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- W2136128097 abstract "An enzyme fraction from aged swede root disks catalyses the formation of CoA thioesters of cinnamic acids in the presence of CoA, ATP and Mg2+. The enzyme shows activity only to those cinnamic acid derivatives bearing a phenolic OH group, p-coumaric and ferulic acids being the most active substrates. The requirement for Mg2+ can be replaced by Mn2+, Co2+ or Ni2+. The requirement for ATP could not be replaced by GTP, CTP, UTP, ADP or AMP. ADP and AMP, but not pyrophosphate, inhibited the ATP dependent activation of p-coumarate. The activity was inhibited by N-ethylmaleimide and p-chloro-mercuribenzoate which suggests a requirement for -SH groups for activation. The activity of the enzyme is low in freshly prepared disks but rises during ageing, particularly if the ageing is carried out in the presence of low concentrations of ethylene." @default.
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- W2136128097 date "1973-10-01" @default.
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- W2136128097 title "Formation of coA esters of cinnamic acid derivatives by extracts of Brassica napo-brassica root tissue" @default.
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- W2136128097 doi "https://doi.org/10.1016/0031-9422(73)80443-1" @default.
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