FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2135841502> ?p ?o ?g. }

• W2135841502 endingPage "1701" @default.
• W2135841502 startingPage "1687" @default.
• W2135841502 abstract "Guanosines are important for biological activities through their specific functional groups that are recognized for RNA or protein interactions. One example is recognition of N 1 of G37 in tRNA by S -adenosyl-methionine (AdoMet)–dependent tRNA methyltransferases to synthesize m 1 G37-tRNA, which is essential for translational fidelity in all biological domains. Synthesis of m 1 G37-tRNA is catalyzed by TrmD in bacteria and by Trm5 in eukarya and archaea, using unrelated and dissimilar structural folds. This raises the question of how dissimilar proteins recognize the same guanosine. Here we probe the mechanism of discrimination among functional groups of guanosine by TrmD and Trm5. Guanosine analogs were systematically introduced into tRNA through a combination of chemical and enzymatic synthesis. Single turnover kinetic assays and thermodynamic analysis of the effect of each analog on m 1 G37-tRNA synthesis reveal that TrmD and Trm5 discriminate functional groups differently. While both recognize N 1 and O 6 of G37, TrmD places a much stronger emphasis on these functional groups than Trm5. While the exocyclic 2-amino group of G37 is important for TrmD, it is dispensable for Trm5. In addition, while an adjacent G36 is obligatory for TrmD, it is nonessential for Trm5. These results depict a more rigid requirement of guanosine functional groups for TrmD than for Trm5. However, the sensitivity of both enzymes to analog substitutions, together with an experimental revelation of their low cellular concentrations relative to tRNA substrates, suggests a model in which these enzymes rapidly screen tRNA by direct recognition of G37 in order to monitor the global state of m 1 G37-tRNA." @default.
• W2135841502 created "2016-06-24" @default.
• W2135841502 creator A5012805315 @default.
• W2135841502 creator A5015680964 @default.
• W2135841502 creator A5019769228 @default.
• W2135841502 creator A5024418192 @default.
• W2135841502 creator A5032485134 @default.
• W2135841502 creator A5083492111 @default.
• W2135841502 creator A5085148313 @default.
• W2135841502 creator A5086859418 @default.
• W2135841502 creator A5089964310 @default.
• W2135841502 date "2012-07-30" @default.
• W2135841502 modified "2023-10-13" @default.
• W2135841502 title "Recognition of guanosine by dissimilar tRNA methyltransferases" @default.
• W2135841502 cites W1532644721 @default.
• W2135841502 cites W1867958153 @default.
• W2135841502 cites W1968143368 @default.
• W2135841502 cites W1969211306 @default.
• W2135841502 cites W1972433022 @default.
• W2135841502 cites W1977738109 @default.
• W2135841502 cites W1980412333 @default.
• W2135841502 cites W1981791357 @default.
• W2135841502 cites W1992426305 @default.
• W2135841502 cites W1996135412 @default.
• W2135841502 cites W2002036062 @default.
• W2135841502 cites W2006074942 @default.
• W2135841502 cites W2013268349 @default.
• W2135841502 cites W2013728170 @default.
• W2135841502 cites W2019729932 @default.
• W2135841502 cites W2022082484 @default.
• W2135841502 cites W2026004144 @default.
• W2135841502 cites W2028971512 @default.
• W2135841502 cites W2029047779 @default.
• W2135841502 cites W2029318943 @default.
• W2135841502 cites W2029551024 @default.
• W2135841502 cites W2031045177 @default.
• W2135841502 cites W2033184129 @default.
• W2135841502 cites W2035926239 @default.
• W2135841502 cites W2037825700 @default.
• W2135841502 cites W2039067448 @default.
• W2135841502 cites W2041730140 @default.
• W2135841502 cites W2046189659 @default.
• W2135841502 cites W2048858950 @default.
• W2135841502 cites W2053238521 @default.
• W2135841502 cites W2054511300 @default.
• W2135841502 cites W2057781025 @default.
• W2135841502 cites W2061357866 @default.
• W2135841502 cites W2065725400 @default.
• W2135841502 cites W2080611048 @default.
• W2135841502 cites W2084788365 @default.
• W2135841502 cites W2093471887 @default.
• W2135841502 cites W2095120004 @default.
• W2135841502 cites W2098969130 @default.
• W2135841502 cites W2100071542 @default.
• W2135841502 cites W2111967267 @default.
• W2135841502 cites W2114857744 @default.
• W2135841502 cites W2117492058 @default.
• W2135841502 cites W2127408623 @default.
• W2135841502 cites W2133666260 @default.
• W2135841502 cites W2134326554 @default.
• W2135841502 cites W2139307369 @default.
• W2135841502 cites W2148604640 @default.
• W2135841502 cites W2150409129 @default.
• W2135841502 cites W2154208264 @default.
• W2135841502 cites W2155187886 @default.
• W2135841502 cites W2162759883 @default.
• W2135841502 cites W2202722181 @default.
• W2135841502 doi "https://doi.org/10.1261/rna.032029.111" @default.
• W2135841502 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3425783" @default.
• W2135841502 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22847817" @default.
• W2135841502 hasPublicationYear "2012" @default.
• W2135841502 type Work @default.
• W2135841502 sameAs 2135841502 @default.
• W2135841502 citedByCount "30" @default.
• W2135841502 countsByYear W21358415022013 @default.
• W2135841502 countsByYear W21358415022014 @default.
• W2135841502 countsByYear W21358415022015 @default.
• W2135841502 countsByYear W21358415022016 @default.
• W2135841502 countsByYear W21358415022017 @default.
• W2135841502 countsByYear W21358415022018 @default.
• W2135841502 countsByYear W21358415022019 @default.
• W2135841502 countsByYear W21358415022020 @default.
• W2135841502 countsByYear W21358415022021 @default.
• W2135841502 countsByYear W21358415022022 @default.
• W2135841502 countsByYear W21358415022023 @default.
• W2135841502 crossrefType "journal-article" @default.
• W2135841502 hasAuthorship W2135841502A5012805315 @default.
• W2135841502 hasAuthorship W2135841502A5015680964 @default.
• W2135841502 hasAuthorship W2135841502A5019769228 @default.
• W2135841502 hasAuthorship W2135841502A5024418192 @default.
• W2135841502 hasAuthorship W2135841502A5032485134 @default.
• W2135841502 hasAuthorship W2135841502A5083492111 @default.
• W2135841502 hasAuthorship W2135841502A5085148313 @default.
• W2135841502 hasAuthorship W2135841502A5086859418 @default.
• W2135841502 hasAuthorship W2135841502A5089964310 @default.
• W2135841502 hasBestOaLocation W21358415021 @default.
• W2135841502 hasConcept C104317684 @default.
• W2135841502 hasConcept C153957851 @default.

• next