FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2135546669> ?p ?o ?g. }

• W2135546669 endingPage "4070" @default.
• W2135546669 startingPage "4063" @default.
• W2135546669 abstract "FNR proteins are transcription regulators that sense changes in oxygen availability via assembly–disassembly of [4Fe–4S] clusters. The Escherichia coli FNR protein is present in bacteria grown under aerobic and anaerobic conditions. Under aerobic conditions, FNR is isolated as an inactive monomeric apoprotein, whereas under anaerobic conditions, FNR is present as an active dimeric holoprotein containing one [4Fe–4S] cluster per subunit. It has been suggested that the active and inactive forms of FNR are interconverted in vivo , or that iron–sulphur clusters are mostly incorporated into newly synthesized FNR. Here, experiments using a thermo-inducible fnr expression plasmid showed that a model FNR-dependent promoter is activated under anaerobic conditions by FNR that was synthesized under aerobic conditions. Immunoblots suggested that FNR was more prone to degradation under aerobic compared with anaerobic conditions, and that the ClpXP protease contributes to this degradation. Nevertheless, FNR was sufficiently long lived (half-life under aerobic conditions, ∼45 min) to allow cycling between active and inactive forms. Measuring the abundance of the FNR-activated dms transcript when chloramphenicol-treated cultures were switched between aerobic and anaerobic conditions showed that it increased when cultures were switched to anaerobic conditions, and decreased when aerobic conditions were restored. In contrast, measurement of the abundance of the FNR-repressed ndh transcript under the same conditions showed that it decreased upon switching to anaerobic conditions, and then increased when aerobic conditions were restored. The abundance of the FNR- and oxygen-independent tatE transcript was unaffected by changes in oxygen availability. Thus, the simplest explanation for the observations reported here is that the FNR protein can be switched between inactive and active forms in vivo in the absence of de novo protein synthesis." @default.
• W2135546669 created "2016-06-24" @default.
• W2135546669 creator A5042328891 @default.
• W2135546669 creator A5081759119 @default.
• W2135546669 date "2005-12-01" @default.
• W2135546669 modified "2023-09-23" @default.
• W2135546669 title "In vivo cycling of the Escherichia coli transcription factor FNR between active and inactive states" @default.
• W2135546669 cites W1589237644 @default.
• W2135546669 cites W1963025502 @default.
• W2135546669 cites W1966849007 @default.
• W2135546669 cites W1967842402 @default.
• W2135546669 cites W1974309223 @default.
• W2135546669 cites W1976033706 @default.
• W2135546669 cites W1977671794 @default.
• W2135546669 cites W1978134609 @default.
• W2135546669 cites W1980846858 @default.
• W2135546669 cites W1982309495 @default.
• W2135546669 cites W1983081312 @default.
• W2135546669 cites W1984882823 @default.
• W2135546669 cites W1995796760 @default.
• W2135546669 cites W2004046833 @default.
• W2135546669 cites W2006795645 @default.
• W2135546669 cites W2007493979 @default.
• W2135546669 cites W2010907389 @default.
• W2135546669 cites W2011788811 @default.
• W2135546669 cites W2022227481 @default.
• W2135546669 cites W2022863863 @default.
• W2135546669 cites W2033298896 @default.
• W2135546669 cites W2036985332 @default.
• W2135546669 cites W2038704829 @default.
• W2135546669 cites W2038950831 @default.
• W2135546669 cites W2042026721 @default.
• W2135546669 cites W2050599642 @default.
• W2135546669 cites W2069888431 @default.
• W2135546669 cites W2073364124 @default.
• W2135546669 cites W2082783701 @default.
• W2135546669 cites W2084834926 @default.
• W2135546669 cites W2090721016 @default.
• W2135546669 cites W2091936669 @default.
• W2135546669 cites W2109017709 @default.
• W2135546669 cites W2117650761 @default.
• W2135546669 cites W2124896722 @default.
• W2135546669 cites W2128095117 @default.
• W2135546669 cites W2129634655 @default.
• W2135546669 cites W2139405180 @default.
• W2135546669 cites W2140337827 @default.
• W2135546669 cites W2140449210 @default.
• W2135546669 cites W2143018675 @default.
• W2135546669 cites W2146171755 @default.
• W2135546669 cites W2149781820 @default.
• W2135546669 cites W2150618302 @default.
• W2135546669 cites W2150909918 @default.
• W2135546669 cites W2153930194 @default.
• W2135546669 cites W2155094355 @default.
• W2135546669 cites W2157488040 @default.
• W2135546669 cites W2166005059 @default.
• W2135546669 cites W2169583771 @default.
• W2135546669 cites W217428907 @default.
• W2135546669 cites W21947383 @default.
• W2135546669 cites W303860129 @default.
• W2135546669 cites W4244151339 @default.
• W2135546669 doi "https://doi.org/10.1099/mic.0.28253-0" @default.
• W2135546669 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16339951" @default.
• W2135546669 hasPublicationYear "2005" @default.
• W2135546669 type Work @default.
• W2135546669 sameAs 2135546669 @default.
• W2135546669 citedByCount "40" @default.
• W2135546669 countsByYear W21355466692012 @default.
• W2135546669 countsByYear W21355466692013 @default.
• W2135546669 countsByYear W21355466692014 @default.
• W2135546669 countsByYear W21355466692015 @default.
• W2135546669 countsByYear W21355466692016 @default.
• W2135546669 countsByYear W21355466692017 @default.
• W2135546669 countsByYear W21355466692018 @default.
• W2135546669 countsByYear W21355466692019 @default.
• W2135546669 countsByYear W21355466692020 @default.
• W2135546669 countsByYear W21355466692021 @default.
• W2135546669 crossrefType "journal-article" @default.
• W2135546669 hasAuthorship W2135546669A5042328891 @default.
• W2135546669 hasAuthorship W2135546669A5081759119 @default.
• W2135546669 hasBestOaLocation W21355466691 @default.
• W2135546669 hasConcept C104317684 @default.
• W2135546669 hasConcept C138885662 @default.
• W2135546669 hasConcept C178790620 @default.
• W2135546669 hasConcept C179926584 @default.
• W2135546669 hasConcept C185592680 @default.
• W2135546669 hasConcept C41895202 @default.
• W2135546669 hasConcept C42407357 @default.
• W2135546669 hasConcept C48843007 @default.
• W2135546669 hasConcept C5140985 @default.
• W2135546669 hasConcept C523546767 @default.
• W2135546669 hasConcept C540031477 @default.
• W2135546669 hasConcept C54355233 @default.
• W2135546669 hasConcept C547475151 @default.
• W2135546669 hasConcept C55493867 @default.
• W2135546669 hasConcept C86803240 @default.
• W2135546669 hasConcept C89423630 @default.
• W2135546669 hasConceptScore W2135546669C104317684 @default.

• next