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- W2135396734 endingPage "26106" @default.
- W2135396734 startingPage "26089" @default.
- W2135396734 abstract "SENSITIVE TO FREEZING 2 (SFR2) is classified as a family I glycosyl hydrolase but has recently been shown to have galactosyltransferase activity in Arabidopsis thaliana. Natural occurrences of apparent glycosyl hydrolases acting as transferases are interesting from a biocatalysis standpoint, and knowledge about the interconversion can assist in engineering SFR2 in crop plants to resist freezing. To understand how SFR2 evolved into a transferase, the relationship between its structure and function are investigated by activity assay, molecular modeling, and site-directed mutagenesis. SFR2 has no detectable hydrolase activity, although its catalytic site is highly conserved with that of family 1 glycosyl hydrolases. Three regions disparate from glycosyl hydrolases are identified as required for transferase activity as follows: a loop insertion, the C-terminal peptide, and a hydrophobic patch adjacent to the catalytic site. Rationales for the effects of these regions on the SFR2 mechanism are discussed." @default.
- W2135396734 created "2016-06-24" @default.
- W2135396734 creator A5006884142 @default.
- W2135396734 creator A5027337871 @default.
- W2135396734 creator A5029752062 @default.
- W2135396734 creator A5056977584 @default.
- W2135396734 date "2014-09-01" @default.
- W2135396734 modified "2023-10-16" @default.
- W2135396734 title "Structural Determinants Allowing Transferase Activity in SENSITIVE TO FREEZING 2, Classified as a Family I Glycosyl Hydrolase" @default.
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