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• W2136563068 abstract "Silica fibres from the inflorescence bracts of the grass Phalaris canariensis L. cause dermatitis, and have been implicated in the aetiology of oesophageal cancer in northeastern Iran. Here we describe a method for labelling these fibres so that they can be located in mammalian tissue. Fluorescein was covalently linked to isolated, purified fibres with the silane coupling agent 3-aminopropyl triethoxysilane. The labelled hairs were then rubbed into the backs of mice. These were later killed and their skin fixed, stained and sliced at a thickness of 250 μm. A confocal laser scanning microscope gave brilliant images of the fibres at any depth up to 100 μm or more beneath the surface of the slice. Fibres penetrated deeply into the dermis. Several cubic millimetres of tissue could be surveyed in 1 h. The number of fibres present was approximately 2 mm−3 initially, falling to 0.1 mm−3 after 7 days." @default.
• W2136563068 created "2016-06-24" @default.
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• W2136563068 date "1994-04-01" @default.
• W2136563068 modified "2023-09-27" @default.
• W2136563068 title "DETECTING PLANT SILICA FIBRES IN ANIMAL TISSUE BY CONFOCAL FLUORESCENCE MICROSCOPY" @default.
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• W2136563068 doi "https://doi.org/10.1093/annhyg/38.2.149" @default.
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