Matches in SemOpenAlex for { <https://semopenalex.org/work/W2136094884> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2136094884 endingPage "65" @default.
- W2136094884 startingPage "59" @default.
- W2136094884 abstract "Mechanical loading is a well-known regulator of cartilage metabolism. This suggests that a loading-induced physical signal regulates chondrocyte behavior. Previous studies have focused on the effects of steady fluid flow on chondrocytes. In contrast to steady flow, loading induced fluid flow occurs in an oscillatory pattern and includes a reversal of flow direction with each loading event. In this study we examined the hypothesis that oscillating fluid flow increases cytosolic Ca2+ concentration ([Ca2+]i) in bovine articular chondrocytes (BAC) in a frequency-dependent manner and that the presence of serum affects this response. The aims of our study were to examine (1) whether BAC respond to physiologic oscillating fluid flow in vitro and compare these results to steady fluid flow, (2) the effect of fetal bovine serum on fluid flow responsiveness of BAC and (3) whether the response of BAC to fluid flow is flow rate and/or frequency dependent. [Ca2+]i was quantified using the fluorescent dye fura-2. BAC were exposed to steady, 0.5, 1, or 5 Hz sinusoidal oscillating fluid flow at five different flow rates in a parallel plate flow chamber. Our findings demonstrate that BAC respond to oscillating fluid flow with an increase in [Ca2+]i (p > 0.05), and furthermore, chondrocyte responsiveness to fluid flow increases with peak flow rate (p < 0.0001) and decreases with increasing frequencies (p < 0.0001). Finally, the presence of serum in the media potentiated the responsiveness of BAC to fluid flow (p < 0.0001). Our results suggest an important role for mechanical load-induced oscillating fluid flow in chondrocyte mechanotransduction." @default.
- W2136094884 created "2016-06-24" @default.
- W2136094884 creator A5007737311 @default.
- W2136094884 creator A5069686409 @default.
- W2136094884 creator A5073012036 @default.
- W2136094884 creator A5086137850 @default.
- W2136094884 date "2001-01-01" @default.
- W2136094884 modified "2023-10-16" @default.
- W2136094884 title "Oscillating fluid flow regulates cytosolic calcium concentration in bovine articular chondrocytes" @default.
- W2136094884 cites W105470317 @default.
- W2136094884 cites W1537328568 @default.
- W2136094884 cites W1936770188 @default.
- W2136094884 cites W1994311932 @default.
- W2136094884 cites W1995039844 @default.
- W2136094884 cites W1995120265 @default.
- W2136094884 cites W2009350275 @default.
- W2136094884 cites W2014471803 @default.
- W2136094884 cites W2051761181 @default.
- W2136094884 cites W2058353653 @default.
- W2136094884 cites W2060177572 @default.
- W2136094884 cites W2077741258 @default.
- W2136094884 cites W2081257648 @default.
- W2136094884 cites W2083682299 @default.
- W2136094884 cites W2113405430 @default.
- W2136094884 cites W2135197803 @default.
- W2136094884 cites W2145937652 @default.
- W2136094884 cites W2147237892 @default.
- W2136094884 cites W2155937147 @default.
- W2136094884 cites W2169731710 @default.
- W2136094884 cites W2403153441 @default.
- W2136094884 cites W2461168023 @default.
- W2136094884 cites W3149337173 @default.
- W2136094884 cites W4235622590 @default.
- W2136094884 doi "https://doi.org/10.1016/s0021-9290(00)00158-5" @default.
- W2136094884 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11425081" @default.
- W2136094884 hasPublicationYear "2001" @default.
- W2136094884 type Work @default.
- W2136094884 sameAs 2136094884 @default.
- W2136094884 citedByCount "52" @default.
- W2136094884 countsByYear W21360948842012 @default.
- W2136094884 countsByYear W21360948842013 @default.
- W2136094884 countsByYear W21360948842014 @default.
- W2136094884 countsByYear W21360948842015 @default.
- W2136094884 countsByYear W21360948842017 @default.
- W2136094884 countsByYear W21360948842018 @default.
- W2136094884 countsByYear W21360948842019 @default.
- W2136094884 countsByYear W21360948842021 @default.
- W2136094884 countsByYear W21360948842022 @default.
- W2136094884 crossrefType "journal-article" @default.
- W2136094884 hasAuthorship W2136094884A5007737311 @default.
- W2136094884 hasAuthorship W2136094884A5069686409 @default.
- W2136094884 hasAuthorship W2136094884A5073012036 @default.
- W2136094884 hasAuthorship W2136094884A5086137850 @default.
- W2136094884 hasConcept C121332964 @default.
- W2136094884 hasConcept C12554922 @default.
- W2136094884 hasConcept C185592680 @default.
- W2136094884 hasConcept C202751555 @default.
- W2136094884 hasConcept C2781403057 @default.
- W2136094884 hasConcept C38349280 @default.
- W2136094884 hasConcept C55493867 @default.
- W2136094884 hasConcept C57879066 @default.
- W2136094884 hasConcept C86803240 @default.
- W2136094884 hasConcept C90278072 @default.
- W2136094884 hasConceptScore W2136094884C121332964 @default.
- W2136094884 hasConceptScore W2136094884C12554922 @default.
- W2136094884 hasConceptScore W2136094884C185592680 @default.
- W2136094884 hasConceptScore W2136094884C202751555 @default.
- W2136094884 hasConceptScore W2136094884C2781403057 @default.
- W2136094884 hasConceptScore W2136094884C38349280 @default.
- W2136094884 hasConceptScore W2136094884C55493867 @default.
- W2136094884 hasConceptScore W2136094884C57879066 @default.
- W2136094884 hasConceptScore W2136094884C86803240 @default.
- W2136094884 hasConceptScore W2136094884C90278072 @default.
- W2136094884 hasIssue "1" @default.
- W2136094884 hasLocation W21360948841 @default.
- W2136094884 hasLocation W21360948842 @default.
- W2136094884 hasOpenAccess W2136094884 @default.
- W2136094884 hasPrimaryLocation W21360948841 @default.
- W2136094884 hasRelatedWork W1991171247 @default.
- W2136094884 hasRelatedWork W2048682860 @default.
- W2136094884 hasRelatedWork W2050535146 @default.
- W2136094884 hasRelatedWork W2066384278 @default.
- W2136094884 hasRelatedWork W2080161812 @default.
- W2136094884 hasRelatedWork W2083818491 @default.
- W2136094884 hasRelatedWork W2364098574 @default.
- W2136094884 hasRelatedWork W2389468632 @default.
- W2136094884 hasRelatedWork W2995762087 @default.
- W2136094884 hasRelatedWork W4287994898 @default.
- W2136094884 hasVolume "34" @default.
- W2136094884 isParatext "false" @default.
- W2136094884 isRetracted "false" @default.
- W2136094884 magId "2136094884" @default.
- W2136094884 workType "article" @default.