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- W2136875315 abstract "Recent years have seen the emergence of new high-throughput PCR and sequencing platforms with the potential to bring analysis of transcriptional biomarkers to a broader range of clinical applications and to provide increasing depth to our understanding of the transcriptome. We present an overview of how to process clinical samples for RNA biomarker analysis in terms of RNA extraction and mRNA enrichment, and guidelines for sample analysis by RT–qPCR and digital PCR using nanofluidic real-time PCR platforms. The options for quantitative gene expression profiling and whole transcriptome sequencing by next generation sequencing are reviewed alongside the bioinformatic considerations for these approaches. Considering the diverse technologies now available for transcriptome analysis, methods for standardising measurements between platforms will be paramount if their diagnostic impact is to be maximised. Therefore, the use of RNA standards and other reference materials is also discussed." @default.
- W2136875315 created "2016-06-24" @default.
- W2136875315 creator A5008886339 @default.
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- W2136875315 creator A5041020945 @default.
- W2136875315 creator A5052898193 @default.
- W2136875315 date "2013-01-01" @default.
- W2136875315 modified "2023-10-16" @default.
- W2136875315 title "Application of next generation qPCR and sequencing platforms to mRNA biomarker analysis" @default.
- W2136875315 cites W1530977725 @default.
- W2136875315 cites W1560628242 @default.
- W2136875315 cites W1963563809 @default.
- W2136875315 cites W1965175512 @default.
- W2136875315 cites W1967354629 @default.
- W2136875315 cites W1973214096 @default.
- W2136875315 cites W1973831834 @default.
- W2136875315 cites W1973839814 @default.
- W2136875315 cites W1979072451 @default.
- W2136875315 cites W1980989830 @default.
- W2136875315 cites W1981509058 @default.
- W2136875315 cites W1984984201 @default.
- W2136875315 cites W1986077582 @default.
- W2136875315 cites W1986080495 @default.
- W2136875315 cites W1986657646 @default.
- W2136875315 cites W1987470886 @default.
- W2136875315 cites W1988929438 @default.
- W2136875315 cites W1992608045 @default.
- W2136875315 cites W2002390964 @default.
- W2136875315 cites W2004869583 @default.
- W2136875315 cites W2007113062 @default.
- W2136875315 cites W2008594900 @default.
- W2136875315 cites W2008753573 @default.
- W2136875315 cites W2009458183 @default.
- W2136875315 cites W2010637767 @default.
- W2136875315 cites W2018248334 @default.
- W2136875315 cites W2019331497 @default.
- W2136875315 cites W2025955672 @default.
- W2136875315 cites W2026575873 @default.
- W2136875315 cites W2027099754 @default.
- W2136875315 cites W2027351830 @default.
- W2136875315 cites W2029069236 @default.
- W2136875315 cites W2029575024 @default.
- W2136875315 cites W2029961990 @default.
- W2136875315 cites W2030692069 @default.
- W2136875315 cites W2032395102 @default.
- W2136875315 cites W2034226898 @default.
- W2136875315 cites W2034355012 @default.
- W2136875315 cites W2038103024 @default.
- W2136875315 cites W2038230536 @default.
- W2136875315 cites W2039716761 @default.
- W2136875315 cites W2040361860 @default.
- W2136875315 cites W2043349549 @default.
- W2136875315 cites W2044010294 @default.
- W2136875315 cites W2052918843 @default.
- W2136875315 cites W2053184946 @default.
- W2136875315 cites W2055027171 @default.
- W2136875315 cites W2058805572 @default.
- W2136875315 cites W2060842569 @default.
- W2136875315 cites W2061360587 @default.
- W2136875315 cites W2061537056 @default.
- W2136875315 cites W2061560627 @default.
- W2136875315 cites W2061747833 @default.
- W2136875315 cites W2065476540 @default.
- W2136875315 cites W2074764083 @default.
- W2136875315 cites W2078757557 @default.
- W2136875315 cites W2080073306 @default.
- W2136875315 cites W2083322961 @default.
- W2136875315 cites W2083871583 @default.
- W2136875315 cites W2084813257 @default.
- W2136875315 cites W2088301231 @default.
- W2136875315 cites W2088925963 @default.
- W2136875315 cites W2094739251 @default.
- W2136875315 cites W2095244399 @default.
- W2136875315 cites W2096173343 @default.
- W2136875315 cites W2097117811 @default.
- W2136875315 cites W2097337885 @default.
- W2136875315 cites W2099468911 @default.
- W2136875315 cites W2100640908 @default.
- W2136875315 cites W2100703845 @default.
- W2136875315 cites W2101814635 @default.
- W2136875315 cites W2103753697 @default.
- W2136875315 cites W2104740044 @default.
- W2136875315 cites W2105514165 @default.
- W2136875315 cites W2105776281 @default.
- W2136875315 cites W2106335939 @default.
- W2136875315 cites W2109819853 @default.
- W2136875315 cites W2112119018 @default.
- W2136875315 cites W2112982595 @default.
- W2136875315 cites W2113312463 @default.
- W2136875315 cites W2113649367 @default.
- W2136875315 cites W2114504176 @default.
- W2136875315 cites W2118526609 @default.
- W2136875315 cites W2120994472 @default.
- W2136875315 cites W2121211805 @default.
- W2136875315 cites W2124308944 @default.
- W2136875315 cites W2125576153 @default.