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- W2136898493 abstract "To date, the common thread in the use of semen extenders/collection techniques, whether they are being used for fresh extended semen, chilled semen, or cryopreserved semen, is that the extenders have all traditionally been added post-collection. The objective of this study was to determine if modifying the method of collection/extension of semen to include a warmed media environment would improve semen parameters by lessening cold and pH shock. Ten canine semen samples were collected with a modified artificial vagina to allow for a true split collection into to collection containers at one time. The treatment half of the sample was collected into a measured amount of warmed extension media. The control half was collected into a dry container and no attempts to maintain temperature was used. Standard semen parameters, available sperm pool, and number of inseminations were evaluated at specific time intervals and evaluations continued until samples reached zero percent motility. Data analysis was performed with SPSS using the general linear model and appropriate t-tests. There was a difference between the treatment and control groups for Motility (P<.001), Motility by Time (P<.001), Time to Zero Motility (P<.001), Time to Last Full Insemination (P<.03), Forward Progression (P<.001), Acrosome Reaction (P<.001), Acrosome Reaction by Time (P<.02) and Viability (P<.001). There was no difference in morphology between the treatment and control groups (P>.05). Modification of the semen collection/extension procedure resulted in improved semen parameters for extended time-periods post-collection. The data suggest the described collection technique can yield significantly more motile sperm by placing the sample into a physiologically favorable environment (eliminating pH and cold shock and allowing osmoregulation to begin), thus providing more available sperm for breeding." @default.
- W2136898493 created "2016-06-24" @default.
- W2136898493 creator A5084638014 @default.
- W2136898493 date "2014-07-03" @default.
- W2136898493 modified "2023-10-14" @default.
- W2136898493 title "A Novel Collection Technique for the Improvement of Semen Quality" @default.
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- W2136898493 doi "https://doi.org/10.15406/jdvar.2014.01.00002" @default.
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