428 @default.
• W2135032014 cites W2020828897 @default.
• W2135032014 cites W2021682147 @default.
• W2135032014 cites W2021847777 @default.
• W2135032014 cites W2038780532 @default.
• W2135032014 cites W2045399399 @default.
• W2135032014 cites W2048682543 @default.
• W2135032014 cites W2054753751 @default.
• W2135032014 cites W2058298535 @default.
• W2135032014 cites W2060891098 @default.
• W2135032014 cites W2061875631 @default.
• W2135032014 cites W2070253406 @default.
• W2135032014 cites W2077640402 @default.
• W2135032014 cites W2091358267 @default.
• W2135032014 cites W2095909835 @default.
• W2135032014 cites W2099430463 @default.
• W2135032014 cites W2100265629 @default.
• W2135032014 cites W2108411628 @default.
• W2135032014 cites W2111748376 @default.
• W2135032014 cites W2126601560 @default.
• W2135032014 cites W2135032014 @default.
• W2135032014 cites W2146387077 @default.
• W2135032014 cites W2155044464 @default.
• W2135032014 cites W2155885957 @default.
• W2135032014 cites W2157844576 @default.
• W2135032014 cites W2160385558 @default.
• W2135032014 cites W2163248783 @default.
• W2135032014 cites W2170006809 @default.
• W2135032014 cites W2170688389 @default.
• W2135032014 cites W2171437709 @default.
• W2135032014 cites W4249299354 @default.
• W2135032014 doi "https://doi.org/10.1085/jgp.200308809" @default.
• W2135032014 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2229577" @default.
• W2135032014 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14581584" @default.
• W2135032014 hasPublicationYear "2003" @default.
• W2135032014 type Work @default.
• W2135032014 sameAs 2135032014 @default.
• W2135032014 citedByCount "54" @default.
• W2135032014 countsByYear W21350320142012 @default.
• W2135032014 countsByYear W21350320142013 @default.
• W2135032014 countsByYear W21350320142014 @default.
• W2135032014 countsByYear W21350320142015 @default.
• W2135032014 countsByYear W21350320142016 @default.
• W2135032014 countsByYear W21350320142017 @default.
• W2135032014 countsByYear W21350320142018 @default.
• W2135032014 countsByYear W21350320142019 @default.
• W2135032014 countsByYear W21350320142022 @default.
• W2135032014 crossrefType "journal-article" @default.
• W2135032014 hasAuthorship W2135032014A5030221375 @default.
• W2135032014 hasAuthorship W2135032014A5059027982 @default.
• W2135032014 hasAuthorship W2135032014A5071760145 @default.
• W2135032014 hasBestOaLocation W21350320141 @default.
• W2135032014 hasConcept C104317684 @default.
• W2135032014 hasConcept C107824862 @default.
• W2135032014 hasConcept C12554922 @default.
• W2135032014 hasConcept C147944092 @default.
• W2135032014 hasConcept C166342909 @default.
• W2135032014 hasConcept C170493617 @default.
• W2135032014 hasConcept C178790620 @default.
• W2135032014 hasConcept C185592680 @default.
• W2135032014 hasConcept C194544171 @default.
• W2135032014 hasConcept C2777427919 @default.
• W2135032014 hasConcept C2779535977 @default.
• W2135032014 hasConcept C30979828 @default.
• W2135032014 hasConcept C519063684 @default.
• W2135032014 hasConcept C55493867 @default.
• W2135032014 hasConcept C85520022 @default.
• W2135032014 hasConcept C86803240 @default.
• W2135032014 hasConcept C92645518 @default.
• W2135032014 hasConceptScore W2135032014C104317684 @default.
• W2135032014 hasConceptScore W2135032014C107824862 @default.
• W2135032014 hasConceptScore W2135032014C12554922 @default.
• W2135032014 hasConceptScore W2135032014C147944092 @default.
• W2135032014 hasConceptScore W2135032014C166342909 @default.
• W2135032014 hasConceptScore W2135032014C170493617 @default.
• W2135032014 hasConceptScore W2135032014C178790620 @default.

• next