Matches in SemOpenAlex for { <https://semopenalex.org/work/W2136820732> ?p ?o ?g. }
- W2136820732 endingPage "1411" @default.
- W2136820732 startingPage "1397" @default.
- W2136820732 abstract "The scaffold protein Vac14 acts in a complex with the lipid kinase PIKfyve and its counteracting phosphatase FIG4, regulating the interconversion of phosphatidylinositol-3-phosphate to phosphatidylinositol-3,5-bisphosphate. Dysfunctional Vac14 mutants, a deficiency of one of the Vac14 complex components, or inhibition of PIKfyve enzymatic activity results in the formation of large vacuoles in cells. How these vacuoles are generated and which processes are involved are only poorly understood. Here we show that ectopic overexpression of wild-type Vac14 as well as of the PIKfyve-binding deficient Vac14 L156R mutant causes vacuoles. Vac14-dependent vacuoles and PIKfyve inhibitor-dependent vacuoles resulted in elevated levels of late endosomal, lysosomal, and autophagy-associated proteins. However, only late endosomal marker proteins were bound to the membranes of these enlarged vacuoles. In order to decipher the linkage between the Vac14 complex and regulators of the endolysosomal pathway, a protein affinity approach combined with multidimensional protein identification technology was conducted, and novel molecular links were unraveled. We found and verified the interaction of Rab9 and the Rab7 GAP TBC1D15 with Vac14. The identified Rab-related interaction partners support the theory that the regulation of vesicular transport processes and phosphatidylinositol-modifying enzymes are tightly interconnected." @default.
- W2136820732 created "2016-06-24" @default.
- W2136820732 creator A5005517995 @default.
- W2136820732 creator A5029563848 @default.
- W2136820732 creator A5030484410 @default.
- W2136820732 creator A5033314506 @default.
- W2136820732 creator A5034945272 @default.
- W2136820732 creator A5044366967 @default.
- W2136820732 creator A5049644996 @default.
- W2136820732 creator A5049983824 @default.
- W2136820732 creator A5066255903 @default.
- W2136820732 creator A5080808515 @default.
- W2136820732 creator A5081126095 @default.
- W2136820732 creator A5086876078 @default.
- W2136820732 creator A5089460319 @default.
- W2136820732 date "2014-06-01" @default.
- W2136820732 modified "2023-10-15" @default.
- W2136820732 title "The Vac14-interaction Network Is Linked to Regulators of the Endolysosomal and Autophagic Pathway" @default.
- W2136820732 cites W1495616591 @default.
- W2136820732 cites W1590683934 @default.
- W2136820732 cites W1597678601 @default.
- W2136820732 cites W1630481045 @default.
- W2136820732 cites W1963524639 @default.
- W2136820732 cites W1964252660 @default.
- W2136820732 cites W1966282515 @default.
- W2136820732 cites W1966828940 @default.
- W2136820732 cites W1970661950 @default.
- W2136820732 cites W1970696723 @default.
- W2136820732 cites W1973024048 @default.
- W2136820732 cites W1976074435 @default.
- W2136820732 cites W1980828899 @default.
- W2136820732 cites W1994419078 @default.
- W2136820732 cites W1994668150 @default.
- W2136820732 cites W1995265959 @default.
- W2136820732 cites W1995943575 @default.
- W2136820732 cites W1996023830 @default.
- W2136820732 cites W2009518034 @default.
- W2136820732 cites W2012040176 @default.
- W2136820732 cites W2014375034 @default.
- W2136820732 cites W2017779665 @default.
- W2136820732 cites W2021710660 @default.
- W2136820732 cites W2023198417 @default.
- W2136820732 cites W2027974246 @default.
- W2136820732 cites W2031071878 @default.
- W2136820732 cites W2035398977 @default.
- W2136820732 cites W2036995295 @default.
- W2136820732 cites W2040337522 @default.
- W2136820732 cites W2041230129 @default.
- W2136820732 cites W2044089069 @default.
- W2136820732 cites W2045373134 @default.
- W2136820732 cites W2045493291 @default.
- W2136820732 cites W2045714699 @default.
- W2136820732 cites W2047275456 @default.
- W2136820732 cites W2047730219 @default.
- W2136820732 cites W2049848256 @default.
- W2136820732 cites W2054079210 @default.
- W2136820732 cites W2062562477 @default.
- W2136820732 cites W2066353226 @default.
- W2136820732 cites W2067638329 @default.
- W2136820732 cites W2072107269 @default.
- W2136820732 cites W2073057670 @default.
- W2136820732 cites W2076228191 @default.
- W2136820732 cites W2079046408 @default.
- W2136820732 cites W2082379606 @default.
- W2136820732 cites W2085820610 @default.
- W2136820732 cites W2090101918 @default.
- W2136820732 cites W2091238762 @default.
- W2136820732 cites W2091821324 @default.
- W2136820732 cites W2091884933 @default.
- W2136820732 cites W2097419416 @default.
- W2136820732 cites W2099936921 @default.
- W2136820732 cites W2110017950 @default.
- W2136820732 cites W2112640247 @default.
- W2136820732 cites W2112989471 @default.
- W2136820732 cites W2114366125 @default.
- W2136820732 cites W2115030877 @default.
- W2136820732 cites W2118278336 @default.
- W2136820732 cites W2120348985 @default.
- W2136820732 cites W2121023391 @default.
- W2136820732 cites W2126659352 @default.
- W2136820732 cites W2127063807 @default.
- W2136820732 cites W2127422378 @default.
- W2136820732 cites W2130706354 @default.
- W2136820732 cites W2131097779 @default.
- W2136820732 cites W2134748166 @default.
- W2136820732 cites W2135384508 @default.
- W2136820732 cites W2140324218 @default.
- W2136820732 cites W2140461742 @default.
- W2136820732 cites W2140758058 @default.
- W2136820732 cites W2145485258 @default.
- W2136820732 cites W2148184199 @default.
- W2136820732 cites W2148447088 @default.
- W2136820732 cites W2153607816 @default.
- W2136820732 cites W2156146280 @default.
- W2136820732 cites W2156840496 @default.
- W2136820732 cites W2159675211 @default.
- W2136820732 cites W2162126864 @default.
- W2136820732 cites W2163085584 @default.