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• W2135906095 abstract "Background and purpose: Toxoplasmosis is a common parasitic disease throughout the world and one- third of the population has antibodies to Toxoplasma gondii. This disease causes serious medical problems in f etuses and immunocompromised individuals. As gene encoding protein GRA14 can be considered as a suitable targ et for DNA vaccine and designing diagnostic kits; the aim of this study was to do cloning and sequencing the gene encoding GRA14 protein of Toxoplasma gondii RH strain. Materials and methods: DNA extraction was performed on harvested tachyzoites from mouse peritoneal fluid, then PCR carried out and amplification produ cts were analyzed by gel electrophoresis. GRA14 gene was cloned in pTG19-T as a cloning vector, then recombinant pl asmid confirmed by the colony-PCR and restriction enzyme digestion using HindIII and EcoRI, followed by sequencing. Results: Evaluation of PCR products by agarose gel electrop horesis and analysis of nucleotide sequencing of 1227 bp gene encoding the protein GRA14, revealed the complete homology with the recorded sequences in the gene bank. Furthermore, cloning of GRA14 gene in pTG19-T vector was confirmed with colony PCR and restriction enzyme digestion. Conclusion: The results showed that the GRA14 gene was successfully cloned into the pTG19-T vector and this plasmid can be used to design DNA vaccines and diagnostic kits in further studies." @default.
• W2135906095 created "2016-06-24" @default.
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• W2135906095 date "2014-05-15" @default.
• W2135906095 modified "2023-09-27" @default.
• W2135906095 title "Cloning and sequencing the plasmid encoding dense granule antigen 14 (GRA14) of Toxoplasma gondii RH strain" @default.
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