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• W2136407088 abstract "Methods are described for the detection of low numbers of bacteria by monitoring 14 CO 2 evolved from 14 C-labeled substrates. Cell suspensions are filtered with membrane filters, and the filter is then moistened with 0.1 ml of labeled medium in a small, closed apparatus. Evolved 14 CO 2 is collected with Ba(OH) 2 -moistened filter pads and assayed with conventional radioactivity counting equipment. The kinetics of 14 CO 2 evolution are shown for several species of bacteria. Fewer than 100 colony-forming units of most species tested were detected in 2 h or less. Bacteria were inoculated into blood and the mixture was treated to lyse the blood cells. The suspension ws filtered and the filter was placed in a small volume of labeled medium. The evolved 14 CO 2 was trapped and counted. A key development in the methodology was finding that an aqueous solution of Rhyozyme and Triton X-100 produced lysis of blood but was not detrimental to bacteria." @default.
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• W2136407088 date "1973-12-01" @default.
• W2136407088 modified "2023-10-04" @default.
• W2136407088 title "Method for Radiorespirometric Detection of Bacteria in Pure Culture and in Blood" @default.
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• W2136407088 doi "https://doi.org/10.1128/am.26.6.867-873.1973" @default.
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