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- W2135318926 endingPage "2833" @default.
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- W2135318926 abstract "Heterochromatin protein 1 (HP1) is a conserved nonhistone chromosomal protein with functions in euchromatin and heterochromatin. Here we investigated the diffusional behaviors of HP1 isoforms in mammalian cells. Using fluorescence correlation spectroscopy (FCS) and fluorescence recovery after photobleaching (FRAP) we found that in interphase cells most HP1 molecules (50–80%) are highly mobile (recovery halftime: t 1/2 ≈ 0.9 s; diffusion coefficient: D ≈ 0.6–0.7 μm 2 s -1 ). Twenty to 40% of HP1 molecules appear to be incorporated into stable, slow-moving oligomeric complexes (t 1/2 ≈ 10 s), and constitutive heterochromatin of all mammalian cell types analyzed contain 5–7% of very slow HP1 molecules. The amount of very slow HP1 molecules correlated with the chromatin condensation state, mounting to more than 44% in condensed chromatin of transcriptionally silent cells. During mitosis 8–14% of GFP-HP1α, but not the other isoforms, are very slow within pericentromeric heterochromatin, indicating an isoform-specific function of HP1α in heterochromatin of mitotic chromosomes. These data suggest that mobile as well as very slow populations of HP1 may function in concert to maintain a stable conformation of constitutive heterochromatin throughout the cell cycle." @default.
- W2135318926 created "2016-06-24" @default.
- W2135318926 creator A5013564230 @default.
- W2135318926 creator A5023087565 @default.
- W2135318926 creator A5034245512 @default.
- W2135318926 creator A5067139246 @default.
- W2135318926 creator A5067518378 @default.
- W2135318926 date "2004-06-01" @default.
- W2135318926 modified "2023-10-11" @default.
- W2135318926 title "High- and Low-mobility Populations of HP1 in Heterochromatin of Mammalian Cells" @default.
- W2135318926 cites W129013773 @default.
- W2135318926 cites W1473264919 @default.
- W2135318926 cites W1487669901 @default.
- W2135318926 cites W1509466986 @default.
- W2135318926 cites W1513492922 @default.
- W2135318926 cites W1526580148 @default.
- W2135318926 cites W1575224321 @default.
- W2135318926 cites W1586954894 @default.
- W2135318926 cites W1603793127 @default.
- W2135318926 cites W160853306 @default.
- W2135318926 cites W1635552303 @default.
- W2135318926 cites W1931257488 @default.
- W2135318926 cites W1964952136 @default.
- W2135318926 cites W1967294295 @default.
- W2135318926 cites W1969073916 @default.
- W2135318926 cites W1974196157 @default.
- W2135318926 cites W1975210552 @default.
- W2135318926 cites W1981277012 @default.
- W2135318926 cites W1982453928 @default.
- W2135318926 cites W1982509887 @default.
- W2135318926 cites W1985036194 @default.
- W2135318926 cites W1989307095 @default.
- W2135318926 cites W1991125055 @default.
- W2135318926 cites W1994333574 @default.
- W2135318926 cites W1998475589 @default.
- W2135318926 cites W1999284470 @default.
- W2135318926 cites W2000342325 @default.
- W2135318926 cites W2002698073 @default.
- W2135318926 cites W2006095114 @default.
- W2135318926 cites W2008264883 @default.
- W2135318926 cites W2010487837 @default.
- W2135318926 cites W2012935454 @default.
- W2135318926 cites W2014656425 @default.
- W2135318926 cites W2018420071 @default.
- W2135318926 cites W2019027463 @default.
- W2135318926 cites W2019542560 @default.
- W2135318926 cites W2021691701 @default.
- W2135318926 cites W2023670646 @default.
- W2135318926 cites W2024295388 @default.
- W2135318926 cites W2026260746 @default.
- W2135318926 cites W2029301727 @default.
- W2135318926 cites W2031087599 @default.
- W2135318926 cites W2034844603 @default.
- W2135318926 cites W2035516904 @default.
- W2135318926 cites W2035574894 @default.
- W2135318926 cites W2037875706 @default.
- W2135318926 cites W2040285686 @default.
- W2135318926 cites W2041860345 @default.
- W2135318926 cites W2048645295 @default.
- W2135318926 cites W2049846825 @default.
- W2135318926 cites W2052668971 @default.
- W2135318926 cites W2054696920 @default.
- W2135318926 cites W2059696301 @default.
- W2135318926 cites W2060863780 @default.
- W2135318926 cites W2064297857 @default.
- W2135318926 cites W2064791692 @default.
- W2135318926 cites W2067724937 @default.
- W2135318926 cites W2068237765 @default.
- W2135318926 cites W2068446924 @default.
- W2135318926 cites W2068690942 @default.
- W2135318926 cites W2069080094 @default.
- W2135318926 cites W2070122527 @default.
- W2135318926 cites W2070477402 @default.
- W2135318926 cites W2073744168 @default.
- W2135318926 cites W2078955430 @default.
- W2135318926 cites W2080442119 @default.
- W2135318926 cites W2080791829 @default.
- W2135318926 cites W2086618705 @default.
- W2135318926 cites W2086776620 @default.
- W2135318926 cites W2090333901 @default.
- W2135318926 cites W2101645956 @default.
- W2135318926 cites W2101825522 @default.
- W2135318926 cites W2106028643 @default.
- W2135318926 cites W2108797661 @default.
- W2135318926 cites W2110318729 @default.
- W2135318926 cites W2110576677 @default.
- W2135318926 cites W2110618077 @default.
- W2135318926 cites W2111217339 @default.
- W2135318926 cites W2112756669 @default.
- W2135318926 cites W2113773748 @default.
- W2135318926 cites W2115795607 @default.
- W2135318926 cites W2116435209 @default.
- W2135318926 cites W2117547778 @default.
- W2135318926 cites W2118583940 @default.
- W2135318926 cites W2118740910 @default.
- W2135318926 cites W2120752254 @default.
- W2135318926 cites W2125338577 @default.
- W2135318926 cites W2128220014 @default.