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- W2135767067 abstract "Summary. Diapausing mouse blastocysts were cultured in vitro with inhibitors of polyamine synthesis and/or the polyamines, putrescine, spermidine and/or spermine. Attachment of blastocysts to the culture dish, the onset of trophoblastic outgrowth and the extent of outgrowth were retarded by methylornithine (10 mm) or methylglyoxal bis-(guanylhydrazone) (50 or 200 μm) and apparently blocked by a combination of these inhibitors. The embryos appeared to make no progress towards activation in the presence of both inhibitors but became active and grew normally when transferred to inhibitor-free medium. The inhibition of growth was also reversed when polyamines (10−5 m) were supplied in the medium along with the inhibitors (P < 0·01). These results suggest that polyamine synthesis is required for activation, growth and development of diapausing blastocysts in vitro." @default.
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- W2135767067 title "Effect of inhibitors of polyamine synthesis on activation of diapausing mouse blastocysts in vitro" @default.
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- W2135767067 doi "https://doi.org/10.1530/jrf.0.0680437" @default.
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