Matches in SemOpenAlex for { <https://semopenalex.org/work/W2135890751> ?p ?o ?g. }
- W2135890751 endingPage "2059" @default.
- W2135890751 startingPage "2049" @default.
- W2135890751 abstract "Activation of Rac1 GTPase signaling is stimulated by phosphorylation and release of RhoGDI by the effector p21-activated kinase 1 (PAK1), but it is unclear what initiates this potential feed-forward mechanism for regulation of Rac activity. Phosphatidic acid (PA), which is produced from the lipid second messenger diacylglycerol (DAG) by the action of DAG kinases (DGKs), is known to activate PAK1. Here, we investigated whether PA produced by DGKζ initiates RhoGDI release and Rac1 activation. In DGKζ-deficient fibroblasts PAK1 phosphorylation and Rac1–RhoGDI dissociation were attenuated, leading to reduced Rac1 activation after platelet-derived growth factor stimulation. The cells were defective in Rac1-regulated behaviors, including lamellipodia formation, membrane ruffling, migration, and spreading. Wild-type DGKζ, but not a kinase-dead mutant, or addition of exogenous PA rescued Rac activation. DGKζ stably associated with PAK1 and RhoGDI, suggesting these proteins form a complex that functions as a Rac1-selective RhoGDI dissociation factor. These results define a pathway that links diacylglycerol, DGKζ, and PA to the activation of Rac1: the PA generated by DGKζ activates PAK1, which dissociates RhoGDI from Rac1 leading to changes in actin dynamics that facilitate the changes necessary for cell motility." @default.
- W2135890751 created "2016-06-24" @default.
- W2135890751 creator A5010450016 @default.
- W2135890751 creator A5047115191 @default.
- W2135890751 creator A5053101911 @default.
- W2135890751 creator A5071316993 @default.
- W2135890751 creator A5076944041 @default.
- W2135890751 creator A5088723847 @default.
- W2135890751 creator A5089917103 @default.
- W2135890751 date "2009-04-01" @default.
- W2135890751 modified "2023-10-18" @default.
- W2135890751 title "Diacylglycerol Kinase ζ Regulates Actin Cytoskeleton Reorganization through Dissociation of Rac1 from RhoGDI" @default.
- W2135890751 cites W1233997596 @default.
- W2135890751 cites W1490646520 @default.
- W2135890751 cites W1525274814 @default.
- W2135890751 cites W1575506386 @default.
- W2135890751 cites W1591948006 @default.
- W2135890751 cites W1593328663 @default.
- W2135890751 cites W1826668344 @default.
- W2135890751 cites W1963756782 @default.
- W2135890751 cites W1968551382 @default.
- W2135890751 cites W1968826282 @default.
- W2135890751 cites W1975981720 @default.
- W2135890751 cites W1978218402 @default.
- W2135890751 cites W1984967137 @default.
- W2135890751 cites W1986349419 @default.
- W2135890751 cites W1989987282 @default.
- W2135890751 cites W1994241233 @default.
- W2135890751 cites W2002440150 @default.
- W2135890751 cites W2004078164 @default.
- W2135890751 cites W2004375684 @default.
- W2135890751 cites W2008710902 @default.
- W2135890751 cites W2009662773 @default.
- W2135890751 cites W2013149045 @default.
- W2135890751 cites W2019012834 @default.
- W2135890751 cites W2019064734 @default.
- W2135890751 cites W2022807358 @default.
- W2135890751 cites W2024309614 @default.
- W2135890751 cites W2025591259 @default.
- W2135890751 cites W2027810531 @default.
- W2135890751 cites W2028220020 @default.
- W2135890751 cites W2028940392 @default.
- W2135890751 cites W2031874417 @default.
- W2135890751 cites W2033366715 @default.
- W2135890751 cites W2039205173 @default.
- W2135890751 cites W2046966521 @default.
- W2135890751 cites W2049967046 @default.
- W2135890751 cites W2055289589 @default.
- W2135890751 cites W2059279105 @default.
- W2135890751 cites W2061171666 @default.
- W2135890751 cites W2062556999 @default.
- W2135890751 cites W2063538689 @default.
- W2135890751 cites W2064338173 @default.
- W2135890751 cites W2068772079 @default.
- W2135890751 cites W2074867744 @default.
- W2135890751 cites W2077080652 @default.
- W2135890751 cites W2079274639 @default.
- W2135890751 cites W2081898060 @default.
- W2135890751 cites W2086405676 @default.
- W2135890751 cites W2089234963 @default.
- W2135890751 cites W2093226717 @default.
- W2135890751 cites W2102312968 @default.
- W2135890751 cites W2116085905 @default.
- W2135890751 cites W2118060829 @default.
- W2135890751 cites W2118461920 @default.
- W2135890751 cites W2121810961 @default.
- W2135890751 cites W2123659424 @default.
- W2135890751 cites W2127441616 @default.
- W2135890751 cites W2129277563 @default.
- W2135890751 cites W2130172628 @default.
- W2135890751 cites W2142055861 @default.
- W2135890751 cites W2143045155 @default.
- W2135890751 cites W2144026996 @default.
- W2135890751 cites W2149290707 @default.
- W2135890751 cites W2149352954 @default.
- W2135890751 cites W2155087173 @default.
- W2135890751 cites W2157627838 @default.
- W2135890751 cites W2160114186 @default.
- W2135890751 cites W2165525793 @default.
- W2135890751 cites W2166023528 @default.
- W2135890751 cites W2167158605 @default.
- W2135890751 cites W4246590628 @default.
- W2135890751 doi "https://doi.org/10.1091/mbc.e07-12-1248" @default.
- W2135890751 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2663923" @default.
- W2135890751 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19211846" @default.
- W2135890751 hasPublicationYear "2009" @default.
- W2135890751 type Work @default.
- W2135890751 sameAs 2135890751 @default.
- W2135890751 citedByCount "72" @default.
- W2135890751 countsByYear W21358907512012 @default.
- W2135890751 countsByYear W21358907512013 @default.
- W2135890751 countsByYear W21358907512014 @default.
- W2135890751 countsByYear W21358907512015 @default.
- W2135890751 countsByYear W21358907512016 @default.
- W2135890751 countsByYear W21358907512017 @default.
- W2135890751 countsByYear W21358907512018 @default.
- W2135890751 countsByYear W21358907512019 @default.
- W2135890751 countsByYear W21358907512020 @default.