Matches in SemOpenAlex for { <https://semopenalex.org/work/W2135984124> ?p ?o ?g. }
- W2135984124 endingPage "3500" @default.
- W2135984124 startingPage "3500" @default.
- W2135984124 abstract "We have produced ultra-small sized (20–50 nm diameter) calcium phosphate (CaP) nanoparticles encapsulating a reporter gene, pCambia 1301. The material in the nanoparticles is of crystalline nature having a hydroxyapatite structure as revealed from the XRD pattern. The maximum loading of pCambia 1301 in the nanoparticles and the pH-dependent dissolution of CaP nanoparticles were studied using gel electrophoresis. DNA is highly protected in the cell from cellular nucleases when it is encapsulated into the CaP nanoparticles. The transformation efficiency was found to be about 80.7% compared to 54.4% by Agrobacterium tumefaciens and only 8% using naked DNA. Our results indicate that CaP nanoparticles could be used as a better transforming vector in plants as compared to the Agrobacterium tumefaciens mediated genetic transformation technique. In our experiment we presume that the plasmid DNA released from CaP nanoparticles in the cell has, perhaps, been able to enter into the nucleus. Transgenic GUS (β-glucuronidase) integrates into the genomic DNA by non-homologous recombination as in the case of Agrobacterium tumefaciens infection. Because of high transformation frequency, the method seems to be an attractive option for delivering a transgene into plant cells and protoplast." @default.
- W2135984124 created "2016-06-24" @default.
- W2135984124 creator A5042928471 @default.
- W2135984124 creator A5053623556 @default.
- W2135984124 creator A5056714631 @default.
- W2135984124 creator A5060411079 @default.
- W2135984124 creator A5080209433 @default.
- W2135984124 creator A5091710642 @default.
- W2135984124 date "2012-01-01" @default.
- W2135984124 modified "2023-10-12" @default.
- W2135984124 title "Calcium phosphate nanoparticle mediated genetic transformation in plants" @default.
- W2135984124 cites W1539503448 @default.
- W2135984124 cites W1577071491 @default.
- W2135984124 cites W1588088313 @default.
- W2135984124 cites W1941973560 @default.
- W2135984124 cites W1976876678 @default.
- W2135984124 cites W2001141372 @default.
- W2135984124 cites W2003493943 @default.
- W2135984124 cites W2009886197 @default.
- W2135984124 cites W2019410656 @default.
- W2135984124 cites W2023689726 @default.
- W2135984124 cites W2024556021 @default.
- W2135984124 cites W2026082338 @default.
- W2135984124 cites W2031017018 @default.
- W2135984124 cites W2036528771 @default.
- W2135984124 cites W2039483927 @default.
- W2135984124 cites W2052813349 @default.
- W2135984124 cites W2060037459 @default.
- W2135984124 cites W2066344548 @default.
- W2135984124 cites W2073345066 @default.
- W2135984124 cites W2080218443 @default.
- W2135984124 cites W2085255220 @default.
- W2135984124 cites W2091664457 @default.
- W2135984124 cites W2094504947 @default.
- W2135984124 cites W2110303380 @default.
- W2135984124 cites W2117219833 @default.
- W2135984124 cites W2124691279 @default.
- W2135984124 cites W2136060053 @default.
- W2135984124 cites W2140177630 @default.
- W2135984124 cites W2144553472 @default.
- W2135984124 cites W2146810518 @default.
- W2135984124 cites W2151765209 @default.
- W2135984124 cites W2160945564 @default.
- W2135984124 cites W2163486371 @default.
- W2135984124 cites W71344925 @default.
- W2135984124 doi "https://doi.org/10.1039/c2jm11739h" @default.
- W2135984124 hasPublicationYear "2012" @default.
- W2135984124 type Work @default.
- W2135984124 sameAs 2135984124 @default.
- W2135984124 citedByCount "63" @default.
- W2135984124 countsByYear W21359841242013 @default.
- W2135984124 countsByYear W21359841242014 @default.
- W2135984124 countsByYear W21359841242016 @default.
- W2135984124 countsByYear W21359841242017 @default.
- W2135984124 countsByYear W21359841242018 @default.
- W2135984124 countsByYear W21359841242019 @default.
- W2135984124 countsByYear W21359841242020 @default.
- W2135984124 countsByYear W21359841242021 @default.
- W2135984124 countsByYear W21359841242022 @default.
- W2135984124 countsByYear W21359841242023 @default.
- W2135984124 crossrefType "journal-article" @default.
- W2135984124 hasAuthorship W2135984124A5042928471 @default.
- W2135984124 hasAuthorship W2135984124A5053623556 @default.
- W2135984124 hasAuthorship W2135984124A5056714631 @default.
- W2135984124 hasAuthorship W2135984124A5060411079 @default.
- W2135984124 hasAuthorship W2135984124A5080209433 @default.
- W2135984124 hasAuthorship W2135984124A5091710642 @default.
- W2135984124 hasConcept C102230213 @default.
- W2135984124 hasConcept C104317684 @default.
- W2135984124 hasConcept C122172344 @default.
- W2135984124 hasConcept C12554922 @default.
- W2135984124 hasConcept C142613039 @default.
- W2135984124 hasConcept C147789679 @default.
- W2135984124 hasConcept C150194340 @default.
- W2135984124 hasConcept C153911025 @default.
- W2135984124 hasConcept C155672457 @default.
- W2135984124 hasConcept C161733203 @default.
- W2135984124 hasConcept C171250308 @default.
- W2135984124 hasConcept C178050479 @default.
- W2135984124 hasConcept C178790620 @default.
- W2135984124 hasConcept C182420341 @default.
- W2135984124 hasConcept C185592680 @default.
- W2135984124 hasConcept C192562407 @default.
- W2135984124 hasConcept C195856716 @default.
- W2135984124 hasConcept C204241405 @default.
- W2135984124 hasConcept C22744801 @default.
- W2135984124 hasConcept C2776162087 @default.
- W2135984124 hasConcept C2779656422 @default.
- W2135984124 hasConcept C519063684 @default.
- W2135984124 hasConcept C552990157 @default.
- W2135984124 hasConcept C55493867 @default.
- W2135984124 hasConcept C86803240 @default.
- W2135984124 hasConcept C88380143 @default.
- W2135984124 hasConceptScore W2135984124C102230213 @default.
- W2135984124 hasConceptScore W2135984124C104317684 @default.
- W2135984124 hasConceptScore W2135984124C122172344 @default.
- W2135984124 hasConceptScore W2135984124C12554922 @default.
- W2135984124 hasConceptScore W2135984124C142613039 @default.