Matches in SemOpenAlex for { <https://semopenalex.org/work/W2135441743> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2135441743 endingPage "446" @default.
- W2135441743 startingPage "440" @default.
- W2135441743 abstract "O-Glycans (mucin type oligosaccharides) are ubiquitously found in various glycoproteins such as mucin-type glycoproteins on the surface of various digestive organs. In the present paper, we propose a method for detecting O-glycanase which catalyzes the hydrolysis of the O-glycan linkage between oligosaccharides and serine/threonine residues of mucin-type glycoprotein. As the substrate for O-glycanases, we chose glycopeptides containing O-glycans derived from bovine fetuin. The present method is divided into two parts. At the initial stage, the presence of O-glycanase was confirmed by observing characteristic ions due to O-glycans and peptides released from the glycopeptide by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. In the second step, europium labeled O-glycosylated peptide permits more detailed analysis such as enzyme kinetics. We demonstrated the usefulness of the present method using O-glycanase (Streptococcus pneumoniae) as model enzyme. The present approach can easily confirm the presence of O-glycanase by detecting both deglycosylated peptides and O-glycans, even if contaminating peptides or glycosidases are present in crude biological samples." @default.
- W2135441743 created "2016-06-24" @default.
- W2135441743 creator A5006641864 @default.
- W2135441743 creator A5037398755 @default.
- W2135441743 creator A5060905270 @default.
- W2135441743 creator A5076864044 @default.
- W2135441743 date "2003-04-17" @default.
- W2135441743 modified "2023-09-26" @default.
- W2135441743 title "A method for detecting O-glycanase in biological samples using a combination of MALDI-TOF mass spectrometry and time-resolved fluorimetry" @default.
- W2135441743 cites W1489292103 @default.
- W2135441743 cites W1498132140 @default.
- W2135441743 cites W1949848638 @default.
- W2135441743 cites W1968834637 @default.
- W2135441743 cites W1986633830 @default.
- W2135441743 cites W2046414961 @default.
- W2135441743 cites W2058852447 @default.
- W2135441743 cites W2069949763 @default.
- W2135441743 cites W2084891151 @default.
- W2135441743 cites W2088711787 @default.
- W2135441743 cites W2096294692 @default.
- W2135441743 cites W2139368593 @default.
- W2135441743 doi "https://doi.org/10.1039/b303026a" @default.
- W2135441743 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12790194" @default.
- W2135441743 hasPublicationYear "2003" @default.
- W2135441743 type Work @default.
- W2135441743 sameAs 2135441743 @default.
- W2135441743 citedByCount "5" @default.
- W2135441743 countsByYear W21354417432014 @default.
- W2135441743 crossrefType "journal-article" @default.
- W2135441743 hasAuthorship W2135441743A5006641864 @default.
- W2135441743 hasAuthorship W2135441743A5037398755 @default.
- W2135441743 hasAuthorship W2135441743A5060905270 @default.
- W2135441743 hasAuthorship W2135441743A5076864044 @default.
- W2135441743 hasConcept C108625454 @default.
- W2135441743 hasConcept C137610392 @default.
- W2135441743 hasConcept C145148216 @default.
- W2135441743 hasConcept C150394285 @default.
- W2135441743 hasConcept C162356407 @default.
- W2135441743 hasConcept C162711632 @default.
- W2135441743 hasConcept C16291436 @default.
- W2135441743 hasConcept C178790620 @default.
- W2135441743 hasConcept C179264091 @default.
- W2135441743 hasConcept C185592680 @default.
- W2135441743 hasConcept C198291218 @default.
- W2135441743 hasConcept C206212055 @default.
- W2135441743 hasConcept C2777313579 @default.
- W2135441743 hasConcept C2778541144 @default.
- W2135441743 hasConcept C2779281246 @default.
- W2135441743 hasConcept C43617362 @default.
- W2135441743 hasConcept C501593827 @default.
- W2135441743 hasConcept C55493867 @default.
- W2135441743 hasConcept C75280812 @default.
- W2135441743 hasConceptScore W2135441743C108625454 @default.
- W2135441743 hasConceptScore W2135441743C137610392 @default.
- W2135441743 hasConceptScore W2135441743C145148216 @default.
- W2135441743 hasConceptScore W2135441743C150394285 @default.
- W2135441743 hasConceptScore W2135441743C162356407 @default.
- W2135441743 hasConceptScore W2135441743C162711632 @default.
- W2135441743 hasConceptScore W2135441743C16291436 @default.
- W2135441743 hasConceptScore W2135441743C178790620 @default.
- W2135441743 hasConceptScore W2135441743C179264091 @default.
- W2135441743 hasConceptScore W2135441743C185592680 @default.
- W2135441743 hasConceptScore W2135441743C198291218 @default.
- W2135441743 hasConceptScore W2135441743C206212055 @default.
- W2135441743 hasConceptScore W2135441743C2777313579 @default.
- W2135441743 hasConceptScore W2135441743C2778541144 @default.
- W2135441743 hasConceptScore W2135441743C2779281246 @default.
- W2135441743 hasConceptScore W2135441743C43617362 @default.
- W2135441743 hasConceptScore W2135441743C501593827 @default.
- W2135441743 hasConceptScore W2135441743C55493867 @default.
- W2135441743 hasConceptScore W2135441743C75280812 @default.
- W2135441743 hasIssue "5" @default.
- W2135441743 hasLocation W21354417431 @default.
- W2135441743 hasLocation W21354417432 @default.
- W2135441743 hasOpenAccess W2135441743 @default.
- W2135441743 hasPrimaryLocation W21354417431 @default.
- W2135441743 hasRelatedWork W154492823 @default.
- W2135441743 hasRelatedWork W1556126906 @default.
- W2135441743 hasRelatedWork W1594828236 @default.
- W2135441743 hasRelatedWork W1926815847 @default.
- W2135441743 hasRelatedWork W1970813095 @default.
- W2135441743 hasRelatedWork W1979268347 @default.
- W2135441743 hasRelatedWork W2067061290 @default.
- W2135441743 hasRelatedWork W2996444719 @default.
- W2135441743 hasRelatedWork W3034221577 @default.
- W2135441743 hasRelatedWork W2098532284 @default.
- W2135441743 hasVolume "128" @default.
- W2135441743 isParatext "false" @default.
- W2135441743 isRetracted "false" @default.
- W2135441743 magId "2135441743" @default.
- W2135441743 workType "article" @default.