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- W2136838638 abstract "Ultrafiltration combined with graphite furnace atomic absorption spectrometry (GFAAS) was used to study protein binding and speciation of copper in human serum. Ultrafiltration was carried out using a cell unit with ultrafiltration membranes having a nominal cut-off of 10,000 Dalton. The effects of various experimental factors including the kind and concentration of electrolyte, sample storage, pH. pressure and the preconditioning of the membranes on the speciation analysis of serum copper by ultrafiltration were examined. It was observed that 4.5 ± 2.3% of the total copper in serum was ultrafiltrable and this value did not seem to be influenced by the total serum elemental concentration, the pH (6.5–10) and the pressure (≤ 1.5 kg/cm2). The preconditioning of the ultrafiltration system with 0.1 mol/L calcium nitrate can overcome the adsorption loss of copper effectively, and the addition of tris-HCl solution (pH 7.4) to serum accelerates the ultrafiltration. The present method was proved to be suitable for speciation analysis for its simplicity, rapidity, small sample requirement and easy control. The results obtained with the method are accurate and reliable." @default.
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- W2136838638 date "2010-08-26" @default.
- W2136838638 modified "2023-09-23" @default.
- W2136838638 title "Speciation Analysis of Serum Copper by Ultrafiltration Combined with Graphite Furnace Atomic Absorption Spectrometry" @default.
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- W2136838638 doi "https://doi.org/10.1002/cjoc.20010190205" @default.
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