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• W2135893169 endingPage "e77767" @default.
• W2135893169 startingPage "e77767" @default.
• W2135893169 abstract "Type III secretion is a tightly controlled virulence mechanism utilized by many gram negative bacteria to colonize their eukaryotic hosts. To infect their host, human pathogenic Yersinia spp. translocate protein toxins into the host cell cytosol through a preassembled Ysc-Yop type III secretion device. Several of the Ysc-Yop components are known for their roles in controlling substrate secretion and translocation. Particularly important in this role is the YopN and TyeA heterodimer. In this study, we confirm that Y. pseudotuberculosis naturally produce a 42 kDa YopN-TyeA hybrid protein as a result of a +1 frame shift near the 3 prime of yopN mRNA, as has been previously reported for the closely related Y. pestis. To assess the biological role of this YopN-TyeA hybrid in T3SS by Y. pseudotuberculosis, we used in cis site-directed mutagenesis to engineer bacteria to either produce predominately the YopN-TyeA hybrid by introducing +1 frame shifts to yopN after codon 278 or 287, or to produce only singular YopN and TyeA polypeptides by introducing yopN sequence from Y. enterocolitica, which is known not to produce the hybrid. Significantly, the engineered 42 kDa YopN-TyeA fusions were abundantly produced, stable, and were efficiently secreted by bacteria in vitro. Moreover, these bacteria could all maintain functionally competent needle structures and controlled Yops secretion in vitro. In the presence of host cells however, bacteria producing the most genetically altered hybrids (+1 frameshift after 278 codon) had diminished control of polarized Yop translocation. This corresponded to significant attenuation in competitive survival assays in orally infected mice, although not at all to the same extent as Yersinia lacking both YopN and TyeA proteins. Based on these studies with engineered polypeptides, most likely a naturally occurring YopN-TyeA hybrid protein has the potential to influence T3S control and activity when produced during Yersinia-host cell contact." @default.
• W2135893169 created "2016-06-24" @default.
• W2135893169 creator A5001515232 @default.
• W2135893169 creator A5002461356 @default.
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• W2135893169 creator A5040654589 @default.
• W2135893169 creator A5055807985 @default.
• W2135893169 creator A5064744168 @default.
• W2135893169 date "2013-10-03" @default.
• W2135893169 modified "2023-10-16" @default.
• W2135893169 title "Genetically Engineered Frameshifted YopN-TyeA Chimeras Influence Type III Secretion System Function in Yersinia pseudotuberculosis" @default.
• W2135893169 cites W1495119128 @default.
• W2135893169 cites W1541186321 @default.
• W2135893169 cites W1559661404 @default.
• W2135893169 cites W1567074356 @default.
• W2135893169 cites W158273916 @default.
• W2135893169 cites W1605977861 @default.
• W2135893169 cites W1785543522 @default.
• W2135893169 cites W1866548929 @default.
• W2135893169 cites W1961357949 @default.
• W2135893169 cites W1963510575 @default.
• W2135893169 cites W1966881422 @default.
• W2135893169 cites W1979137588 @default.
• W2135893169 cites W1983176420 @default.
• W2135893169 cites W1986881659 @default.
• W2135893169 cites W1988087263 @default.
• W2135893169 cites W1989798239 @default.
• W2135893169 cites W1999411039 @default.
• W2135893169 cites W2006635484 @default.
• W2135893169 cites W2014043692 @default.
• W2135893169 cites W2014216245 @default.
• W2135893169 cites W2015759788 @default.
• W2135893169 cites W2016814816 @default.
• W2135893169 cites W2026418105 @default.
• W2135893169 cites W2027181869 @default.
• W2135893169 cites W2027229485 @default.
• W2135893169 cites W2038354483 @default.
• W2135893169 cites W2038521075 @default.
• W2135893169 cites W2039756922 @default.
• W2135893169 cites W2042447268 @default.
• W2135893169 cites W2043713273 @default.
• W2135893169 cites W2044085349 @default.
• W2135893169 cites W2047091751 @default.
• W2135893169 cites W2050550262 @default.
• W2135893169 cites W2055586767 @default.
• W2135893169 cites W2056525859 @default.
• W2135893169 cites W2067731330 @default.
• W2135893169 cites W2075839343 @default.
• W2135893169 cites W2080098971 @default.
• W2135893169 cites W2081966923 @default.
• W2135893169 cites W2084898137 @default.
• W2135893169 cites W2091528429 @default.
• W2135893169 cites W2091921368 @default.
• W2135893169 cites W2092035637 @default.
• W2135893169 cites W2092967278 @default.
• W2135893169 cites W2099399224 @default.
• W2135893169 cites W2104790843 @default.
• W2135893169 cites W2106240534 @default.
• W2135893169 cites W2108099417 @default.
• W2135893169 cites W2108649941 @default.
• W2135893169 cites W2109810929 @default.
• W2135893169 cites W2114356152 @default.
• W2135893169 cites W2115047987 @default.
• W2135893169 cites W2117072224 @default.
• W2135893169 cites W2117772438 @default.
• W2135893169 cites W2119570097 @default.
• W2135893169 cites W2124453620 @default.
• W2135893169 cites W2125701320 @default.
• W2135893169 cites W2127058760 @default.
• W2135893169 cites W2130399739 @default.
• W2135893169 cites W2131795398 @default.
• W2135893169 cites W2132007061 @default.
• W2135893169 cites W2134410571 @default.
• W2135893169 cites W2140795925 @default.
• W2135893169 cites W2141037335 @default.
• W2135893169 cites W2141219835 @default.
• W2135893169 cites W2144579151 @default.
• W2135893169 cites W2144935043 @default.
• W2135893169 cites W2148742847 @default.
• W2135893169 cites W2151394231 @default.
• W2135893169 cites W2152245097 @default.
• W2135893169 cites W2152482656 @default.
• W2135893169 cites W2153463868 @default.
• W2135893169 cites W2153531943 @default.
• W2135893169 cites W2155184228 @default.
• W2135893169 cites W2156328657 @default.
• W2135893169 cites W2156405992 @default.
• W2135893169 cites W2157462470 @default.
• W2135893169 cites W2157735947 @default.
• W2135893169 cites W2158369156 @default.
• W2135893169 cites W2160090062 @default.
• W2135893169 cites W2162103629 @default.
• W2135893169 cites W2162733568 @default.
• W2135893169 cites W2163023456 @default.
• W2135893169 cites W2165167163 @default.
• W2135893169 cites W2172070570 @default.
• W2135893169 cites W2185510425 @default.
• W2135893169 cites W2316213795 @default.

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