FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2137104501> ?p ?o ?g. }

• W2137104501 endingPage "e14" @default.
• W2137104501 startingPage "e14" @default.
• W2137104501 abstract "A substantial proportion of protein interactions relies on small domains binding to short peptides in the partner proteins. Many of these interactions are relatively low affinity and transient, and they impact on signal transduction. However, neither the number of potential interactions mediated by each domain nor the degree of promiscuity at a whole proteome level has been investigated. We have used a combination of phage display and SPOT synthesis to discover all the peptides in the yeast proteome that have the potential to bind to eight SH3 domains. We first identified the peptides that match a relaxed consensus, as deduced from peptides selected by phage display experiments. Next, we synthesized all the matching peptides at high density on a cellulose membrane, and we probed them directly with the SH3 domains. The domains that we have studied were grouped by this approach into five classes with partially overlapping specificity. Within the classes, however, the domains display a high promiscuity and bind to a large number of common targets with comparable affinity. We estimate that the yeast proteome contains as few as six peptides that bind to the Abp1 SH3 domain with a dissociation constant lower than 100 microM, while it contains as many as 50-80 peptides with corresponding affinity for the SH3 domain of Yfr024c. All the targets of the Abp1 SH3 domain, identified by this approach, bind to the native protein in vivo, as shown by coimmunoprecipitation experiments. Finally, we demonstrate that this strategy can be extended to the analysis of the entire human proteome. We have developed an approach, named WISE (whole interactome scanning experiment), that permits rapid and reliable identification of the partners of any peptide recognition module by peptide scanning of a proteome. Since the SPOT synthesis approach is semiquantitative and provides an approximation of the dissociation constants of the several thousands of interactions that are simultaneously analyzed in an array format, the likelihood of each interaction occurring in any given physiological settings can be evaluated. WISE can be easily extended to a variety of protein interaction domains, including those binding to modified peptides, thereby offering a powerful proteomic tool to help completing a full description of the cell interactome." @default.
• W2137104501 created "2016-06-24" @default.
• W2137104501 creator A5013874274 @default.
• W2137104501 creator A5018532546 @default.
• W2137104501 creator A5018540028 @default.
• W2137104501 creator A5025623787 @default.
• W2137104501 creator A5029338279 @default.
• W2137104501 creator A5032727709 @default.
• W2137104501 creator A5065510179 @default.
• W2137104501 date "2004-01-20" @default.
• W2137104501 modified "2023-10-13" @default.
• W2137104501 title "Protein Interaction Networks by Proteome Peptide Scanning" @default.
• W2137104501 cites W1575542444 @default.
• W2137104501 cites W1715675170 @default.
• W2137104501 cites W1892578325 @default.
• W2137104501 cites W1964048941 @default.
• W2137104501 cites W1972172786 @default.
• W2137104501 cites W1982334738 @default.
• W2137104501 cites W2006054867 @default.
• W2137104501 cites W2015961921 @default.
• W2137104501 cites W2019454936 @default.
• W2137104501 cites W2022257442 @default.
• W2137104501 cites W2031037573 @default.
• W2137104501 cites W2031907844 @default.
• W2137104501 cites W2037036397 @default.
• W2137104501 cites W2037425715 @default.
• W2137104501 cites W2055193239 @default.
• W2137104501 cites W2056189051 @default.
• W2137104501 cites W2059939467 @default.
• W2137104501 cites W2061507382 @default.
• W2137104501 cites W2065304353 @default.
• W2137104501 cites W2082426787 @default.
• W2137104501 cites W2084619201 @default.
• W2137104501 cites W2099982459 @default.
• W2137104501 cites W2109327803 @default.
• W2137104501 cites W2113654464 @default.
• W2137104501 cites W2137335580 @default.
• W2137104501 cites W2145080853 @default.
• W2137104501 cites W2149198894 @default.
• W2137104501 cites W2167869241 @default.
• W2137104501 cites W2318867253 @default.
• W2137104501 cites W2412454327 @default.
• W2137104501 doi "https://doi.org/10.1371/journal.pbio.0020014" @default.
• W2137104501 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/314469" @default.
• W2137104501 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14737190" @default.
• W2137104501 hasPublicationYear "2004" @default.
• W2137104501 type Work @default.
• W2137104501 sameAs 2137104501 @default.
• W2137104501 citedByCount "208" @default.
• W2137104501 countsByYear W21371045012012 @default.
• W2137104501 countsByYear W21371045012013 @default.
• W2137104501 countsByYear W21371045012014 @default.
• W2137104501 countsByYear W21371045012015 @default.
• W2137104501 countsByYear W21371045012016 @default.
• W2137104501 countsByYear W21371045012017 @default.
• W2137104501 countsByYear W21371045012018 @default.
• W2137104501 countsByYear W21371045012019 @default.
• W2137104501 countsByYear W21371045012020 @default.
• W2137104501 countsByYear W21371045012021 @default.
• W2137104501 countsByYear W21371045012022 @default.
• W2137104501 countsByYear W21371045012023 @default.
• W2137104501 crossrefType "journal-article" @default.
• W2137104501 hasAuthorship W2137104501A5013874274 @default.
• W2137104501 hasAuthorship W2137104501A5018532546 @default.
• W2137104501 hasAuthorship W2137104501A5018540028 @default.
• W2137104501 hasAuthorship W2137104501A5025623787 @default.
• W2137104501 hasAuthorship W2137104501A5029338279 @default.
• W2137104501 hasAuthorship W2137104501A5032727709 @default.
• W2137104501 hasAuthorship W2137104501A5065510179 @default.
• W2137104501 hasBestOaLocation W21371045011 @default.
• W2137104501 hasConcept C104317684 @default.
• W2137104501 hasConcept C104397665 @default.
• W2137104501 hasConcept C108636557 @default.
• W2137104501 hasConcept C11804247 @default.
• W2137104501 hasConcept C144292202 @default.
• W2137104501 hasConcept C150194340 @default.
• W2137104501 hasConcept C167625842 @default.
• W2137104501 hasConcept C186268636 @default.
• W2137104501 hasConcept C196347352 @default.
• W2137104501 hasConcept C2776471321 @default.
• W2137104501 hasConcept C2779281246 @default.
• W2137104501 hasConcept C2910703447 @default.
• W2137104501 hasConcept C46111723 @default.
• W2137104501 hasConcept C51639874 @default.
• W2137104501 hasConcept C55493867 @default.
• W2137104501 hasConcept C62478195 @default.
• W2137104501 hasConcept C70721500 @default.
• W2137104501 hasConcept C86803240 @default.
• W2137104501 hasConcept C94795543 @default.
• W2137104501 hasConcept C95371953 @default.
• W2137104501 hasConcept C95444343 @default.
• W2137104501 hasConceptScore W2137104501C104317684 @default.
• W2137104501 hasConceptScore W2137104501C104397665 @default.
• W2137104501 hasConceptScore W2137104501C108636557 @default.
• W2137104501 hasConceptScore W2137104501C11804247 @default.
• W2137104501 hasConceptScore W2137104501C144292202 @default.
• W2137104501 hasConceptScore W2137104501C150194340 @default.
• W2137104501 hasConceptScore W2137104501C167625842 @default.

• next