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• W2135656393 startingPage "5129" @default.
• W2135656393 abstract "ABSTRACT In Methanothermobacter thermautotrophicus , oxaloacetate synthesis is a major and essential CO 2 -fixation reaction. This methanogenic archaeon possesses two oxaloacetate-synthesizing enzymes, pyruvate carboxylase and phosphoenolpyruvate carboxylase. The phosphoenolpyruvate carboxylase from this organism was purified to homogeneity. The subunit size of this homotetrameric protein was 55 kDa, which is about half that of all known bacterial and eukaryotic phosphoenolpyruvate carboxylases (PPCs). The NH 2 -terminal sequence identified this enzyme as the product of MTH943, an open reading frame with no assigned function in the genome sequence. A BLAST search did not show an obvious sequence similarity between MTH943 and known PPCs, which are generally well conserved. This is the first report of a new type of phosphoenolpyruvate carboxylase that we call PpcA (“A” for “archaeal”). Homologs to PpcA were present in most archaeal genomic sequences, but only in three bacterial ( Clostridium perfringens , Oenococcus oeni , and Leuconostoc mesenteroides ) and no eukaryotic genomes. PpcA was the only recognizable oxaloacetate-producing enzyme in Methanopyrus kandleri , a hydrothermal vent organism. Each PpcA-containing organism lacked a PPC homolog. The activity of M. thermautotrophicus PpcA was not influenced by acetyl coenzyme A and was about 50 times less sensitive to aspartate than the Escherichia coli PPC. The catalytic core (including His 138 , Arg 587 , and Gly 883 ) of the E. coli PPC was partly conserved in PpcA, but three of four aspartate-binding residues (Lys 773 , Arg 832 , and Asn 881 ) were not. PPCs probably evolved from PpcA through a process that added allosteric sites to the enzyme. The reverse is also equally possible." @default.
• W2135656393 created "2016-06-24" @default.
• W2135656393 creator A5020534066 @default.
• W2135656393 creator A5027814777 @default.
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• W2135656393 date "2004-08-01" @default.
• W2135656393 modified "2023-10-01" @default.
• W2135656393 title "The Phosphoenolpyruvate Carboxylase from <i>Methanothermobacter thermautotrophicus</i> Has a Novel Structure" @default.
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• W2135656393 doi "https://doi.org/10.1128/jb.186.15.5129-5137.2004" @default.
• W2135656393 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/451628" @default.
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