FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2136543503> ?p ?o ?g. }

• W2136543503 endingPage "736" @default.
• W2136543503 startingPage "727" @default.
• W2136543503 abstract "Interactions with local extracellular cues direct cell migration. A versatile method to study cell response to a protein consists of patterning the protein cue on a substrate and quantifying the distribution of cells between patterned and non-patterned areas. Here, we define the concepts of (i) cell–surface affinity to describe cell choices, and of (ii) reference surface (RS) to clarify that the choice is made relative to a reference. Furthermore, we report a method to systematically tune the RS and show that it can dominate the experimental cell response to a protein cue. The cell response to a cue can be switched from strong preference to strong aversion by only changing the RS. Using microcontact printing, we patterned the extracellular matrix proteins fibronectin or netrin-1 adjacent to a series of RSs with different ratios of poly-d-lysine (PDL) and polyethylene glycol (PEG), which are of high affinity and of low-affinity for cells, respectively. C2C12 myoblasts or primary neurons seeded on substrates with a high affinity RS (high % PDL) did not respond to a printed protein of interest, and conversely on RSs of low affinity (high % PEG) the cells preferred the printed protein even in the absence of a specific interaction. However, when testing cell response to a standardized series of RSs varying from high to low affinity, a specific response curve was obtained that was unique to each cell type–protein pair. Importantly, for intermediate RSs with moderate affinity, the cell response to the cue was dependent on the activation of biologically relevant protein-specific biochemical signal transduction pathways. Our results establish that choices made by cells in response to a surface-bound cue must take into account, and be interpreted in the context of, the RS. The use of a series of RSs with varying cell–surface affinity reveals specific response curves of cells to a cue that can be compared quantitatively and that may help gain new insights into cellular responses to extracellular proteins." @default.
• W2136543503 created "2016-06-24" @default.
• W2136543503 creator A5014037848 @default.
• W2136543503 creator A5016205291 @default.
• W2136543503 creator A5035701091 @default.
• W2136543503 creator A5069539980 @default.
• W2136543503 creator A5085811442 @default.
• W2136543503 date "2014-01-01" @default.
• W2136543503 modified "2023-10-16" @default.
• W2136543503 title "Tuning cell–surface affinity to direct cell specific responses to patterned proteins" @default.
• W2136543503 cites W110398411 @default.
• W2136543503 cites W149540474 @default.
• W2136543503 cites W1928800192 @default.
• W2136543503 cites W1964375590 @default.
• W2136543503 cites W1967060076 @default.
• W2136543503 cites W1967265143 @default.
• W2136543503 cites W1968421448 @default.
• W2136543503 cites W1977994901 @default.
• W2136543503 cites W1983491648 @default.
• W2136543503 cites W1987670066 @default.
• W2136543503 cites W1993006648 @default.
• W2136543503 cites W1997023256 @default.
• W2136543503 cites W2001091952 @default.
• W2136543503 cites W2003986026 @default.
• W2136543503 cites W2006695366 @default.
• W2136543503 cites W2009510402 @default.
• W2136543503 cites W2018723867 @default.
• W2136543503 cites W2018811490 @default.
• W2136543503 cites W2024239969 @default.
• W2136543503 cites W2026195220 @default.
• W2136543503 cites W2029351072 @default.
• W2136543503 cites W2031855642 @default.
• W2136543503 cites W2034301798 @default.
• W2136543503 cites W2040890530 @default.
• W2136543503 cites W2046968637 @default.
• W2136543503 cites W2048305477 @default.
• W2136543503 cites W2056097407 @default.
• W2136543503 cites W2060015752 @default.
• W2136543503 cites W2063220206 @default.
• W2136543503 cites W2065354024 @default.
• W2136543503 cites W2065458053 @default.
• W2136543503 cites W2082141778 @default.
• W2136543503 cites W2090194270 @default.
• W2136543503 cites W2090869432 @default.
• W2136543503 cites W2090895449 @default.
• W2136543503 cites W2108713158 @default.
• W2136543503 cites W2115136264 @default.
• W2136543503 cites W2129199209 @default.
• W2136543503 cites W2137802487 @default.
• W2136543503 cites W2143814603 @default.
• W2136543503 cites W2152278776 @default.
• W2136543503 cites W2165926160 @default.
• W2136543503 cites W2178562205 @default.
• W2136543503 doi "https://doi.org/10.1016/j.biomaterials.2013.10.023" @default.
• W2136543503 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24144907" @default.
• W2136543503 hasPublicationYear "2014" @default.
• W2136543503 type Work @default.
• W2136543503 sameAs 2136543503 @default.
• W2136543503 citedByCount "24" @default.
• W2136543503 countsByYear W21365435032013 @default.
• W2136543503 countsByYear W21365435032014 @default.
• W2136543503 countsByYear W21365435032015 @default.
• W2136543503 countsByYear W21365435032016 @default.
• W2136543503 countsByYear W21365435032017 @default.
• W2136543503 countsByYear W21365435032018 @default.
• W2136543503 countsByYear W21365435032019 @default.
• W2136543503 countsByYear W21365435032020 @default.
• W2136543503 countsByYear W21365435032021 @default.
• W2136543503 countsByYear W21365435032023 @default.
• W2136543503 crossrefType "journal-article" @default.
• W2136543503 hasAuthorship W2136543503A5014037848 @default.
• W2136543503 hasAuthorship W2136543503A5016205291 @default.
• W2136543503 hasAuthorship W2136543503A5035701091 @default.
• W2136543503 hasAuthorship W2136543503A5069539980 @default.
• W2136543503 hasAuthorship W2136543503A5085811442 @default.
• W2136543503 hasConcept C10138342 @default.
• W2136543503 hasConcept C12554922 @default.
• W2136543503 hasConcept C1491633281 @default.
• W2136543503 hasConcept C162324750 @default.
• W2136543503 hasConcept C171250308 @default.
• W2136543503 hasConcept C185592680 @default.
• W2136543503 hasConcept C189014844 @default.
• W2136543503 hasConcept C189165786 @default.
• W2136543503 hasConcept C192562407 @default.
• W2136543503 hasConcept C207200792 @default.
• W2136543503 hasConcept C2776964284 @default.
• W2136543503 hasConcept C3763915 @default.
• W2136543503 hasConcept C54400483 @default.
• W2136543503 hasConcept C55493867 @default.
• W2136543503 hasConcept C58943365 @default.
• W2136543503 hasConcept C60278653 @default.
• W2136543503 hasConcept C86492073 @default.
• W2136543503 hasConcept C86803240 @default.
• W2136543503 hasConcept C95444343 @default.
• W2136543503 hasConceptScore W2136543503C10138342 @default.
• W2136543503 hasConceptScore W2136543503C12554922 @default.
• W2136543503 hasConceptScore W2136543503C1491633281 @default.
• W2136543503 hasConceptScore W2136543503C162324750 @default.

• next