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• W2136176791 abstract "Shoot-to-landscape scale sources of variation in photosynthetic characteristics of Thalassia testudinum , as measured in situ using a submersible pulse-amplitude modulated fluorometer (diving-PAM), were investigated. Shoot-scale variation was assessed to develop a standard-methods protocol for this species. Significant within-shoot and among-shoot scale variation was observed for several PAM-fluorescence parameters. The fraction of incident photosynthetically active radiation (PAR) absorbed by rank 1 (youngest) leaves, 0.67±0.03, was significantly lower than the PAR absorbed by rank 2 and 3 leaves (0.78±0.04 and 0.77±0.04, respectively). Quantum yields ( Y ) and photosynthetic efficiency ( F v / F m ) exhibited greater variability and generally decreased with increasing leaf age ( Y =0.78±0.02, 0.78±0.03, and 0.76±0.06 and F v / F m =0.79±0.02, 0.77±0.5, and 0.75±0.06 for rank 1, 2, and 3 leaves, respectively). Maximum fluorescence of light-acclimated leaves ( F ′ m ) significantly decreased from the base to the tip of leaves (1032±203, 1059±139, and 793±107 for the base, middle and tip of the leaf) and Y was significantly reduced from 0.68–0.76 to 0.27–0.62 when measured where leaf lesions were present. However, paired comparisons (with versus without lesions at the tip of the leaf) for the base, middle, and tip areas of rank 2 leaves indicated that lesions effects on photosynthesis were localized only to the area of the lesion (tip). The Y exhibited relatively high variability for short-shoots within seagrass die-off patches and were significantly reduced for short-shoots exhibiting die-off symptoms (rotten leaf base grayish-green leaf color, but no lesions). Negative response slopes for Y and F v / F m , measured as part of a landscape-scale sampling program, revealed the presence of significant diurnal (time-of-day) and photosynthetic photon flux density (PPFD) related variation, with a more negative slope for Y . These results indicate that the selection of leaf tissue, short-shoot location, and time of measurement need to be considered when determining photosynthetic rates for seagrasses in situ." @default.
• W2136176791 created "2016-06-24" @default.
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• W2136176791 date "2002-06-01" @default.
• W2136176791 modified "2023-09-26" @default.
• W2136176791 title "Photosynthetic characteristics of Thalassia testudinum measured in situ by pulse-amplitude modulated (PAM) fluorometry: methodological and scale-based considerations" @default.
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• W2136176791 doi "https://doi.org/10.1016/s0304-3770(02)00020-7" @default.
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