FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2135525868> ?p ?o ?g. }

• W2135525868 endingPage "2090" @default.
• W2135525868 startingPage "2084" @default.
• W2135525868 abstract "Plasmids containing cDNA for the rat 67- and 65-kD isoforms of glutamate decarboxylase (GAD-67 and GAD-65) were expressed in COS-cells, and lysates of [35S]methionine-labeled cells were used for immunoprecipitations. Sera from 38 patients with type 1 (insulin-dependent) diabetes mellitus, which precipitated a 64-kD antigen from rat islets, reacted with recombinant GAD-65 in relation to their anti-64-kD titers. The eight strongest sera also precipitated recombinant GAD-67, suggesting that certain epitopes are common to both isoforms. Subsequently, [35S]methionine-labeled GAD-65 was purified from COS cell lysates and employed in a binding assay with 50 sera of patients with recent onset of type 1 diabetes mellitus. 38 sera (76%) precipitated labeled GAD-65 with titers that correlated with islet cell antibodies (ICA), determined in a standard immunofluorescence assay. 2 sera were GAD positive but ICA negative, 4 were positive only for ICA, and 6 were negative for both GAD and ICA, as were the sera of 20 controls. The data illustrate that antibodies against GAD-65 are present in a majority of patients with type 1 diabetes mellitus and that autoantibodies against other islet cell antigens also exist. The radioligand-binding assay, which is convenient and sensitive for detecting GAD antibodies, will facilitate the screening of individuals with autoimmune islet cell disease." @default.
• W2135525868 created "2016-06-24" @default.
• W2135525868 creator A5009750098 @default.
• W2135525868 creator A5032103118 @default.
• W2135525868 creator A5036204726 @default.
• W2135525868 creator A5038720064 @default.
• W2135525868 creator A5051005847 @default.
• W2135525868 creator A5078801536 @default.
• W2135525868 date "1993-05-01" @default.
• W2135525868 modified "2023-09-26" @default.
• W2135525868 title "Demonstration of GAD-65 as the main immunogenic isoform of glutamate decarboxylase in type 1 diabetes and determination of autoantibodies using a radioligand produced by eukaryotic expression." @default.
• W2135525868 cites W1483887312 @default.
• W2135525868 cites W1546683674 @default.
• W2135525868 cites W1860160290 @default.
• W2135525868 cites W1977350311 @default.
• W2135525868 cites W1978885851 @default.
• W2135525868 cites W1982898602 @default.
• W2135525868 cites W1983864650 @default.
• W2135525868 cites W1987101799 @default.
• W2135525868 cites W1993325569 @default.
• W2135525868 cites W1994260912 @default.
• W2135525868 cites W1998484662 @default.
• W2135525868 cites W2019972832 @default.
• W2135525868 cites W2029438909 @default.
• W2135525868 cites W2029559333 @default.
• W2135525868 cites W2039743782 @default.
• W2135525868 cites W2048304876 @default.
• W2135525868 cites W2056799965 @default.
• W2135525868 cites W2068420002 @default.
• W2135525868 cites W2072547136 @default.
• W2135525868 cites W2072652145 @default.
• W2135525868 cites W2075290922 @default.
• W2135525868 cites W2075413718 @default.
• W2135525868 cites W2078165851 @default.
• W2135525868 cites W2079749800 @default.
• W2135525868 cites W2086870938 @default.
• W2135525868 cites W2088167128 @default.
• W2135525868 cites W2092251693 @default.
• W2135525868 cites W2105084892 @default.
• W2135525868 cites W2143868124 @default.
• W2135525868 cites W2146923243 @default.
• W2135525868 cites W2154100983 @default.
• W2135525868 cites W2168459132 @default.
• W2135525868 cites W2257784149 @default.
• W2135525868 doi "https://doi.org/10.1172/jci116431" @default.
• W2135525868 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/288207" @default.
• W2135525868 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8486775" @default.
• W2135525868 hasPublicationYear "1993" @default.
• W2135525868 type Work @default.
• W2135525868 sameAs 2135525868 @default.
• W2135525868 citedByCount "108" @default.
• W2135525868 countsByYear W21355258682012 @default.
• W2135525868 countsByYear W21355258682013 @default.
• W2135525868 countsByYear W21355258682014 @default.
• W2135525868 countsByYear W21355258682015 @default.
• W2135525868 countsByYear W21355258682016 @default.
• W2135525868 countsByYear W21355258682017 @default.
• W2135525868 countsByYear W21355258682019 @default.
• W2135525868 countsByYear W21355258682022 @default.
• W2135525868 countsByYear W21355258682023 @default.
• W2135525868 crossrefType "journal-article" @default.
• W2135525868 hasAuthorship W2135525868A5009750098 @default.
• W2135525868 hasAuthorship W2135525868A5032103118 @default.
• W2135525868 hasAuthorship W2135525868A5036204726 @default.
• W2135525868 hasAuthorship W2135525868A5038720064 @default.
• W2135525868 hasAuthorship W2135525868A5051005847 @default.
• W2135525868 hasAuthorship W2135525868A5078801536 @default.
• W2135525868 hasBestOaLocation W21355258681 @default.
• W2135525868 hasConcept C104317684 @default.
• W2135525868 hasConcept C134018914 @default.
• W2135525868 hasConcept C147483822 @default.
• W2135525868 hasConcept C153911025 @default.
• W2135525868 hasConcept C159654299 @default.
• W2135525868 hasConcept C163764329 @default.
• W2135525868 hasConcept C165220095 @default.
• W2135525868 hasConcept C181199279 @default.
• W2135525868 hasConcept C195616568 @default.
• W2135525868 hasConcept C203014093 @default.
• W2135525868 hasConcept C32611913 @default.
• W2135525868 hasConcept C40767141 @default.
• W2135525868 hasConcept C53345823 @default.
• W2135525868 hasConcept C55493867 @default.
• W2135525868 hasConcept C555293320 @default.
• W2135525868 hasConcept C86803240 @default.
• W2135525868 hasConcept C9497952 @default.
• W2135525868 hasConceptScore W2135525868C104317684 @default.
• W2135525868 hasConceptScore W2135525868C134018914 @default.
• W2135525868 hasConceptScore W2135525868C147483822 @default.
• W2135525868 hasConceptScore W2135525868C153911025 @default.
• W2135525868 hasConceptScore W2135525868C159654299 @default.
• W2135525868 hasConceptScore W2135525868C163764329 @default.
• W2135525868 hasConceptScore W2135525868C165220095 @default.
• W2135525868 hasConceptScore W2135525868C181199279 @default.
• W2135525868 hasConceptScore W2135525868C195616568 @default.
• W2135525868 hasConceptScore W2135525868C203014093 @default.
• W2135525868 hasConceptScore W2135525868C32611913 @default.
• W2135525868 hasConceptScore W2135525868C40767141 @default.
• W2135525868 hasConceptScore W2135525868C53345823 @default.

• next