Matches in SemOpenAlex for { <https://semopenalex.org/work/W2136170436> ?p ?o ?g. }
- W2136170436 endingPage "53" @default.
- W2136170436 startingPage "38" @default.
- W2136170436 abstract "Abstract Methylthioalkylmalate synthase (MAM) catalyzes the committed step in the side chain elongation of Met, yielding important precursors for glucosinolate biosynthesis in Arabidopsis thaliana and other Brassicaceae species. MAM is believed to have evolved from isopropylmalate synthase (IPMS), an enzyme involved in Leu biosynthesis, based on phylogenetic analyses and an overlap of catalytic abilities. Here, we investigated the changes in protein structure that have occurred during the recruitment of IPMS from amino acid to glucosinolate metabolism. The major sequence difference between IPMS and MAM is the absence of 120 amino acids at the C-terminal end of MAM that constitute a regulatory domain for Leu-mediated feedback inhibition. Truncation of this domain in Arabidopsis IPMS2 results in loss of Leu feedback inhibition and quaternary structure, two features common to MAM enzymes, plus an 8.4-fold increase in the k cat/K m for a MAM substrate. Additional exchange of two amino acids in the active site resulted in a MAM-like enzyme that had little residual IPMS activity. Hence, combination of the loss of the regulatory domain and a few additional amino acid exchanges can explain the evolution of MAM from IPMS during its recruitment from primary to secondary metabolism." @default.
- W2136170436 created "2016-06-24" @default.
- W2136170436 creator A5021367666 @default.
- W2136170436 creator A5071511664 @default.
- W2136170436 date "2011-01-01" @default.
- W2136170436 modified "2023-10-16" @default.
- W2136170436 title "From Amino Acid to Glucosinolate Biosynthesis: Protein Sequence Changes in the Evolution of Methylthioalkylmalate Synthase in <i>Arabidopsis</i> " @default.
- W2136170436 cites W1857572381 @default.
- W2136170436 cites W1917335220 @default.
- W2136170436 cites W1963675471 @default.
- W2136170436 cites W1974719543 @default.
- W2136170436 cites W1985629904 @default.
- W2136170436 cites W1986111972 @default.
- W2136170436 cites W1991818122 @default.
- W2136170436 cites W1992495932 @default.
- W2136170436 cites W1996073320 @default.
- W2136170436 cites W1998591058 @default.
- W2136170436 cites W2011121716 @default.
- W2136170436 cites W2011254446 @default.
- W2136170436 cites W2018599255 @default.
- W2136170436 cites W2026032273 @default.
- W2136170436 cites W2031583546 @default.
- W2136170436 cites W2038629676 @default.
- W2136170436 cites W2044121564 @default.
- W2136170436 cites W2044854256 @default.
- W2136170436 cites W2050403202 @default.
- W2136170436 cites W2062541459 @default.
- W2136170436 cites W2062663912 @default.
- W2136170436 cites W2074132351 @default.
- W2136170436 cites W2080675830 @default.
- W2136170436 cites W2081912151 @default.
- W2136170436 cites W2083945806 @default.
- W2136170436 cites W2091222115 @default.
- W2136170436 cites W2094363695 @default.
- W2136170436 cites W2099299146 @default.
- W2136170436 cites W2108751174 @default.
- W2136170436 cites W2110224836 @default.
- W2136170436 cites W2111561341 @default.
- W2136170436 cites W2117538889 @default.
- W2136170436 cites W2119606683 @default.
- W2136170436 cites W2124397449 @default.
- W2136170436 cites W2127648442 @default.
- W2136170436 cites W2129095580 @default.
- W2136170436 cites W2133174791 @default.
- W2136170436 cites W2140189582 @default.
- W2136170436 cites W2142408194 @default.
- W2136170436 cites W2145985152 @default.
- W2136170436 cites W2153702005 @default.
- W2136170436 cites W2162470841 @default.
- W2136170436 cites W2171721177 @default.
- W2136170436 cites W2172276499 @default.
- W2136170436 cites W2312458836 @default.
- W2136170436 cites W4229487902 @default.
- W2136170436 doi "https://doi.org/10.1105/tpc.110.079269" @default.
- W2136170436 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3051243" @default.
- W2136170436 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21205930" @default.
- W2136170436 hasPublicationYear "2011" @default.
- W2136170436 type Work @default.
- W2136170436 sameAs 2136170436 @default.
- W2136170436 citedByCount "84" @default.
- W2136170436 countsByYear W21361704362012 @default.
- W2136170436 countsByYear W21361704362013 @default.
- W2136170436 countsByYear W21361704362014 @default.
- W2136170436 countsByYear W21361704362015 @default.
- W2136170436 countsByYear W21361704362016 @default.
- W2136170436 countsByYear W21361704362017 @default.
- W2136170436 countsByYear W21361704362018 @default.
- W2136170436 countsByYear W21361704362019 @default.
- W2136170436 countsByYear W21361704362020 @default.
- W2136170436 countsByYear W21361704362021 @default.
- W2136170436 countsByYear W21361704362022 @default.
- W2136170436 countsByYear W21361704362023 @default.
- W2136170436 crossrefType "journal-article" @default.
- W2136170436 hasAuthorship W2136170436A5021367666 @default.
- W2136170436 hasAuthorship W2136170436A5071511664 @default.
- W2136170436 hasBestOaLocation W21361704361 @default.
- W2136170436 hasConcept C104317684 @default.
- W2136170436 hasConcept C112243037 @default.
- W2136170436 hasConcept C113850282 @default.
- W2136170436 hasConcept C143065580 @default.
- W2136170436 hasConcept C167625842 @default.
- W2136170436 hasConcept C181199279 @default.
- W2136170436 hasConcept C2775988993 @default.
- W2136170436 hasConcept C2776747608 @default.
- W2136170436 hasConcept C2778164613 @default.
- W2136170436 hasConcept C2779491563 @default.
- W2136170436 hasConcept C515207424 @default.
- W2136170436 hasConcept C553450214 @default.
- W2136170436 hasConcept C55493867 @default.
- W2136170436 hasConcept C59822182 @default.
- W2136170436 hasConcept C86803240 @default.
- W2136170436 hasConceptScore W2136170436C104317684 @default.
- W2136170436 hasConceptScore W2136170436C112243037 @default.
- W2136170436 hasConceptScore W2136170436C113850282 @default.
- W2136170436 hasConceptScore W2136170436C143065580 @default.
- W2136170436 hasConceptScore W2136170436C167625842 @default.
- W2136170436 hasConceptScore W2136170436C181199279 @default.
- W2136170436 hasConceptScore W2136170436C2775988993 @default.