FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2136303496> ?p ?o ?g. }

• W2136303496 endingPage "10132" @default.
• W2136303496 startingPage "10122" @default.
• W2136303496 abstract "ABSTRACT During infection, adenovirus (Ad) capsids undergo microtubule-dependent retrograde transport as part of a program of vectorial transport of the viral genome to the nucleus. The microtubule-associated molecular motor, cytoplasmic dynein, has been implicated in the retrograde movement of Ad. We hypothesized that cytoplasmic dynein constituted the primary mode of association of Ad with microtubules. To evaluate this hypothesis, an Ad-microtubule binding assay was established in which microtubules were polymerized with taxol, combined with Ad in the presence or absence of microtubule-associated proteins (MAPs), and centrifuged through a glycerol cushion. The addition of purified bovine brain MAPs increased the fraction of Ad in the microtubule pellet from 17.3% ± 3.5% to 80.7% ± 3.8% ( P < 0.01). In the absence of tubulin polymerization or in the presence of high salt, no Ad was found in the pellet. Ad binding to microtubules was not enhanced by bovine brain MAPs enriched for tau protein or by the addition of bovine serum albumin. Enhanced Ad-microtubule binding was also observed by using a fraction of MAPs purified from lung A549 epithelial cell lysate which contained cytoplasmic dynein. Ad-microtubule interaction was sensitive to the addition of ATP, a hallmark of cytoplasmic dynein-dependent microtubule interactions. Immunodepletion of cytoplasmic dynein from the A549 cell lysate abolished the MAP-enhanced Ad-microtubule binding. The interaction of Ad with both dynein and dynactin complexes was demonstrated by coimmunoprecipitation. Partially uncoated capsids isolated from cells 40 min after infection also exhibited microtubule binding. In summary, the primary mode of Ad attachment to microtubules occurs though cytoplasmic dynein-mediated binding." @default.
• W2136303496 created "2016-06-24" @default.
• W2136303496 creator A5027155026 @default.
• W2136303496 creator A5049921479 @default.
• W2136303496 creator A5057768807 @default.
• W2136303496 creator A5064433832 @default.
• W2136303496 date "2004-09-15" @default.
• W2136303496 modified "2023-10-16" @default.
• W2136303496 title "Cytoplasmic Dynein Mediates Adenovirus Binding to Microtubules" @default.
• W2136303496 cites W1486052360 @default.
• W2136303496 cites W1496507358 @default.
• W2136303496 cites W1508340724 @default.
• W2136303496 cites W1536783702 @default.
• W2136303496 cites W1554847983 @default.
• W2136303496 cites W1740655975 @default.
• W2136303496 cites W18142307 @default.
• W2136303496 cites W1850839198 @default.
• W2136303496 cites W1856278699 @default.
• W2136303496 cites W1857650329 @default.
• W2136303496 cites W1928181584 @default.
• W2136303496 cites W1967402467 @default.
• W2136303496 cites W1968924490 @default.
• W2136303496 cites W1969945645 @default.
• W2136303496 cites W1970182993 @default.
• W2136303496 cites W1980401440 @default.
• W2136303496 cites W1981517627 @default.
• W2136303496 cites W1982275607 @default.
• W2136303496 cites W1985151525 @default.
• W2136303496 cites W1986511337 @default.
• W2136303496 cites W1989498124 @default.
• W2136303496 cites W1989547495 @default.
• W2136303496 cites W1991422862 @default.
• W2136303496 cites W1997629300 @default.
• W2136303496 cites W2001860659 @default.
• W2136303496 cites W2012401859 @default.
• W2136303496 cites W2012660700 @default.
• W2136303496 cites W2013377057 @default.
• W2136303496 cites W2025129187 @default.
• W2136303496 cites W2027724937 @default.
• W2136303496 cites W2031306380 @default.
• W2136303496 cites W2032342779 @default.
• W2136303496 cites W2034028084 @default.
• W2136303496 cites W2035644668 @default.
• W2136303496 cites W2040238933 @default.
• W2136303496 cites W2041580060 @default.
• W2136303496 cites W2045724063 @default.
• W2136303496 cites W2046218623 @default.
• W2136303496 cites W2046928671 @default.
• W2136303496 cites W2048782201 @default.
• W2136303496 cites W2051758487 @default.
• W2136303496 cites W2052399367 @default.
• W2136303496 cites W2056061056 @default.
• W2136303496 cites W2059055698 @default.
• W2136303496 cites W2061744433 @default.
• W2136303496 cites W2065392104 @default.
• W2136303496 cites W2071577667 @default.
• W2136303496 cites W2072170340 @default.
• W2136303496 cites W2073273867 @default.
• W2136303496 cites W2077541686 @default.
• W2136303496 cites W2082631326 @default.
• W2136303496 cites W2085001709 @default.
• W2136303496 cites W2088952492 @default.
• W2136303496 cites W2089620930 @default.
• W2136303496 cites W2094240337 @default.
• W2136303496 cites W2098264302 @default.
• W2136303496 cites W2100248438 @default.
• W2136303496 cites W2103617829 @default.
• W2136303496 cites W2111754083 @default.
• W2136303496 cites W2113046511 @default.
• W2136303496 cites W2114392102 @default.
• W2136303496 cites W2114697595 @default.
• W2136303496 cites W2116071714 @default.
• W2136303496 cites W2118642774 @default.
• W2136303496 cites W2122280772 @default.
• W2136303496 cites W2129333552 @default.
• W2136303496 cites W2133388585 @default.
• W2136303496 cites W2137115293 @default.
• W2136303496 cites W2139741312 @default.
• W2136303496 cites W2140362030 @default.
• W2136303496 cites W2143634143 @default.
• W2136303496 cites W2144301785 @default.
• W2136303496 cites W2145479669 @default.
• W2136303496 cites W2151525547 @default.
• W2136303496 cites W2162530599 @default.
• W2136303496 cites W2168637323 @default.
• W2136303496 cites W2168733588 @default.
• W2136303496 cites W2170540823 @default.
• W2136303496 cites W2173136445 @default.
• W2136303496 cites W2173520530 @default.
• W2136303496 cites W2335675667 @default.
• W2136303496 cites W2410783912 @default.
• W2136303496 cites W824328 @default.
• W2136303496 doi "https://doi.org/10.1128/jvi.78.18.10122-10132.2004" @default.
• W2136303496 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/515014" @default.
• W2136303496 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15331745" @default.
• W2136303496 hasPublicationYear "2004" @default.
• W2136303496 type Work @default.
• W2136303496 sameAs 2136303496 @default.

• next