Matches in SemOpenAlex for { <https://semopenalex.org/work/W2136193090> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2136193090 endingPage "284" @default.
- W2136193090 startingPage "282" @default.
- W2136193090 abstract "The speA gene in Bacillus subtilis encodes arginine decarboxylase, which catalyzes the conversion of arginine to agmatine. Arginine decarboxylase is an important enzyme in polyamine metabolism in B. subtilis. In order to further illustrate the catalytic mechanism of arginine decarboxylase by determining the three-dimensional structure of the enzyme, the speA gene was amplified from B. subtilis genomic DNA and cloned into the expression vector pET-28a(+). SpeA was expressed in Escherichia coli and purified to homogeneity by nickel-chelation chromatography followed by size-exclusion chromatography. High-quality crystals were obtained using the hanging-drop vapour-diffusion method at 289 K. The best crystal diffracted to 2.0 Å resolution and belonged to space group P21, with unit-cell parameters a = 86.4, b = 63.3 c = 103.3 Å, β = 113.9°." @default.
- W2136193090 created "2016-06-24" @default.
- W2136193090 creator A5008071965 @default.
- W2136193090 creator A5055302562 @default.
- W2136193090 creator A5056448190 @default.
- W2136193090 creator A5056478201 @default.
- W2136193090 creator A5071446370 @default.
- W2136193090 date "2009-02-26" @default.
- W2136193090 modified "2023-10-16" @default.
- W2136193090 title "Preliminary X-ray crystallographic studies of<i>Bacillus subtilis</i>SpeA protein" @default.
- W2136193090 cites W1501927495 @default.
- W2136193090 cites W1534584261 @default.
- W2136193090 cites W1541698544 @default.
- W2136193090 cites W1548489352 @default.
- W2136193090 cites W1578349954 @default.
- W2136193090 cites W1890299681 @default.
- W2136193090 cites W1987022733 @default.
- W2136193090 cites W2000840258 @default.
- W2136193090 cites W2011682073 @default.
- W2136193090 cites W2014694459 @default.
- W2136193090 cites W2032118018 @default.
- W2136193090 cites W2033719772 @default.
- W2136193090 cites W2060658515 @default.
- W2136193090 cites W2103084013 @default.
- W2136193090 cites W2106882534 @default.
- W2136193090 doi "https://doi.org/10.1107/s1744309109003856" @default.
- W2136193090 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2650450" @default.
- W2136193090 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19255484" @default.
- W2136193090 hasPublicationYear "2009" @default.
- W2136193090 type Work @default.
- W2136193090 sameAs 2136193090 @default.
- W2136193090 citedByCount "1" @default.
- W2136193090 crossrefType "journal-article" @default.
- W2136193090 hasAuthorship W2136193090A5008071965 @default.
- W2136193090 hasAuthorship W2136193090A5055302562 @default.
- W2136193090 hasAuthorship W2136193090A5056448190 @default.
- W2136193090 hasAuthorship W2136193090A5056478201 @default.
- W2136193090 hasAuthorship W2136193090A5071446370 @default.
- W2136193090 hasBestOaLocation W21361930902 @default.
- W2136193090 hasConcept C104317684 @default.
- W2136193090 hasConcept C153911025 @default.
- W2136193090 hasConcept C181199279 @default.
- W2136193090 hasConcept C185592680 @default.
- W2136193090 hasConcept C2777107663 @default.
- W2136193090 hasConcept C2777167505 @default.
- W2136193090 hasConcept C2777272437 @default.
- W2136193090 hasConcept C2777468819 @default.
- W2136193090 hasConcept C2781448169 @default.
- W2136193090 hasConcept C515207424 @default.
- W2136193090 hasConcept C523546767 @default.
- W2136193090 hasConcept C54355233 @default.
- W2136193090 hasConcept C547475151 @default.
- W2136193090 hasConcept C55493867 @default.
- W2136193090 hasConcept C86803240 @default.
- W2136193090 hasConceptScore W2136193090C104317684 @default.
- W2136193090 hasConceptScore W2136193090C153911025 @default.
- W2136193090 hasConceptScore W2136193090C181199279 @default.
- W2136193090 hasConceptScore W2136193090C185592680 @default.
- W2136193090 hasConceptScore W2136193090C2777107663 @default.
- W2136193090 hasConceptScore W2136193090C2777167505 @default.
- W2136193090 hasConceptScore W2136193090C2777272437 @default.
- W2136193090 hasConceptScore W2136193090C2777468819 @default.
- W2136193090 hasConceptScore W2136193090C2781448169 @default.
- W2136193090 hasConceptScore W2136193090C515207424 @default.
- W2136193090 hasConceptScore W2136193090C523546767 @default.
- W2136193090 hasConceptScore W2136193090C54355233 @default.
- W2136193090 hasConceptScore W2136193090C547475151 @default.
- W2136193090 hasConceptScore W2136193090C55493867 @default.
- W2136193090 hasConceptScore W2136193090C86803240 @default.
- W2136193090 hasIssue "3" @default.
- W2136193090 hasLocation W21361930901 @default.
- W2136193090 hasLocation W21361930902 @default.
- W2136193090 hasLocation W21361930903 @default.
- W2136193090 hasLocation W21361930904 @default.
- W2136193090 hasOpenAccess W2136193090 @default.
- W2136193090 hasPrimaryLocation W21361930901 @default.
- W2136193090 hasRelatedWork W1929032322 @default.
- W2136193090 hasRelatedWork W1970959157 @default.
- W2136193090 hasRelatedWork W1991929655 @default.
- W2136193090 hasRelatedWork W2044630163 @default.
- W2136193090 hasRelatedWork W2081220827 @default.
- W2136193090 hasRelatedWork W2157739137 @default.
- W2136193090 hasRelatedWork W2272183236 @default.
- W2136193090 hasRelatedWork W2408067028 @default.
- W2136193090 hasRelatedWork W2413124027 @default.
- W2136193090 hasRelatedWork W80307186 @default.
- W2136193090 hasVolume "65" @default.
- W2136193090 isParatext "false" @default.
- W2136193090 isRetracted "false" @default.
- W2136193090 magId "2136193090" @default.
- W2136193090 workType "article" @default.