Matches in SemOpenAlex for { <https://semopenalex.org/work/W2136291211> ?p ?o ?g. }
- W2136291211 endingPage "133" @default.
- W2136291211 startingPage "117" @default.
- W2136291211 abstract "Cohesin complex mediates cohesion between sister chromatids, which promotes high-fidelity chromosome segregation. Eco1p acetylates the cohesin subunit Smc3p during S phase to establish cohesion. The current model posits that this Eco1p-mediated acetylation promotes establishment by abrogating the ability of Wpl1p to destabilize cohesin binding to chromosomes. Here we present data from budding yeast that is incompatible with this Wpl1p-centric model. Two independent in vivo assays show that a wpl1∆ fails to suppress cohesion defects of eco1∆ cells. Moreover, a wpl1∆ also fails to suppress cohesion defects engendered by blocking just the essential Eco1p acetylation sites on Smc3p (K112, K113). Thus removing WPL1 inhibition is insufficient for generating cohesion without ECO1 activity. To elucidate how ECO1 promotes cohesion, we conducted a genetic screen and identified a cohesion activator mutation in the SMC3 head domain (D1189H). Smc3-D1189H partially restores cohesion in eco1∆ wpl1∆ or eco1 mutant cells but robustly restores cohesion in cells blocked for Smc3p K112 K113 acetylation. These data support two important conclusions. First, acetylation of the K112 K113 region by Eco1p promotes cohesion establishment by altering Smc3p head function independent of its ability to antagonize Wpl1p. Second, Eco1p targets other than Smc3p K112 K113 are necessary for efficient establishment." @default.
- W2136291211 created "2016-06-24" @default.
- W2136291211 creator A5015949250 @default.
- W2136291211 creator A5016754074 @default.
- W2136291211 creator A5023672246 @default.
- W2136291211 creator A5031917433 @default.
- W2136291211 creator A5040167004 @default.
- W2136291211 creator A5078027494 @default.
- W2136291211 date "2015-01-01" @default.
- W2136291211 modified "2023-10-06" @default.
- W2136291211 title "A novel mechanism for the establishment of sister chromatid cohesion by the <i>ECO1</i> acetyltransferase" @default.
- W2136291211 cites W1963648581 @default.
- W2136291211 cites W1966900987 @default.
- W2136291211 cites W1969497434 @default.
- W2136291211 cites W1971799079 @default.
- W2136291211 cites W1972307085 @default.
- W2136291211 cites W1972918915 @default.
- W2136291211 cites W1974930724 @default.
- W2136291211 cites W1978696228 @default.
- W2136291211 cites W1979162302 @default.
- W2136291211 cites W1979298341 @default.
- W2136291211 cites W1980236727 @default.
- W2136291211 cites W1984637914 @default.
- W2136291211 cites W1992117836 @default.
- W2136291211 cites W1998346523 @default.
- W2136291211 cites W2003101935 @default.
- W2136291211 cites W2004304977 @default.
- W2136291211 cites W2007093272 @default.
- W2136291211 cites W2015957073 @default.
- W2136291211 cites W2021859989 @default.
- W2136291211 cites W2027329763 @default.
- W2136291211 cites W2038178643 @default.
- W2136291211 cites W2040838669 @default.
- W2136291211 cites W2049297352 @default.
- W2136291211 cites W2052648473 @default.
- W2136291211 cites W2067471138 @default.
- W2136291211 cites W2069721588 @default.
- W2136291211 cites W2088509777 @default.
- W2136291211 cites W2089736577 @default.
- W2136291211 cites W2091502861 @default.
- W2136291211 cites W2095639780 @default.
- W2136291211 cites W2097201830 @default.
- W2136291211 cites W2099943527 @default.
- W2136291211 cites W2106624058 @default.
- W2136291211 cites W2120917736 @default.
- W2136291211 cites W2120918097 @default.
- W2136291211 cites W2121056577 @default.
- W2136291211 cites W2122997931 @default.
- W2136291211 cites W2126064683 @default.
- W2136291211 cites W2133188555 @default.
- W2136291211 cites W2133492463 @default.
- W2136291211 cites W2137435947 @default.
- W2136291211 cites W2139114666 @default.
- W2136291211 cites W2139189803 @default.
- W2136291211 cites W2142157138 @default.
- W2136291211 cites W2143157366 @default.
- W2136291211 cites W2145020264 @default.
- W2136291211 cites W2149141811 @default.
- W2136291211 cites W2152414953 @default.
- W2136291211 cites W2153882574 @default.
- W2136291211 cites W2159938983 @default.
- W2136291211 doi "https://doi.org/10.1091/mbc.e14-08-1268" @default.
- W2136291211 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4279223" @default.
- W2136291211 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25378582" @default.
- W2136291211 hasPublicationYear "2015" @default.
- W2136291211 type Work @default.
- W2136291211 sameAs 2136291211 @default.
- W2136291211 citedByCount "33" @default.
- W2136291211 countsByYear W21362912112015 @default.
- W2136291211 countsByYear W21362912112016 @default.
- W2136291211 countsByYear W21362912112017 @default.
- W2136291211 countsByYear W21362912112018 @default.
- W2136291211 countsByYear W21362912112019 @default.
- W2136291211 countsByYear W21362912112020 @default.
- W2136291211 countsByYear W21362912112021 @default.
- W2136291211 countsByYear W21362912112022 @default.
- W2136291211 countsByYear W21362912112023 @default.
- W2136291211 crossrefType "journal-article" @default.
- W2136291211 hasAuthorship W2136291211A5015949250 @default.
- W2136291211 hasAuthorship W2136291211A5016754074 @default.
- W2136291211 hasAuthorship W2136291211A5023672246 @default.
- W2136291211 hasAuthorship W2136291211A5031917433 @default.
- W2136291211 hasAuthorship W2136291211A5040167004 @default.
- W2136291211 hasAuthorship W2136291211A5078027494 @default.
- W2136291211 hasBestOaLocation W21362912111 @default.
- W2136291211 hasConcept C104317684 @default.
- W2136291211 hasConcept C115821613 @default.
- W2136291211 hasConcept C119157956 @default.
- W2136291211 hasConcept C148196450 @default.
- W2136291211 hasConcept C152369539 @default.
- W2136291211 hasConcept C2778354886 @default.
- W2136291211 hasConcept C2779649592 @default.
- W2136291211 hasConcept C30481170 @default.
- W2136291211 hasConcept C54355233 @default.
- W2136291211 hasConcept C73397532 @default.
- W2136291211 hasConcept C86803240 @default.
- W2136291211 hasConcept C95444343 @default.
- W2136291211 hasConceptScore W2136291211C104317684 @default.