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• W2136177148 abstract "Existe atualmente grande preocupacao com a melhoria no diagnostico da infeccao pelo Papilomavirus Humano (HPV), poiseste virus tem sido responsavel pela principal doenca sexualmente transmissivel de etiologia viral e apresenta correlacao comos processos malignos e lesoes precursoras em cervice uterina. Neste trabalho sao apresentadas as principais tecnicas dedeteccao molecular para o diagnostico do HPV atualmente disponiveis, como a PCR (Reacao em Cadeia da Polimerase), aCaptura Hibrida e a Hibridizacao In Situ, com maior enfase na PCR. E tambem discutida a sensibilidade das tecnicasmoleculares como PCR e PCR em Tempo Real para deteccao destes virus nos tecidos epiteliais antes mesmo doaparecimento de lesoes ou sintomas. Com este trabalho foi possivel verificar que a tecnica de PCR, em relacao aos demaistestes disponiveis, tem uma maior sensibilidade, sendo capaz de detectar uma reduzida quantidade de amostras e tambempermitir a verificacao de infeccoes por multiplos subtipos, bem como avaliar o risco oncogenico da populacao viral infectante. PRINCIPAL MOLECULAR THECNIQUES FOR DETECTING HUMAN PAPILLOMAVIRUS Abstract Nowadays, there is a great concern with the development of HPV (Human Papillomavirus) virus infection diagnosis. HPV virushas been responsible for main sexually transmissible diseases of viral etiology and shows correlation with malignant processesand precursory lesions in uterine cervix. This research shows the main actually available techniques of molecular detection forHPV diagnosis, as PCR (Polymerase Chain Reaction), Hybrid Capture and In Situ Hibridization, giving emphasis to PCR. Italso discusses about the sensibility of molecular assays as PCR and Real Time PCR for HPV detection in epithelial tissuesbefore the occurrence of lesions or symptoms. This study has shown that PCR technique, when compared to other availabledetection tests, has superior sensibility. This technique is also able to detect a reduced amount of samples and it allows theverification of infections by multiple virus subtypes, as well as the evaluation of oncogenic risk of viral infecting population." @default.
• W2136177148 created "2016-06-24" @default.
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• W2136177148 date "2007-06-30" @default.
• W2136177148 modified "2023-09-23" @default.
• W2136177148 title "PRINCIPAIS TÉCNICAS MOLECULARES PARA DETECÇÃO DO PAPILOMAVÍRUS HUMANO" @default.
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