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• W2136111703 abstract "SUMMARY The DNA of Autographa californica nuclear polyhedrosis virus (AcNPV) was isolated from extracellular non-occluded virions. The guanosine plus cytosine content of AcNPV DNA was estimated from its buoyant density in CsCl and its Tm value to be about 43 mol %. In ethidium bromide-CsCl gradients double-stranded covalently closed DNA molecules (form I) were detected, while relaxed circular (form II) and linear (form III) DNA molecules were demonstrated by velocity sedimentation in sucrose. The mol. wt. of AcNPV DNA was calculated to be about 78 × 106 from the sedimentation value of form III DNA. Digestion of AcNPV DNA with restriction endonucleases EcoRI and BamI resulted in 21 and 7 fragments, respectively, after analysis on agarose gels. The mol. wt. of the fragments and their molar ratio were determined. By this method the mol. wt. of AcNPV DNA was calculated to be about 83 × 106." @default.
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• W2136111703 date "1979-08-01" @default.
• W2136111703 modified "2023-10-17" @default.
• W2136111703 title "Characterization of Autographa californica Nuclear Polyhedrosis Virus Deoxyribonucleic Acid" @default.
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• W2136111703 doi "https://doi.org/10.1099/0022-1317-44-2-333" @default.
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