FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2135249375> ?p ?o ?g. }

• W2135249375 endingPage "11133" @default.
• W2135249375 startingPage "11124" @default.
• W2135249375 abstract "ABSTRACT Resistance to hepatitis C virus (HCV) inhibitors targeting viral enzymes has been observed in in vitro replicon studies and during clinical trials. The factors determining the emergence of resistance and the changes in the viral quasispecies population under selective pressure are not fully understood. To assess the dynamics of variants emerging in vitro under various selective pressures with TMC380765, a potent macrocyclic HCV NS3/4A protease inhibitor, HCV genotype 1b replicon-containing cells were cultured in the presence of a low, high, or stepwise-increasing TMC380765 concentration(s). HCV replicon RNA from representative samples thus obtained was analyzed using (i) population, (ii) clonal, and (iii) 454 deep sequencing technologies. Depending on the concentration of TMC380765, distinct mutational patterns emerged. In particular, culturing with low concentrations resulted in the selection of low-level resistance mutations (F43S and A156G), whereas high concentrations resulted in the selection of high-level resistance mutations (A156V, D168V, and D168A). Clonal and 454 deep sequencing analysis of the replicon RNA allowed the identification of low-frequency preexisting mutations possibly contributing to the mutational pattern that emerged. Stepwise-increasing TMC380765 concentrations resulted in the emergence and disappearance of multiple replicon variants in response to the changing selection pressure. Moreover, two different codons for the wild-type amino acids were observed at certain NS3 positions within one population of replicons, which may contribute to the emerging mutational patterns. Deep sequencing technologies enabled the study of minority variants present in the HCV quasispecies population present at baseline and during antiviral drug pressure, giving new insights into the dynamics of resistance acquisition by HCV." @default.
• W2135249375 created "2016-06-24" @default.
• W2135249375 creator A5002133419 @default.
• W2135249375 creator A5003454997 @default.
• W2135249375 creator A5024113787 @default.
• W2135249375 creator A5025711142 @default.
• W2135249375 creator A5027321246 @default.
• W2135249375 creator A5031660163 @default.
• W2135249375 creator A5046409769 @default.
• W2135249375 creator A5047207289 @default.
• W2135249375 creator A5074341074 @default.
• W2135249375 creator A5089010495 @default.
• W2135249375 date "2010-11-01" @default.
• W2135249375 modified "2023-09-30" @default.
• W2135249375 title "Tracking the Evolution of Multiple<i>In Vitro</i>Hepatitis C Virus Replicon Variants under Protease Inhibitor Selection Pressure by 454 Deep Sequencing" @default.
• W2135249375 cites W147241556 @default.
• W2135249375 cites W1964963193 @default.
• W2135249375 cites W1966063036 @default.
• W2135249375 cites W1973597378 @default.
• W2135249375 cites W1977935857 @default.
• W2135249375 cites W1982249405 @default.
• W2135249375 cites W1989062156 @default.
• W2135249375 cites W1989062739 @default.
• W2135249375 cites W1995565071 @default.
• W2135249375 cites W2001129358 @default.
• W2135249375 cites W2003574450 @default.
• W2135249375 cites W2007987493 @default.
• W2135249375 cites W2019523835 @default.
• W2135249375 cites W2020939898 @default.
• W2135249375 cites W2024726373 @default.
• W2135249375 cites W2029652301 @default.
• W2135249375 cites W2029777156 @default.
• W2135249375 cites W2035522972 @default.
• W2135249375 cites W2043519223 @default.
• W2135249375 cites W2046533694 @default.
• W2135249375 cites W2053485968 @default.
• W2135249375 cites W2057075704 @default.
• W2135249375 cites W2058047903 @default.
• W2135249375 cites W2058079473 @default.
• W2135249375 cites W2064569884 @default.
• W2135249375 cites W2067391188 @default.
• W2135249375 cites W2071108919 @default.
• W2135249375 cites W2072029488 @default.
• W2135249375 cites W2076654348 @default.
• W2135249375 cites W2098740488 @default.
• W2135249375 cites W2113154128 @default.
• W2135249375 cites W2120638695 @default.
• W2135249375 cites W2122589684 @default.
• W2135249375 cites W2129870123 @default.
• W2135249375 cites W2132766211 @default.
• W2135249375 cites W2140070803 @default.
• W2135249375 cites W2146397496 @default.
• W2135249375 cites W2148399664 @default.
• W2135249375 cites W2148645779 @default.
• W2135249375 cites W2151898297 @default.
• W2135249375 cites W2152695234 @default.
• W2135249375 cites W2155448001 @default.
• W2135249375 cites W2156027719 @default.
• W2135249375 cites W2158177781 @default.
• W2135249375 cites W2159835094 @default.
• W2135249375 cites W2164762627 @default.
• W2135249375 cites W2168068623 @default.
• W2135249375 cites W2170033574 @default.
• W2135249375 cites W2171002988 @default.
• W2135249375 cites W2316470888 @default.
• W2135249375 cites W2621423376 @default.
• W2135249375 doi "https://doi.org/10.1128/jvi.01217-10" @default.
• W2135249375 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2953167" @default.
• W2135249375 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20739521" @default.
• W2135249375 hasPublicationYear "2010" @default.
• W2135249375 type Work @default.
• W2135249375 sameAs 2135249375 @default.
• W2135249375 citedByCount "68" @default.
• W2135249375 countsByYear W21352493752012 @default.
• W2135249375 countsByYear W21352493752013 @default.
• W2135249375 countsByYear W21352493752014 @default.
• W2135249375 countsByYear W21352493752015 @default.
• W2135249375 countsByYear W21352493752016 @default.
• W2135249375 countsByYear W21352493752017 @default.
• W2135249375 countsByYear W21352493752018 @default.
• W2135249375 countsByYear W21352493752021 @default.
• W2135249375 countsByYear W21352493752023 @default.
• W2135249375 crossrefType "journal-article" @default.
• W2135249375 hasAuthorship W2135249375A5002133419 @default.
• W2135249375 hasAuthorship W2135249375A5003454997 @default.
• W2135249375 hasAuthorship W2135249375A5024113787 @default.
• W2135249375 hasAuthorship W2135249375A5025711142 @default.
• W2135249375 hasAuthorship W2135249375A5027321246 @default.
• W2135249375 hasAuthorship W2135249375A5031660163 @default.
• W2135249375 hasAuthorship W2135249375A5046409769 @default.
• W2135249375 hasAuthorship W2135249375A5047207289 @default.
• W2135249375 hasAuthorship W2135249375A5074341074 @default.
• W2135249375 hasAuthorship W2135249375A5089010495 @default.
• W2135249375 hasBestOaLocation W21352493751 @default.
• W2135249375 hasConcept C104317684 @default.
• W2135249375 hasConcept C132379496 @default.
• W2135249375 hasConcept C132917006 @default.
• W2135249375 hasConcept C141231307 @default.

• next