Matches in SemOpenAlex for { <https://semopenalex.org/work/W2136477215> ?p ?o ?g. }
- W2136477215 endingPage "689" @default.
- W2136477215 startingPage "684" @default.
- W2136477215 abstract "Macrolide-resistant Helicobacter (H.) pylori represent an increasing therapeutic problem. Macrolide resistance is usually determined phenotypically in vitro with methods such as E-test or agar dilution test. A prerequisite for those tests, however, is bacterial culture that is not routinely set up in the course of gastroscopy. In contrast, formalin-fixed, paraffin-embedded biopsies are regularly available from patients who have undergone gastroscopy. In such biopsies macrolide-resistant H. pylori can be detected by the genotype-based technique of fluorescence in situ hybridization (FISH). Experience gained by this new method, however, is still extremely limited, especially in formalin-fixed tissue. Therefore, we retrospectively investigated formalin-fixed, paraffin-embedded biopsy specimens by FISH in 104 patients suffering from therapy-resistant H. pylori gastritis. To test the accuracy of FISH, we initially examined specimens from 53 patients for whom results of the E-test were available. Next we analyzed biopsies from another 51 patients that had been selected since phenotypical resistance testing had failed despite documented culturing attempts. In all 104 patients, H. pylori was detected by FISH and could thus be investigated for macrolide resistance. Overall, macrolide-resistant bacteria were found in 71 patients (68.3%). In 49 of 53 patients (92.4%), FISH and E-test returned identical results (no significant discordance according to McNemar's chi(2)-test). Taken together, our study demonstrates that FISH is a highly sensitive and reliable method for detecting macrolide-resistant H. pylori in formalin-fixed, paraffin-embedded biopsy specimens, which represents the routine method of processing tissue obtained upon gastroscopy." @default.
- W2136477215 created "2016-06-24" @default.
- W2136477215 creator A5008211775 @default.
- W2136477215 creator A5012621209 @default.
- W2136477215 creator A5014931098 @default.
- W2136477215 creator A5015723785 @default.
- W2136477215 creator A5054331215 @default.
- W2136477215 creator A5068561527 @default.
- W2136477215 creator A5089235164 @default.
- W2136477215 creator A5091851420 @default.
- W2136477215 date "2004-06-01" @default.
- W2136477215 modified "2023-10-03" @default.
- W2136477215 title "Reliable detection of macrolide-resistant Helicobacter pylori via fluorescence in situ hybridization in formalin-fixed tissue" @default.
- W2136477215 cites W1486801058 @default.
- W2136477215 cites W1561069887 @default.
- W2136477215 cites W1573186537 @default.
- W2136477215 cites W159526248 @default.
- W2136477215 cites W1894963259 @default.
- W2136477215 cites W1910635526 @default.
- W2136477215 cites W1966111137 @default.
- W2136477215 cites W1974785123 @default.
- W2136477215 cites W2011985600 @default.
- W2136477215 cites W2025378336 @default.
- W2136477215 cites W2027425390 @default.
- W2136477215 cites W2034601791 @default.
- W2136477215 cites W2036229454 @default.
- W2136477215 cites W2038248809 @default.
- W2136477215 cites W2055577586 @default.
- W2136477215 cites W2061938579 @default.
- W2136477215 cites W2067126845 @default.
- W2136477215 cites W2070424942 @default.
- W2136477215 cites W2091705983 @default.
- W2136477215 cites W2092509407 @default.
- W2136477215 cites W2102181758 @default.
- W2136477215 cites W2105777613 @default.
- W2136477215 cites W2120719128 @default.
- W2136477215 cites W2123451607 @default.
- W2136477215 cites W2132600651 @default.
- W2136477215 cites W2135026519 @default.
- W2136477215 cites W2135406516 @default.
- W2136477215 cites W2136541410 @default.
- W2136477215 cites W2139657695 @default.
- W2136477215 cites W2142626267 @default.
- W2136477215 cites W2164497230 @default.
- W2136477215 cites W2167293183 @default.
- W2136477215 cites W2169501912 @default.
- W2136477215 cites W2948387191 @default.
- W2136477215 doi "https://doi.org/10.1038/modpathol.3800098" @default.
- W2136477215 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15044917" @default.
- W2136477215 hasPublicationYear "2004" @default.
- W2136477215 type Work @default.
- W2136477215 sameAs 2136477215 @default.
- W2136477215 citedByCount "32" @default.
- W2136477215 countsByYear W21364772152012 @default.
- W2136477215 countsByYear W21364772152014 @default.
- W2136477215 countsByYear W21364772152015 @default.
- W2136477215 countsByYear W21364772152016 @default.
- W2136477215 countsByYear W21364772152017 @default.
- W2136477215 countsByYear W21364772152018 @default.
- W2136477215 countsByYear W21364772152019 @default.
- W2136477215 countsByYear W21364772152021 @default.
- W2136477215 countsByYear W21364772152023 @default.
- W2136477215 crossrefType "journal-article" @default.
- W2136477215 hasAuthorship W2136477215A5008211775 @default.
- W2136477215 hasAuthorship W2136477215A5012621209 @default.
- W2136477215 hasAuthorship W2136477215A5014931098 @default.
- W2136477215 hasAuthorship W2136477215A5015723785 @default.
- W2136477215 hasAuthorship W2136477215A5054331215 @default.
- W2136477215 hasAuthorship W2136477215A5068561527 @default.
- W2136477215 hasAuthorship W2136477215A5089235164 @default.
- W2136477215 hasAuthorship W2136477215A5091851420 @default.
- W2136477215 hasBestOaLocation W21364772151 @default.
- W2136477215 hasConcept C104317684 @default.
- W2136477215 hasConcept C105795698 @default.
- W2136477215 hasConcept C126322002 @default.
- W2136477215 hasConcept C142724271 @default.
- W2136477215 hasConcept C186282968 @default.
- W2136477215 hasConcept C2775934546 @default.
- W2136477215 hasConcept C2776409635 @default.
- W2136477215 hasConcept C2777542201 @default.
- W2136477215 hasConcept C30481170 @default.
- W2136477215 hasConcept C33923547 @default.
- W2136477215 hasConcept C55493867 @default.
- W2136477215 hasConcept C71924100 @default.
- W2136477215 hasConcept C86803240 @default.
- W2136477215 hasConcept C89423630 @default.
- W2136477215 hasConceptScore W2136477215C104317684 @default.
- W2136477215 hasConceptScore W2136477215C105795698 @default.
- W2136477215 hasConceptScore W2136477215C126322002 @default.
- W2136477215 hasConceptScore W2136477215C142724271 @default.
- W2136477215 hasConceptScore W2136477215C186282968 @default.
- W2136477215 hasConceptScore W2136477215C2775934546 @default.
- W2136477215 hasConceptScore W2136477215C2776409635 @default.
- W2136477215 hasConceptScore W2136477215C2777542201 @default.
- W2136477215 hasConceptScore W2136477215C30481170 @default.
- W2136477215 hasConceptScore W2136477215C33923547 @default.
- W2136477215 hasConceptScore W2136477215C55493867 @default.
- W2136477215 hasConceptScore W2136477215C71924100 @default.