Matches in SemOpenAlex for { <https://semopenalex.org/work/W2135227559> ?p ?o ?g. }
- W2135227559 endingPage "7265" @default.
- W2135227559 startingPage "7256" @default.
- W2135227559 abstract "The tRNA of the miaB2508::Tn10dCm mutant of Salmonella typhimurium is deficient in the methylthio group of the modified nucleoside N(6)-(4-hydroxyisopentenyl)-2-methylthioadenosine (ms(2)io(6)A37). By sequencing, we found that the Tn10dCm of this strain had been inserted into the f474 (yleA) open reading frame, which is located close to the nag locus in both S. typhimurium and Escherichia coli. By complementation of the miaB2508::Tn10dCm mutation with a minimal subcloned f474 fragment, we showed that f474 could be identified as the miaB gene, which is transcribed in the counterclockwise direction on the bacterial chromosome. Transcriptional studies revealed two promoters upstream of miaB in E. coli and S. typhimurium. A Rho-independent terminator was identified downstream of the miaB gene, at which the majority (96%) of the miaB transcripts terminate in E. coli, showing that the miaB gene is part of a monocistronic operon. A highly conserved motif with three cysteine residues was present in MiaB. This motif resembles iron-binding sites in other proteins. Only a weak similarity to an AdoMet-binding site was found, favoring the idea that the MiaB protein is involved in the thiolation step and not in the methylating reaction of ms(2)i(o)(6)A37 formation." @default.
- W2135227559 created "2016-06-24" @default.
- W2135227559 creator A5003577027 @default.
- W2135227559 creator A5006090738 @default.
- W2135227559 creator A5057847229 @default.
- W2135227559 creator A5085302066 @default.
- W2135227559 creator A5090225020 @default.
- W2135227559 date "1999-12-01" @default.
- W2135227559 modified "2023-10-15" @default.
- W2135227559 title "Identification of the <i>miaB</i> Gene, Involved in Methylthiolation of Isopentenylated A37 Derivatives in the tRNA of <i>Salmonella typhimurium</i> and <i>Escherichia coli</i>" @default.
- W2135227559 cites W141649614 @default.
- W2135227559 cites W1480557394 @default.
- W2135227559 cites W148263968 @default.
- W2135227559 cites W1486946238 @default.
- W2135227559 cites W1541196086 @default.
- W2135227559 cites W1579016877 @default.
- W2135227559 cites W1673573808 @default.
- W2135227559 cites W1838863859 @default.
- W2135227559 cites W1840854609 @default.
- W2135227559 cites W1850384628 @default.
- W2135227559 cites W1894856070 @default.
- W2135227559 cites W1920218959 @default.
- W2135227559 cites W1940525454 @default.
- W2135227559 cites W1956228355 @default.
- W2135227559 cites W1967485807 @default.
- W2135227559 cites W1972671967 @default.
- W2135227559 cites W1974016379 @default.
- W2135227559 cites W1974749723 @default.
- W2135227559 cites W1976497970 @default.
- W2135227559 cites W1986738890 @default.
- W2135227559 cites W1992648037 @default.
- W2135227559 cites W1997125979 @default.
- W2135227559 cites W2000290983 @default.
- W2135227559 cites W2001955359 @default.
- W2135227559 cites W2011057627 @default.
- W2135227559 cites W2011858564 @default.
- W2135227559 cites W2013095186 @default.
- W2135227559 cites W2014564496 @default.
- W2135227559 cites W2016544300 @default.
- W2135227559 cites W2022002419 @default.
- W2135227559 cites W2033741984 @default.
- W2135227559 cites W2038356555 @default.
- W2135227559 cites W2040276985 @default.
- W2135227559 cites W2042412127 @default.
- W2135227559 cites W2045991415 @default.
- W2135227559 cites W2046543676 @default.
- W2135227559 cites W2047119353 @default.
- W2135227559 cites W2051202084 @default.
- W2135227559 cites W2058772940 @default.
- W2135227559 cites W2063450941 @default.
- W2135227559 cites W2076932041 @default.
- W2135227559 cites W2093810909 @default.
- W2135227559 cites W2095716115 @default.
- W2135227559 cites W2100773761 @default.
- W2135227559 cites W2114025420 @default.
- W2135227559 cites W2117019496 @default.
- W2135227559 cites W2117650481 @default.
- W2135227559 cites W2131282911 @default.
- W2135227559 cites W2134153749 @default.
- W2135227559 cites W2147187494 @default.
- W2135227559 cites W2149913454 @default.
- W2135227559 cites W2152761700 @default.
- W2135227559 cites W2158714788 @default.
- W2135227559 cites W2165520343 @default.
- W2135227559 cites W2166574146 @default.
- W2135227559 cites W2317914227 @default.
- W2135227559 cites W2463267938 @default.
- W2135227559 cites W4296765338 @default.
- W2135227559 cites W50271218 @default.
- W2135227559 cites W962685969 @default.
- W2135227559 doi "https://doi.org/10.1128/jb.181.23.7256-7265.1999" @default.
- W2135227559 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/103688" @default.
- W2135227559 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10572129" @default.
- W2135227559 hasPublicationYear "1999" @default.
- W2135227559 type Work @default.
- W2135227559 sameAs 2135227559 @default.
- W2135227559 citedByCount "101" @default.
- W2135227559 countsByYear W21352275592012 @default.
- W2135227559 countsByYear W21352275592013 @default.
- W2135227559 countsByYear W21352275592014 @default.
- W2135227559 countsByYear W21352275592015 @default.
- W2135227559 countsByYear W21352275592016 @default.
- W2135227559 countsByYear W21352275592017 @default.
- W2135227559 countsByYear W21352275592018 @default.
- W2135227559 countsByYear W21352275592019 @default.
- W2135227559 countsByYear W21352275592020 @default.
- W2135227559 countsByYear W21352275592021 @default.
- W2135227559 countsByYear W21352275592022 @default.
- W2135227559 countsByYear W21352275592023 @default.
- W2135227559 crossrefType "journal-article" @default.
- W2135227559 hasAuthorship W2135227559A5003577027 @default.
- W2135227559 hasAuthorship W2135227559A5006090738 @default.
- W2135227559 hasAuthorship W2135227559A5057847229 @default.
- W2135227559 hasAuthorship W2135227559A5085302066 @default.
- W2135227559 hasAuthorship W2135227559A5090225020 @default.
- W2135227559 hasBestOaLocation W21352275591 @default.
- W2135227559 hasConcept C104317684 @default.
- W2135227559 hasConcept C116403925 @default.